View More View Less
  • a Akdeniz University, Dumlupinar Boulevard, 07058, Campus, Antalya, Turkey
  • b Alanya Hamdullah Emin Paşa University, Cikcilli Beldesi Saraybeleni Area, Saraybeleni Street. No:7, 07400, Antalya, Turkey
  • c Kocaeli University, Kocaeli Cayirova Road, Campus, 041380, Kocaeli, Turkey
  • d Middle East Technical University, University Area, Dumlupinar Boulevard No:1, 06800, Campus, Ankara, Turkey
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

β-mannanases can degrade galactomannans to mannose and it has been used in various application areas. The aim of this study was to produce the β-mannanase from carob pod extract including different nitrogen sources. The best operation values for fermentation were determined to be 8% initial sugar concentration with 0.5% yeast extract, 100 r.p.m., and 7% inoculation rate, which yielded the maximum β-mannanase activity as 423.60 U ml–1. Effects of nitrogen sources on β-mannanase activity were also studied and it reached 695.6 U ml−1 by using 0.5% of ammonium nitrate as the nitrogen source at the determined optimum conditions. Results also showed that meat bone meal and soybean meal could be used as cost effective nitrogen sources based on achieved β-mannanase activity.

  • BOX, G.E.P. & BEHNKEN, D.W. (1960): Some new three level designs for study of quantitative variables. Technometrics, 2, 455475.

  • CHEN, X., CAO, Y., DING, Y., LU, W. & LI, D. (2007): Cloning, functional expression and characterization of Aspergillus sulphures ß-mannanase in Pichia pastoris. J. Biotechnol., 128, 452461.

    • Search Google Scholar
    • Export Citation
  • DHAWAN, S., SINGH, R., KAUR, R. & KAUR, J. (2015): A ß-mannanase from Paenibacillus sp.: Optimization of production and its possible prebiotic potential. Biotechnol. Appl. Biochem., Jul 29. DOI: 10.1002/bab.1419.

    • Search Google Scholar
    • Export Citation
  • DURUKSU, G., OZTURK, B., BIELY, P., BAKIR, U. & OGEL, Z.B. (2009): Cloning, expression and characterization of endo-beta-1,4-mannanase from Aspergillus fumigatus in Aspergillus sojae and Pichia pastoris. Biotechnol. Progr., 25, 271276.

    • Search Google Scholar
    • Export Citation
  • EL-SHAROUNY, E.E., EL-TOUKHY, N.M., EL-SERSY, N.A. & EL-GAYAR, A.A.E.A. (2015): Optimization and purification of mannanase produced by an alkaliphilic-thermotolerant Bacillus cereus N1 isolated from Bani Salama Lake in Wadi El-Natron. Biotechnol. Biotec. Eq., 29(2), 315323.

    • Search Google Scholar
    • Export Citation
  • HUANG, J.L., BAO, L.X., ZOU, H.Y., CHE, S.G. & WANG, G.X. (2012): High-level production of a cold-active ß-mannanase from Bacillus subtilis Bs5 and its molecular cloning and expression. Mol. Genet. Microbiol. Virol., 27(4), 147153.

    • Search Google Scholar
    • Export Citation
  • KARKACIER, M., ARTIK, N. & CERTEL, M. (1995): The conditions for carob (Ceratonia siliqua L.) extraction and clarification of the extract. Fruit Process, 12, 394397.

    • Search Google Scholar
    • Export Citation
  • KATROLIA, P., YAN, Q., ZHANG, P., ZHOU, P., YANG, S. & JIANG, Z. (2013): Gene cloning and enzymatic characterization of an alkali-tolerant endo-1,4-ß-mannanase from Rhizomucor miehei. J. Agr. Food Chem., 61, 394401.

    • Search Google Scholar
    • Export Citation
  • KURAKAKE, M. & KOMAKI, T. (2001): Production of ß-mannanase and ß-mannosidase from Aspergillus awamori K4 and their properties. Curr. Microbiol., 42(6), 377380.

    • Search Google Scholar
    • Export Citation
  • LIN, S.S., DOU, W.F., XU, H.Y., LI, H.Z., XU, Z.H. & MA, Y.H. (2007): Optimization of medium composition for the production of alkaline ß-mannanase by alkaliphilic Bacillus sp. N16-5 using response surface methodology. Appl. Microbiol. Biot., 75, 10151022.

