The present study was to evaluate the survival rate of free and encapsulated Bifidobacterium bifidum BB28 under simulated gastrointestinal conditions and its stability during storage. Results showed that non-microencapsulated Bifidobacterium bifidum BB28 was more susceptible to simulated gastrointestinal conditions than microencapsulated bacteria. Microencapsulated Bifidobacterium BB28 exhibited a lower population reduction than free cells during exposure to simulated gastrointestinal conditions, the viable count of monolayer microcapsules, double layer microcapsules, and triple layer microcapsules decreased by nine magnitudes, four magnitudes, and one magnitude after 2 h, respectively. The enteric test showed that the microorganism cells were released from the monolayer, double layer, and triple layer microcapsules completely in 40 min. Moreover, the optimum storage times of free Bifidobacterium BB28, monolayer microcapsules, double layer microcapsules, and triple layer microcapsules were 21 days, 21 days, 28 days, and more than 35 days in orange juice, pure milk, and nutrition Express (a commercially available milk based drink), and the viable counts were maintained at 1×106 CFU g−1 or more, which means that the double layer and triple layer of microcapsules of B. bifidum BB28 have great potential in food application.
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