Glycosyltransferase activity of Pectinex ultra SP-L (from Aspergillus aculeatus) commercially available enzyme preparation was studied in mono- and bisubstrate systems using different donors (maltose, lactose, and sucrose) and acceptors (fructose, galactose, glucose, maltose, mannose, xylose, lactose, and sucrose). Oligosaccharides consisting of three monomers were detected in the cases of maltose, sucrose, and lactose as monosubstrates, thus this preparation should contain glucosyl-, fructosyl-, and galactosyl-transferase activity. Generally, yields of oligosaccharides synthesized were higher in bisubstrate systems maltose:sucrose, maltose:lactose, and sucrose:lactose than on monosubstrates. Use of maltose:sucrose bisubstrate resulted new oligosaccharide(s) (fructosyl-maltose or glucosyl-sucrose). The optimal ratio of substrates in the case of maltose:sucrose was determined to be 1:9. Increase of the dry content of the reaction mixture induced the transfer reaction. The highest oligosaccharide content was obtained at 60% (w/v) substrate concentration. In the case of the optimal ratio and dry content, 4.02% (w/v) oligosaccharide (DP3) concentration was achieved. These oligosaccharides may alter prebiotic and biochemical properties in food applications.
Antošovă, M. & Polakovič, M. (2001): Fructosyltransferases: The enzymes catalyzing production of fructooligosaccharides. Chem. Pap., 55, 350–358.
Polakovič M., 'Fructosyltransferases: The enzymes catalyzing production of fructooligosaccharides' (2001) 55Chem. Pap.: 350-358.
Polakovič M.Fructosyltransferases: The enzymes catalyzing production of fructooligosaccharidesChem. Pap.200155350358)| false
Beine, R., Moraru, R., Nimtz, M., Na’amnieh, S., Pawlowski, A., Buchholz, K. & Seibel, J. (2008): Synthesis of novel fructooligosaccharides by substrate and enzyme engineering. J. Biotechnol., 138, 33–41.
Seibel J., 'Synthesis of novel fructooligosaccharides by substrate and enzyme engineering' (2008) 138J. Biotechnol.: 33-41.
Seibel J.Synthesis of novel fructooligosaccharides by substrate and enzyme engineeringJ. Biotechnol.20081383341)| false
Ghazi, I., Fernandez-Arrojo, L., Garcia-Arellano, H., Ferrer, M., Ballestoros, A. & Plou, F.J. (2007): Purification and kinetic characterization of fructosyltransferase from Aspergillus aculeatus. J. Biotechnol., 128, 204–211.
Plou F.J., 'Purification and kinetic characterization of fructosyltransferase from Aspergillus aculeatus' (2007) 128J. Biotechnol.: 204-211.
Plou F.J.Purification and kinetic characterization of fructosyltransferase from Aspergillus aculeatusJ. Biotechnol.2007128204211)| false
Homann, A., Biedendick, R., Götze, S., Jahn, D. & Seibel, J. (2007): Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium. Biochem. J., 407, 189–198.
Seibel J., 'Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium' (2007) 407Biochem. J.: 189-198.
Seibel J.Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megateriumBiochem. J.2007407189198)| false
Maitin, V. & Rastall, R.A. (2007): Enzymatic synthesis of oligosaccharides: Progress and recent trends. -in: Shetty, K., Paliyath, G., Pometto, A.L. & Levin, R.E. (Eds): Functional foods and biotechnology. CRC Press, Taylor & Francis Group, Boca Raton U.S., pp. 473–500.
Rastall R.A., '', in Functional foods and biotechnology, (2007) -.
Rastall R.A.Functional foods and biotechnology2007)| false
Monsan, P. & Paul, F. (1995): Enzymatic synthesis of oligosaccharides. FEMS Microbiol. Reviews, 16, 187–192.
Paul F., 'Enzymatic synthesis of oligosaccharides' (1995) 16FEMS Microbiol. Reviews: 187-192.
Paul F.Enzymatic synthesis of oligosaccharidesFEMS Microbiol. Reviews199516187192)| false
Nguyen, D.Q., Mattes, F., Hoschke, Á., Rezessy-Szabó, J. & Bhat, M.K. (1999): Production, purification and identification of fructooligosaccharides produced by β-fructofuranosidase from Aspergillus niger IMI 303386. Biotechnol. Letters, 21, 183–186.
Bhat M.K., 'Production, purification and identification of fructooligosaccharides produced by β-fructofuranosidase from Aspergillus niger IMI 303386' (1999) 21Biotechnol. Letters: 183-186.
Bhat M.K.Production, purification and identification of fructooligosaccharides produced by β-fructofuranosidase from Aspergillus niger IMI 303386Biotechnol. Letters199921183186)| false
Rodrigez-Fernandez, M., Cardelle-Cobas, A., Villamiel, M. & Banga, J.R. (2011): Detailed kinetic model describing new oligosaccharides synthesis using different β-galactosidases. J. Biotechnol., 153, 116–124.
Banga J.R., 'Detailed kinetic model describing new oligosaccharides synthesis using different β-galactosidases' (2011) 153J. Biotechnol.: 116-124.
Banga J.R.Detailed kinetic model describing new oligosaccharides synthesis using different β-galactosidasesJ. Biotechnol.2011153116124)| false
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2021 Volume 50
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