Plant-based proteins are being rapidly explored in the food industry to produce healthy and nutritious foods. Herein, the effects of soy protein isolate (SPI) ratio to oat germ powder (OGP) (0:0, 3.7: 6.3, 5:5, 0:10, 10:0, and 6.3:3.7%) were studied on the physicochemical, colour, rheological, microbial, antioxidant, and sensory properties of wheyless cheese. The results showed that by increasing SPI and OGP levels, ash, white index value, elastic modulus, and complex viscosity decreased, while pH, fat in dry matter, Chroma, and total colour (ΔE) increased. Also, syneresis was only observed in the control sample and was not observed in other samples. Control sample together with sample containing 10% of OGP showed the highest microbial count (i.e., mesophilic bacteria and yeasts-moulds), while samples containing 10% of SPI showed the lowest microbial count. Furthermore, the growth of both coliform and psychrophilic bacteria in all samples was negligible. Based on the results of sensory evaluation, with increasing SPI and OGP, the sensory evaluation score of samples decreased but they were within the acceptable range. According to the results obtained in this study, the combinations of 5% SPI and 5% OGP or 3.7% SPI and 6.3% OGP are recommended to be used in wheyless cheese production.
Anli, E.A., Gursel, A., Gursoy, A., and Mert, B. (2023). Assessment of the quality attributes of oat β-glucan fortified reduced-fat goat milk yogurt supported by microfluidization. Foods, 12(18): 3457. https://doi.org/10.3390/foods12183457.
AOAC (2005). Official methods of analysis, 18th ed. Association of Official Analytical Chemists, Washington DC, Methods No. 933.05 and 920.123.
Atia, M., Wenshui, X., and Guonong, Z. (2004). Effect of soy protein supplementation on the quality of ripening cheddar-type cheese. International Journal of Dairy Technology, 57(4): 209–214. https://doi.org/10.1111/j.1471-0307.2004.00107.x.
Bachmann, H.P. (2001). Cheese analogues: a review. International Dairy Journal, 11(4–7): 505–515. https://doi.org/10.1016/S0958-6946(01)00073-5.
Bağcı, A., Geçgel, Ü., Özcan, M.M., Dumlupınar, Z., and Uslu, N. (2019). Oil contents and fatty acid composition of oat (Avena sativa L) seed and oils. Journal of Agroalimentary Processes and Technologies, 25: 182–186.
Batool, M., Nadeem, M., Imran, M., Gulzar, N., Shahid, M.Q., Shahbaz, M., Ajmal, M., and Khan, I.T. (2018). Impact of vitamin E and selenium on antioxidant capacity and lipid oxidation of cheddar cheese in accelerated ripening. Lipids in Health and Disease, 17(1): 1–14. https://doi.org/10.1186/s12944-018-0735-3.
Bostanabad, M.K., Bolandi, M., Nafchi, A.M., and Baghaei, H. (2022). Wheyless cheese: an alternative method to reduce the environmental hazards of Lighvan cheese production. Journal of Food and Bioprocess Engineering, 5: 93–98. https://doi.org./10.22059/jfabe.2022.345145.1122.
Farahnaky, A., Mousavi, S.H., and Nasiri, M. (2013). Role of salt in Iranian ultrafiltered Feta cheese: some textural and physicochemical changes during ripening. International Journal of Dairy Technology, 66(3): 359–365. https://doi.org/10.1111/1471-0307.12051.
Gholamhosseinpour, A.A., Tehrani, M.M., and Razavi, S.M.A. (2018). Optimization of textural characteristics of analogue UF-Feta cheese made from dairy and non-dairy ingredients. Iranian Food Science and Technology Research Journal, 13(6): 80–91. https://ifstrj.um.ac.ir/article_36505.html?lang=en.
Golchin, N., Jafarian, S., Ghaboos, S.H.H., and Nasiraie, L.R. (2023). Optimization of cheese analogue formulation with rice milk, chia seed and hazelnut oil applying response surface methodology. Journal of Research and Innovation in Food Science and Technology, 11(4): 423–436. https://doi.org/10.22101/jrifst.2022.343004.1361.
