View More View Less
  • a Southwest University, Chongqing 400715, P.R. China
  • b Chongqing Three Gorges University, Chongqing 404100, P.R. China
  • c Southwest University, Chongqing 400715, P.R. China
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

The present study was performed to investigate the effect of β-aminobutyric acid (BABA) treatment on defence activation in grape berries and to analyse its cellular mechanism. The results implied that BABA treatment at an effective concentration of 20 mM significantly inhibited gray mould rot caused by Botrytis cinerea in grape berries by inducing resistance. Accordingly, 20 mM BABA triggered a priming defence in grape suspension cells, since only the BABA-treated cells exhibited an accelerated ability for augmenting defence responses upon the pathogen inoculation. The primed cellular reactions were related to an early H2O2 burst, prompt accumulation of stilbene phytoalexins and activation of PR genes. Thus, we assume that 20 mM BABA can induce resistance to B. cinerea infection in intact grape berries perhaps via intercellular priming defence. Moreover, the BABA-induced priming defence is verified, because no negative effects on cell growth, anthocyanin synthesis, and quality impairment in either grape cells or intact berries were observed under low pathogenic pressure.

  • Abeles, F.B., Bosshart, R.P., Forrence, L.E. & Habig, W.H. (1971): Preparation and purification of glucanase and chitinase from bean leaves. Plant Physiol., 47, 129134.

    • Search Google Scholar
    • Export Citation
  • Aziz, A., Trotel-Aziz, P., Dhuicq, L., Jeandet, P., Couderchet, M. & Vernet, G. (2006): Chitosan oligomers and copper sulfate induce grapevine defence reactions and resistance to gray mold and downy mildew. Phytopathology, 96, 11881194.

    • Search Google Scholar
    • Export Citation
  • Belchí-Navarro, S., Almagro, L., Sabater-Jara, A.B., Fernández-Pérez, F., Bru, R. & Pedreño, M.A. (2013): Early signaling events in grapevine cells elicited with cyclodextrins and methyl jasmonate. Plant Physiol. Bioch., 62, 107110.

    • Search Google Scholar
    • Export Citation
  • Cheng, G.W. & Breen, P.J. (1991): Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. J. Am. Soc. Hortic. Sci., 116, 865869.

    • Search Google Scholar
    • Export Citation
  • Cohen, Y., Vaknin, M. & Mauch-Mani, B. (2016): BABA-induced resistance: milestones along a 55-year journey. Phytoparasitica, 44, 513538.

    • Search Google Scholar
    • Export Citation
  • Conrath, U., Beckers, G.J., Langenbach, C.J. & Jaskiewicz, M.R. (2015): Priming for enhanced defense. Annu. Rev. Phytopathol., 53, 97119.

    • Search Google Scholar
    • Export Citation
  • El-Metwally, M.A., Tarabih, M.E. & El-Eryan, E.E. (2014): Effect of application of β-aminobutyric acid on maintaining quality of crimson seedless grape and controlling postharvest diseases under cold storage condition. Plant Pathology J., 3, 139151.

    • Search Google Scholar
    • Export Citation
  • Gozzo, F. & Faoro, F. (2013): Systemic acquired resistance (50 years after discovery): Moving from the lab to the field. J. Agr. Food Chem., 61, 1247312491.

    • Search Google Scholar
    • Export Citation
  • Granado, J., Felix, G. & Boller, T. (1995): Perception of fungal sterols in plants (subnanomolar concentrations of ergosterol elicit extracellular alkalinization in tomato cells). Plant Physiol., 107, 485490.

    • Search Google Scholar
    • Export Citation
  • Huot, B., Yao, J., Montgomery, B.L. & He, S.Y. (2014): Growth-defense tradeoffs in plants: a balancing act to optimize fitness. Mol. Plant, 7, 12671287.

    • Search Google Scholar
    • Export Citation
  • Le Henanff, G., Farine, S., Kieffer-Mazet, F., Miclot, A.S., Heitz, T., Mestre, P., Bertsch, C. & Chong, J. (2011): Vitis vinifera VvNPR1.1 is the functional ortholog of AtNPR1 and its overexpression in grapevine triggers constitutive activation of PR genes and enhanced resistance to powdery mildew. Planta, 234, 405417.

    • Search Google Scholar
    • Export Citation
  • Liao, Y.X., Fei, L.H., Xia, M.X., Wu, D.Z., Chen, S. & Wang, K.T. (2018): A study on different modes of disease resistance response induced by β-aminobutyric acid in grape berries. Food Science, 39, 221228 (in Chinese with English abstract).

    • Search Google Scholar
    • Export Citation
  • Livak, K.J. & Schmittgen, T.D. (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25, 402408.

    • Search Google Scholar
    • Export Citation
  • Martinez-Esteso, M.J., Sellés-Marchart, S., Vera-Urbina, J.C., Pedreño, M.A. & Bru-Martinez, R. (2009): Changes of defense proteins in the extracellular proteome of grapevine (Vitis vinifera cv. Gamay) cell cultures in response to elicitors. J. Proteomics, 73, 331341.

    • Search Google Scholar
    • Export Citation
  • O'Brien, J.A., Daudi, A., Butt, V.S. & Bolwell, G.P. (2012): Reactive oxygen species and their role in plant defence and cell wall metabolism. Planta, 236, 765779.

    • Search Google Scholar
    • Export Citation
  • Patterson, B.D., MacRae, E.A. & Ferguson, I.B. (1984): Estimation of hydrogen peroxide in plant extracts using titanium. Anal. Biochem., 139, 487492.

    • Search Google Scholar
    • Export Citation
  • Romanazzi, G., Sanzani, S.M., Bi, Y., Tian, S., Martínez, P.G. & Alkan, N. (2016): Induced resistance to control postharvest decay of fruit and vegetables. Postharvest Biol. Tec., 122, 8294.

    • Search Google Scholar
    • Export Citation
  • Van Hulten, M., Pelser, M., Van Loon, L.C., Pieterse, C.M.J. & Ton, J. (2006): Costs and benefits of priming for defense in Arabidopsis. P. Natl. Acad. Sci. USA, 104, 56025607.

    • Search Google Scholar
    • Export Citation
  • Wang, K., Liao, Y., Cao, S., Di, H. & Zheng, Y. (2015): Effects of benzothiadiazole on disease resistance and soluble sugar accumulation in grape berries and its possible cellular mechanisms involved. Postharvest Biol. Tec., 102, 5160.

    • Search Google Scholar
    • Export Citation
  • Wang, K., Liao, Y., Xiong, Q., Kan, J., Cao, S. & Zheng, Y. (2016): Induction of direct or priming resistance against Botrytis cinerea in strawberries by β-aminobutyric acid and their effects on sucrose metabolism. J. Agr. Food Chem., 64, 58555865.

    • Search Google Scholar
    • Export Citation
  • Zhang, C., Wang, J., Zhang, J., Hou, C. & Wang, G. (2011): Effects of β-aminobutyric acid on control of postharvest blue mould of apple fruit and its possible mechanisms of action. Postharvest Biol. Tec., 61, 145151.

    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 34 4
Jul 2020 17 0 0
Aug 2020 46 0 0
Sep 2020 15 0 0
Oct 2020 25 0 0
Nov 2020 33 7 1
Dec 2020 0 0 0