    • Search Google Scholar
    • Export Citation
  • LU, H., LUO, H., SHI, P., HUANG, H., MENG, K., YANG, P. & YAO, B. (2014): A novel thermophilic endo-ß-1,4- mannanase from Aspergillus nidulans XZ3: functional roles of carbohydrate-binding module and Thr/Ser rich linker region. Appl. Microbiol. Biot., 98, 21552163.

    • Search Google Scholar
    • Export Citation
  • LUO, H., WANG, K., HUANG, H., SHI, P., YANG, P. & YAO, B. (2012): Gene cloning, expression, and biochemical characterization of an alkali-tolerant ß-mannanase from Humicola insolens Y1. J. Ind. Microbiol. Biot., 39, 547555.

    • Search Google Scholar
    • Export Citation
  • MILLER, G.L. (1959): Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426.

  • OZTURK, B. (2008): Optimization of mannanase production from recombinant Aspergillus sojae and analysis of galactomannan hydrolysis. Master’s thesis. Middle East Technical University, Turkey.

    • Search Google Scholar
    • Export Citation
  • OZTURK, B., CEKMECELIOGLU, D. & OGEL, Z.B. (2010): Optimal conditions for enhanced ß-mannanase production by recombinant Aspergillus sojae. J. Mol. Catal. B-Enzym., 64, 135139.

    • Search Google Scholar
    • Export Citation
  • SONI, H., GANAIE, M.A., PRANAW, K. & KANGO, N. (2015): Design-of-experiment strategy for the production of mannanase biocatalysts using palm kernel cake and its application to degrade locust bean and guar gum. Biocatal. Agr. Biotechnol. (BAB), 97, 101110.

    • Search Google Scholar
    • Export Citation
  • TURHAN, I., BIALKA, K.L., DEMIRCI, A. & KARHAN, M. (2010a): Ethanol production from carob pod extract by using Saccharomyces cerevisiae. Bioresource Technol., 101, 52905296.

    • Search Google Scholar
    • Export Citation
  • TURHAN, I., BIALKA, L.K., DEMIRCI, A. & KARHAN, M. (2010b): Enhanced lactic acid production from carob extract by Lactobacillus casei using invertase pretreatment. Food Biotechnol., 24, 364374.

    • Search Google Scholar
    • Export Citation
  • VAN ZYL, W.H., ROSE, S.H., TROLLOPE, K. & GORGENS, J.F. (2010): Fungal ß-mannanases: Mannan hydrolysis, heterologous production and biotechnological applications. Process Biochem., 45, 12031213.

    • Search Google Scholar
    • Export Citation
  • WANG, J., SHAO, Z., HONG, Y., LI, C., FU, X. & LIU, Z. (2010): A novel ß-mannanase from Pantoea agglomerans A021: Gene cloning, expression, purification and characterization. World J. Microb. Biot., 26, 17771784.

    • Search Google Scholar
    • Export Citation
  • WU, M., TANG, C., LI, J., ZHANG, H. & GUO, J. (2011): Bimutation breeding of Aspergillus niger strain for enhancing ß-mannanase production by solid-state fermentation. Carbohyd. Res., 346, 21492155.

    • Search Google Scholar
    • Export Citation
  • YIN, L.J., TAI, H.M. & JIANG, S.T. (2012a): Characterization of mannanase from a novel mannanase-producing bacterium. J. Agr. Food Chem., 60, 64256431.

    • Search Google Scholar
    • Export Citation
  • YIN, J.S., LIANG, Q.L., LI, D.M. & SUN, Z.T. (2012b): Optimization of production conditions for ß-mannanase using apple pomace as raw material in solid-state fermentation. Ann. Microbiol., 63, 101108.

    • Search Google Scholar
    • Export Citation
  • ZHAO, W., ZHENG, J. & ZHOU, H. (2011): A thermotolerant and cold-active mannan endo-1,4-ß-mannosidase from Aspergillus niger CBS 513.88: Constitutive overexpression and high density fermentation in Pichia pastoris. Bioresource Technol., 102, 75387547.

    • Search Google Scholar
    • Export Citation
  • ZHENG, J., ZHAO, W., GUO, N., LIN, F., TIAN, J., WU, L. & ZHOU, H. (2012): Development of an industrial medium and a novel fed-batch strategy for high-level expression of recombinant ß-mannanase by Pichia pastoris. Bioresource Technol., 118, 257264.

    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 6 0 0
Sep 2020 13 0 0
Oct 2020 9 0 0
Nov 2020 10 2 1
Dec 2020 6 0 0
Jan 2021 8 0 0
Feb 2021 0 0 0