Hamad, M.N.E.F., El-Bushuty, D.H., and El-Zakzouk, H.S. (2020). Manufacture of functional Kareish cheese fortified with oat Taliban lima bean and sweet lupine. Egyptian Journal of Food Science, 48(2): 315–326. https://ejfs.journals.ekb.eg/article_117012.html.
Hamdy, S.M., Hassan, M.G., Ahmed, R.B., and Abdelmontaleb, H.S. (2021). Impact of oat flour on some chemical physicochemical and microstructure of processed cheese. Journal of Food Processing and Preservation, 45(9): e15761. https://doi.org/10.1111/jfpp15761.
Hussein, G.A.M. and Shalaby, S.M. (2018). Properties of imitation cheese products prepared with non-dairy ingredients. The Saudi Journal of Life Sciences, 3: 578–587. https://doi.org/10.21276/haya.2018.3.9.2.
Khalid, N.T. and Mosa, M.A. (2018). Effect of wheat germ on chemical sensory and technological properties of soft cheese. International Journal of Dairy Science, 13: 40–45. https://doi.org/10.3923/ijds.2018.40.45.
Khang, D.T., Vasiljevic, T., and Xuan, T.D. (2016). Bioactive compounds, antioxidant and enzyme activities in germination of oats (Avena sativa L). International Food Research Journal, 23: 1980–1987. http://www.ifrj.upm.edu.my/23%20(05)%202016/(20).pdf.
Khiabanian, N.O., Motamedzadegan, A., Raisi, S.N., and Alimi, M. (2020). Chemical textural rheological and sensorial properties of whey-less feta cheese as influenced by replacement of milk protein concentrate with pea protein isolate. Journal of Texture Studies, 51(3): 488–500. https://doi.org/10.1111/jtxs.12508.
Khiabanian, N.O., Motamedzadegan, A., Raisi, S.N., and Alimi, M. (2022). Structure–rheology characterization of whey-less feta cheese containing milk protein concentrate/soy protein isolate. Korea-Australia Rheology Journal, 34: 35–49. https://doi.org/10.1007/s13367-022-00020-3.
Kim, S.Y., Park, P.S.W., and Rhee, K.C. (1992). Textural properties of cheese analogs containing proteolytic enzyme-modified soy protein isolates. Journal of the American Oil Chemists' Society, 69(8): 755–759. https://doi.org/10.1007/BF02635911.
Kordialik-Bogacka, E., Bogdan, P., and Diowksz, A. (2014). Malted and unmalted oats in brewing. Journal of the Institute of Brewing, 120(4): 390–398. https://doi.org/10.1002/jib.178.
Kubo, M.T.K., Maus, D., Xavier, A.A.O., Mercadante, A.Z., and Viotto, W.H. (2013). Transference of lutein during cheese making colour stability and sensory acceptance of Prato cheese. Food Science and Technology, 33(Suppl.1): 82–88. https://doi.org/10.1590/S0101-20612013000500013.
Lee, C.H., Yang, L., Xu, J.Z., Ying, S., Yeung, V., Huang, Y., and Chen, Z.Y. (2005). Relative antioxidant activity of soybean isoflavones and their glycosides. Food Chemistry, 90(4): 735–741. https://doi.org/10.1016/j.foodchem.2004.04.034.
Ma, W., Qi, B., Sami, R., Jiang, L., Li, Y., and Wang, H. (2018). Conformational and functional properties of soybean proteins produced by extrusion-hydrolysis approach. International Journal of Analytical Chemistry, 2018: 9182508. https://doi.org/10.101155/2018/9182508.
Mazinani, S., Motamedzadegan, A., Raeisi, S.N., and Alimi, M. (2021). Characterization of bacteriologically acidified feta cheese using soy protein isolate in different substitution percentages: rheological microbiological and sensory properties. Journal of Food Measurement and Characterization, 15(6): 5515–5527. https://doi.org/10.1007/s11694-021-00973-z.
Murphy, P.A., Song, T., Buseman, G., Barua, K., Beecher, G.R., Trainer, D., and Holden, J. (1999). Isoflavones in retail and institutional soy foods. Journal of Agricultural and Food Chemistry, 47(7): 2697–2704. https://pubs.acs.org/doi/10.1021/jf981144o.
