View More View Less
  • 1 Spain Regional Campus of International Excellence “Campus Mare Nostrum”, 30.100 Murcia
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

The aim of this research was to assess the total antioxidant activity (TAA) of lipophilic (Lextr) and hydrophilic (Hextr) tomato extracts using in vitro chemical tests and cell-based assays, focusing on possible synergistic actions between tomato antioxidants. Both Hextr and Lextr were HPLC analysed for their carotenoids, phenolic compounds, and ascorbic acid contents. For the evaluation of TAA, extracts were assayed alone or in combination using in vitro chemical tests (TEAC, FRAP) and cell-based (CAA) assays using human hepatoma (HepG2) and human histiocytic lymphoma (U937) cells. The only carotenoid detected in Lextr was lycopene, while a mixture of phenolic compounds (chlorogenic acid, caffeic acid, and rutin) was identified in Hextr. Ascorbic acid was not found either in Hextr or in Lextr. Upon extract combination (1:1, v/v), the FRAP assay revealed additive action between Lextr and Hextr, whilst a slight synergistic action was observed in TAA as measured by the TEAC assay. Synergistic action was better revealed when TAA was analysed using either U937 or HepG2 cells. This could be explained by the presence of a multiphase media (cell membrane and extra- and intracellular media) that might facilitate the distribution and interaction of antioxidants with different polarities and different mechanisms of action.

  • Alía, M., Ramos, S., Mateos, R., Bravo, L. & Goya, L. (2005): Response of the antioxidant defense system to tertbutyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J. Biochem. Mol. Toxicol., 19, 119128.

    • Search Google Scholar
    • Export Citation
  • Benzie, I.F.F. & Strain, J.J. (1996): The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal. Biochem., 239, 7076.

    • Search Google Scholar
    • Export Citation
  • Blasa, M., Angelino, D., Gennari, L. & Ninfali, P. (2011): The cellular antioxidant activity in red blood cells (CAARBC): A new approach to bioavailability and synergy of phytochemicals and botanical extracts. Food Chem., 125, 685691.

    • Search Google Scholar
    • Export Citation
  • Böhm V. (2001): Use of column temperature to optimise carotenoid isomer separation by C30 high performance liquid chromatography. J. Sep. Sci., 24, 955959.

    • Search Google Scholar
    • Export Citation
  • Esteve, M.J., Farré, R., Frijola, A., López, J.C., Romera, J.M., RAMíREZ, M. & Gil, A. (1995): Comparison of voltammetric and high performance liquid chromatographic methods for ascorbic acid determination in infant formulas. Food Chem., 52, 99102.

    • Search Google Scholar
    • Export Citation
  • García-Alonso, F.J., Guidarelli, A. & Periago, M.J. (2007): Phenolic-rich juice prevents DNA single-strand breakage and cytotoxicity caused by tert-butylhydroperoxide in U937 cells: the role of iron chelation. J. Nutr. Biochem., 18, 457466.

    • Search Google Scholar
    • Export Citation
  • Jacob, K., Periago, M.J., Böhm, V. & Ros-Berruezo, G. (2008): Influence of lycopene and vitamin C from tomato juice on biomarkers of oxidative stress and inflammation. Br. J. Nutr., 99, 137146.

    • Search Google Scholar
    • Export Citation
  • Kotíková, Z., Lachman, J., Hejtmánková, A. & Hejtmánková, K. (2011): Determination of antioxidant activity and antioxidant content in tomato varieties and evaluation of mutual interactions between antioxidants. LWT –Food Sci. Technol., 44, 17031710.

    • Search Google Scholar
    • Export Citation
  • Laguerre, M., Lecomte, J. & Villeneuve, P. (2007): Evaluation of the ability of antioxidants to counteract lipid oxidation: Existing methods, new trends and challenges. Prog. Lipid Res., 46, 244282.

    • Search Google Scholar
    • Export Citation
  • Liu, R.H. (2003): Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am. J. Clin. Nutr., 78, 517520.

    • Search Google Scholar
    • Export Citation
  • Manach, C., Williamson, G., Morand, C., Scalbert, A. & Remesy, C. (2004): Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am. J. Clin. Nutr., 81, 230242.

    • Search Google Scholar
    • Export Citation
  • Miller, N.J., Sampson. J., Candeias, L.P. , Bramley, P.M. & Rice-Evans, C.A. (1996): Antioxidant activities of carotenes and xanthophylls. FEBS Lett., 384, 240242.

    • Search Google Scholar
    • Export Citation
  • Mosmann, T. (1983): Rapid colorimetric assay for cellular growth and survival: Application to proliferation and citotoxicity assays. J. Inmunol. Methods, 65, 5563.

    • Search Google Scholar
    • Export Citation
  • Murakami, A., Takahashi, D., Koshimizu, K. & Ohigashi, H. (2003): Synergistic suppression of superoxide and nitric oxide generation from inflammatory cells by combined food factors. Mutat. Res., 523–524, 151161.

    • Search Google Scholar
    • Export Citation
  • Navarro-González, I., García-Valverde, V., García-Alonso, J. & Periago, M.J. (2011): Chemical profile, functional and antioxidant properties of tomato peel fiber. Food Res. Int., 44, 15281535.

    • Search Google Scholar
    • Export Citation
  • Periago, M.J., Garcia-Alonso, F.J., Jacob, K., Olivares, A.B., Bernal, M.J., Iniesta, M.D., Martinez, C. & Ros, G. (2009): Bioactive compounds, folates and antioxidant properties of tomatoes (Lycopersicum esculentum) during vine ripening. Int. J. Food Sci. Nutr., 60, 694670.

    • Search Google Scholar
    • Export Citation
  • Sies, H. & Stahl, W. (1995): Vitamins E and C, beta-carotene, and other carotenoids as antioxidants. Am. J. Clin. Nutr., 62, 13151321.

    • Search Google Scholar
    • Export Citation
  • Vetrani, C., Costabile, G., Di Marino, L. & Rivellese, A.A. (2013): Nutrition and oxidative stress: a systematic review of human studies. Int. J. Food Sci. Nutr., 64, 312326.

    • Search Google Scholar
    • Export Citation
  • Wang, M., Meckling, K.A., Marcone, M.F., Kakuda, Y. & Tsao, R. (2011): Synergistic, additive, and antagonistic effects of food mixtures on total antioxidant capacities. J. Agr. Food Chem., 5, 960968.

    • Search Google Scholar
    • Export Citation
  • Wolfe, K.L. & Liu, R.U. (2007): Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods and dietary supplements. J. Agr. Food Chem., 55, 88968907.

    • Search Google Scholar
    • Export Citation
  • Zanfini, A., Corbini, G., LA Rosa, C. & Dreassi, E. (2010): Antioxidant activity of tomato lipophilic extracts and interactions between carotenoids and α-tocopherol in synthetic mixtures. LWT – Food Sci. Technol., 43, 6772.

    • Search Google Scholar
    • Export Citation
All Time Past Year Past 30 Days
Abstract Views 84 83 3
Full Text Views 30 5 0
PDF Downloads 20 1 0