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  • a Sant Longowal Institute of Engineering and Technology, Longowal-148106, Punjab, India
  • b Punjabi University, Patiala 147 002 , India
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β-Galactosidase is an enzyme of commercial importance owing to its multiple benefits. Among all microbial sources, fungal species are of great interest for the production of this enzyme. Thus, the aim of this present work was to optimize the media as well as process parameters to achieve maximum β-Galactosidase production by solid state fermentation using the fungal isolate Rhizomucor pusillus. Various agro-industrial residues were tested for carbon as well as for nitrogen sources. The different process parameters were also studied to observe their effects on β-galactosidase production. Among all screened agro-industrial residues, wheat bran and corn steep liquor had the potential to be used as carbon and nitrogen sources, respectively; whereas MgSO4 was found to be a suitable salt supplement. The optimal process parameters included particle size of 1000 microns, 50% moisture content, pH 5.5, 50 ºC temperature, and 7 days of fermentation.

  • Akcan, N. (2011): High level production of extracellular β-galactosidase from Bacillus licheniformis ATCC 12759 in submerged fermentation. Afr. J. Microbiol. Res. 5(26), 46154621.

    • Search Google Scholar
    • Export Citation
  • Anisha, G.S., Sukumaran, R.K. & Prema, P. (2008): Evaluation of α-galactosidase biosynthesis by Streptomyces griseoalbus in solid state fermentation. Lett. Appl. Microbiol., 46, 338345.

    • Search Google Scholar
    • Export Citation
  • Assamoi, A.A., Bedikou, M.E., Soro-Yao, A.A., Niamke, L.S., Destain, J. & Thonart, P. (2015): β-Galactosidase production by solid state fermentation of wheat bran/whole wheat without any supplement. WJPPS, 4, 196207.

    • Search Google Scholar
    • Export Citation
  • Awan, M.S., Khan, S.A., Rehman, Z.U., Saleem, A., Rana, S.M. & Rajoka M.I. (2010a): Influence of nitrogen sources on production of β-galactosidase by Aspergillus niger. Afr. J. Biotechnol., 9(20), 29182922.

    • Search Google Scholar
    • Export Citation
  • Awan, M.S., Tabbasam, N., Ayub, N., Babar, M.E., Rahman, M., Rana, S.M. & Rajoka, M.I. (2010b): Gamma radiation induced mutagenesis in Aspergillus niger to enhance its microbial fermentation activity for industrial enzyme production. Mol. Biol. Rep., 38, 13671374.

    • Search Google Scholar
    • Export Citation
  • Craig, D.B., Hall, T. & Goltz, T.M. (2000): Escherichia coli and α-D galactosidase is heterogenous with respect to a requirement of magnesium. Biometals, 13, 223229.

    • Search Google Scholar
    • Export Citation
  • El-Gindy, A., Ibrahim, Z. & Aziz, H. (2009): Improvement of extracellular β-galactosidase production by thermophilic fungi Chaetomium thermophile and Thermomyces lanuginosus. AJBAS, 3, 19251932.

    • Search Google Scholar
    • Export Citation
  • Hatzinikolaou, D.G., Katsifas, E., Mamma, D., Karagouni, A.D., Christakopoulos, P. & Kekos, D. (2005): Modeling of the simultaneous hydrolysis-ultrafiltration of whey permeate by a thermostable β-galactosidase from Aspergillus niger. Biochem. Eng. J., 24, 161172.

    • Search Google Scholar
    • Export Citation
  • Jurado, E., Camacho, F., Luzan, G. & Vicaria, J.M. (2004): Kinetic models of activity for β-galactosidases: influence of pH, ionic concentration and temperature. Enzym. Microb. Tech., 34, 3340.

    • Search Google Scholar
    • Export Citation
  • Karpen, J.W. & Ruiz, M.L. (2002): Ion channels: Does each subunit do something on its own? Trends Biochem. Sci., 27, 402409.

  • Kumari, S., Panesar, P.S. & Panesar, R. (2011): Production of β-galactosidase using novel yeast isolate from whey. Int. J. Dairy Sci., 6, 150157.

    • Search Google Scholar
    • Export Citation
  • Mekala, N.K., Singhania, R.R., Sukumaran, R.K. & Pandey, A. (2008): Cellulase production under solid state fermentation by Trichoderma reesei RUT C30: Statistical optimization of process parameters. Appl. Biochem. Biotech., 151, 122131.

    • Search Google Scholar
    • Export Citation
  • Nizamuddin, S., Sridevi, A. & Narasimha, G. (2008): Production of β-galactosidase by Aspergillus oryzae in solidstate fermentation. Afr. J. Biotechnol., 7, 10961100.

    • Search Google Scholar
    • Export Citation
  • Raol, G.G., Raol, B.V., Prajapati, V.S. & Bhavsar, N.H. (2015): Utilization of agro-industrial waste for β-galactosidase production under solid state fermentation using halotolerant Aspergillus tubingensis GR1 isolate. 3 Biotech., 5, 411421.

    • Search Google Scholar
    • Export Citation
  • Reczey, K., Stalbrand, H., Hahn-Hegerdal, B. & Tijernal, F. (1992): Mycelia associated β-galactosidase activity in microbial pellets of Aspergillus and Penicillium strains. Appl. Microbiol. Biot., 38, 393397.

    • Search Google Scholar
    • Export Citation
  • Rodrigueź, C.S. (2008): Exploitation of biological wastes for the production of value added products under solid state fermentation conditions. Biotechnol. J., 3, 859870.

    • Search Google Scholar
    • Export Citation
  • Sen, P., Nath, A., Bhattacharjee, C., Chowdhury, R. & Bhattacharya, P. (2014): Process engineering studies of free and micro-encapsulated β-galactosidase in batch and packed bed bioreactors for production of galactooligosaccharides. Biochem. Eng. J., 90, 5972.

    • Search Google Scholar
    • Export Citation

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