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  • 1 Budapest University of Technology and Economics Department of Applied Biochemistry and Food Science H-1111 Budapest Szent Gellért tér 4 Hungary
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The need to introduce promising bioethanol production technologies calls for advanced laboratory techniques to study experiment designs and to obtain their results in a quick and reliable way. Real time monitoring based on general principles of ethanol fermentation, such as effluent CO2 volume, avoids time consuming steps, long lasting analyses and delivers information about the process directly. A device based on the above features and capable for real time monitoring on parallel channels was developed by the authors and is described in this paper. Both for calibration and for fermentation, test runs were carried out on different days and channels. Statistical evaluation was based on the obtained data. According to the t-test (P=0.05) and Grubbs analysis, the calibration method is reliable regardless of the date of calibration. When evaluating the fermentation results by ANCOVA acceptable standard derivations were obtained as impact of channel (58.8 ml), date (82.1 ml) and incorporating all impacts (116.2 ml). The final ethanol concentrations calculated based on the gas volume were compared to ones determined by HPLC and an average difference of 10% was found. Thus, the device proved to be advantageous in monitoring fermentation.

  • Guwy, A.J., Hawkes, D.L. & Hawkes, F.R. (1995): On-line low flow high-precision gas metering systems. Water Research, 29, 977–979.

    Hawkes F.R. , 'On-line low flow high-precision gas metering systems ' (1995 ) 29 Water Research : 977 -979.

    • Search Google Scholar
  • Johnson, E.A. & Echavarri-Erasun, C. (2011): Yeast biotechnology. -in: Kurtzman, C.P., Fell, J.W. & Boekhout, T. (Eds) The yeasts. A taxonomic study (5th ed.), Elsevier BV. New York, pp. 21–44.

    Echavarri-Erasun C. , '', in The yeasts. A taxonomic study , (2011 ) -.

  • Lei, Y., Chen, W. & Ashok, M. (2006): Microbial biosensors. Anal. Chim. Acta, 568, 200–210.

    Ashok M. , 'Microbial biosensors ' (2006 ) 568 Anal. Chim. Acta : 200 -210.

  • Liu, J., Olsson, G. & Mattiasson, B. (2004): A volumetric meter for monitoring of low gas flow rate from laboratoryscale biogas reactors. Sensors Actuators B, 97, 369–372.

    Mattiasson B. , 'A volumetric meter for monitoring of low gas flow rate from laboratoryscale biogas reactors ' (2004 ) 97 Sensors Actuators B : 369 -372.

    • Search Google Scholar
  • Macías, M., Pérez, M., Caro, I. & Cantero, D. (1995): Automatic gas meter for laboratory ferments. Biotechnol. Techniques, 9, 655–658.

    Cantero D. , 'Automatic gas meter for laboratory ferments ' (1995 ) 9 Biotechnol. Techniques : 655 -658.

    • Search Google Scholar
  • Russell, I. (2003): Understanding yeast fundamentals. -in: Jacques, K.A., Lyons, T.P. & Kelsall, D.R. (Eds) The alcohol textbook. A reference for the beverage, fuel and industrial alcohol industries (4th ed.), Nottingham University Press Nottingham, UK, pp. 85–120.

  • Sanchez, O.J. & Cardona, C.A. (2008): Trends in biotechnological production of fuel ethanol from different feedstock. Bioresource Technol., 99, 5270–5295.

    Cardona C.A. , 'Trends in biotechnological production of fuel ethanol from different feedstock ' (2008 ) 99 Bioresource Technol : 5270 -5295.

    • Search Google Scholar
  • Smith, S.A. & Stöckle, C.O. (2008): A biogas meter with adjustable resolution and minimal back-pressure. Bioresource Technol., 99, 8537–8539.

    Stöckle C.O. , 'A biogas meter with adjustable resolution and minimal back-pressure ' (2008 ) 99 Bioresource Technol : 8537 -8539.

    • Search Google Scholar
  • Veiga, M.C., Soto, M., Méndez, R. & Lema, J.M. (1990): A new device for measurement and control of gas production by bench scale anaerobic digesters. Water Res., 24, 1551–1554.

    Lema J.M. , 'A new device for measurement and control of gas production by bench scale anaerobic digesters ' (1990 ) 24 Water Res : 1551 -1554.

    • Search Google Scholar
  • Young, J.C., Kuss, L.M. & Nelson, A.M. (1991): Use of anaerobic respirometers for measuring gas production in toxicity and treatability tests. Proceedings of the 84th Annual Meeting of the Air and Waste Management Association. Vancouver BC, Canada.

    Nelson A.M. , '', in Proceedings of the 84th Annual Meeting of the Air and Waste Management Association , (1991 ) -.

  • Yulin, L. & Mosier, N.S. (2008): Current technologies for fuel ethanol production from lignocellulosic plant biomass. -in: Vermerris, W. (Ed.) Genetic improvement of bioenergy crops. Springer Science + Business Media, LLC, New York, NY, USA, pp. 161–182.

    Mosier N.S. , '', in Genetic improvement of bioenergy crops , (2008 ) -.

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