The objective of this paper is to investigate variability in chemical composition, total fatty acid and cholesterol content in m. longissimus dorsi (MLD) of Mangalitsa, swallow-belly (LM) and white (BM), and Swedish Landrace pigs (SL). Compared to SL, the total fat content has been 14.2% higher in BM, while it has been 10.2% higher in LM. SL fatteners contained significantly less cholesterol in MLD compared to LM and BM (−13.6 and −14.8%, P≤0.05). A higher percentage of SFA (+8.5 and +10.1%, P≤0.05) and PUFA (+8.0 and +9.4%, P≤0.05) has been established in MLD, originating from SL fatteners, compared to both Mangalitsa strains. The total MUFA content was higher in LM and BM than in SL (P≤0.05). A phenotypic correlation between protein content and ashes with water content in MLD has been positive (0.81 and 0.88), while the correlation between water content and total fats has been found to be negative (−0.99). A negative phenotypic correlation between MUFA and SFA, as well as PUFA and MUFA (−0.97 and −0.98) has been established, statistically significant at the level of P≤0.001. A positive phenotypic correlation between PUFA and SFA (0.90), statistically significant at the level of P≤0.001, has been found.