Zygosaccharomyces lentus is a significant, recently discovered new osmophilic spoilage yeast, capable of growth at low temperatures. It is remarkably resistant to commonly used preservatives above the legally permitted levels. Therefore, antimicrobial effect of chitosan, cinnamic acid, carvacrol and cymene as new preservatives was tested against Z. lentus in phosphate buffered saline at pH 7.3. Z. lentus was resistant to the antimicrobial action of chitosan (0.5 and 1.0%) and cinnamic acid (0.5–2.0 mM) but was sensitive to the biocidal action of 1.5 and 2.0 mM cymene and 1.5 and 2.0 mM carvacrol in pH-neutral buffer. Further work would need to be done to check the sensitivity of this organism to these compounds at other pH values and temperatures.