Palupi, N.S., Prangdimurt, E., Faridah, D.N., and Asyhari, M.H. (2020). Reducing allergenicity of soy protein isolate from several varieties of soybean through glycation with lactose. Current Research in Nutrition and Food Science, 8(1): 268–280. https://doi.org/10.12944/CRNFSJ.8.1.25.
Paudel, D., Dhungana, B., Caffe, M., and Krishnan, P. (2021). A review of health-beneficial properties of oats. Foods, 10(11): 2591. https://doi.org/10.3390/foods10112591.
Peng, Y., Kyriakopoulou, K., Ndiaye, M., Bianeis, M., Keppler, J.K., and van der Goot, A.J. (2021). Characteristics of soy protein prepared using an aqueous ethanol washing process. Foods, 10(9): 2222. https://doi.org/10.3390/foods10092222.
Prag, A.A. and Henriksen, C.B. (2020). Transition from animal-based to plant-based food production to reduce greenhouse gas emissions from agriculture - the case of Denmark. Sustainability, 12(19): 8228. https://doi.org/10.3390/su12198228.
Rahman, M.B.M., Islam, M.B., Biswas, A.H.M.K.M., and Alam, B.M.C. (2015). In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Research Notes, 8(1): 621. https://doi.org/10.1186/s13104-015-1618-6.
Rinaldoni, A.N., Palatnik, D.R., Zaritzky, N., and Campderrós, M.E. (2014). Soft cheese-like product development enriched with soy protein concentrates. LWT – Food Science and Technology, 55(1): 139–147. https://doi.org/10.1016/j.lwt.2013.09.003.
Rojas-Nery, E., Güémes-Vera, N., Meza-Marquez, O., and Totosaus, A. (2015). Carrageenan type effect on soybean oil/soy protein isolate emulsion employed as fat replacer in panela-type cheese. Grasas y Aceites, 66: e097. https://doi.org/10.3989/gya0240151.
Sen, M.A., Palabiyik, I., and Kurultay, S. (2019). The effect of saleps obtained from various Orchidaceae species on some physical and sensory properties of ice cream. Food Science and Technology Campinas, 39(1): 83–87. https://doi.org/10.1590/fst.26017.
Singh, R., De, S., and Belkheir, A. (2013). Avena sativa (oat) a potential nutraceutical and therapeutic agent: an overview. Critical Reviews in Food Science and Nutrition, 53(2): 126–144. https://doi.org/10.1080/10408398.2010.526725.
Soleimani-Rambod, A., Zomorodi, S., Raeisi, S.N., Khosrowshahi, Asl.A., and Shahidi, S.A. (2018). The effect of xanthan gum and flaxseed mucilage as edible coatings in cheddar cheese during ripening. Coatings, 8(2): 80. https://doi.org/10.3390/coatings8020080.
Song, D.H., Lee, K.J., and An, J.H. (2024). Antioxidant activity and volatile components of sprouted oat beer. LWT, 193: 115757. https://doi.org/10.1016/j.lwt.2024.115757.
Tian, B., Xie, B., Shi, J., Wu, J., Cai, Y., Xu, T., Xue, S., and Deng, Q. (2010). Physicochemical changes of oat seeds during germination. Food Chemistry, 119(3): 1195–1200. https://doi.org/10.1016/j.foodchem.2009.08.035.
Trmčić, A., Chauhan, K., Kent, D.J., Ralyea, R.D., Martin, N.H., Boor, K.J., and Wiedmann, M. (2016). Coliform detection in cheese is associated with specific cheese characteristics but no association was found with pathogen detection. Journal of Dairy Science, 99(8): 6105–6120. https://doi.org/10.3168/jds.2016-11112.
Tungmunnithum, D., Thongboonyou, A., Pholboon, A., and Yangsabai, A. (2018). Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: an overview. Medicines, 5(3): 93. https://doi.org/10.3390/medicines5030093.
Zomorodi, S., Azarpazhooh, E., and Behmadi, H. (2020). Influence of some hydrocolloids on textural properties of UF cheese. Journal of Food Biosciences and Technology, 10(2): 1–10.