Both onion and shallot are the most common ingredients to use for many recipes. Consuming onion and shallot provide health benefits including antidiabetic effects. However, the benefits of onion and shallot mixture at different ratios have not been studied in terms of total phenolic content, total antioxidant capacity, superoxide dismutase (SOD)-like activity, and antidiabetic effects. Our study revealed that shallot significantly increased total phenolic content in the mixture from 492 ± 32 μg mL−1 (100% v/v onion) to 803 ± 24 μg mL−1 (100% v/v shallot) depending on the ratios of shallot in the mixture. Shallot also significantly enhanced total antioxidant capacity, SOD-like activity, α-amylase inhibition, and α-glucosidase inhibition in the mixture. Shallot juice exhibited the highest percentage inhibition of α-amylase activity (40.51 ± 1.57%) and α-glucosidase activity (89.61 ± 2.85%) in comparison to those of onion juice and the mixture. In addition, significant positive correlation between phenolic compound and different variables was observed (P < 0.05). Phenolic compounds present in onion and shallot are probably responsible for many health benefits including antidiabetic effects.
Aguirre, L., Arias, N., Macarulla, M.T., Gracia, A., and Portillo, M.P. (2011). Beneficial effects of quercetin on obesity and diabetes. Open Nutraceuticals Journal, 4: 189–198.
Aryal, D., Joshi, S., Thapa, N.K., Chaudhary, P., Basaula, S., Joshi, U., Bhandari, D., Rogers, H.M., Bhattarai, S., Sharma, K.R., Regmi, B.P., and Parajuli, N. (2024). Dietary phenolic compounds as promising therapeutic agents for diabetes and its complications: a comprehensive review. Food Science & Nutrition, 00: 1–21, Online version of record before inclusion in an issue, https://doi.org/10.1002/fsn3.3983.
Balasundram, N., Sundram, K., and Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1): 191–203.
Beidokht, M.N. and Jäger, A.K. (2017). Review of antidiabetic fruits, vegetables, beverages, oils and spices commonly consumed in the diet. Journal of Ethnopharmacology, 201: 26–41.
Beretta, H.V., Bannoud, F., Insani, M., Berli, F., Hirschegger, P., Galmarini, C.R., and Cavagnaro, P.F. (2017). Relationships between bioactive compound content and the antiplatelet and antioxidant activities of six Allium vegetable species. Food Technology and Biotechnology, 55(2): 266–275.
Colina-Coca, C., Gonzalez-Pena, D., de Ancos, B., and Sanchez-Moreno, C. (2017). Dietary onion ameliorates antioxidant defense, inflammatory response, and cardiovascular risk biomarkers in hypercholesterolemic Wistar rats. Journal of Functional Foods, 36: 300–309.
Dona, A.C., Pages, G., Gilbert, R.G., and Kuchel, P.W. (2010). Digestion of starch: In vivo and in vitro kinetic models used to characterise oligosaccharide or glucose release. Carbohydrate Polymers, 80(3): 599–617.
Eldin, I.M.T., Ahmed, E.M., and Elwahab, A.H.M. (2010). Preliminary study of the clinical hypoglycemic effects of Allium cepa (red onion) in type 1 and type 2 diabetic patients. Environmental Health Insights, 4: 71–77.
Kiani, Z., Hassanpour-Fard, M., Asghari, Z., and Hosseini, H. (2018). Experimental evaluation of a polyherbal formulation (Tetraherbs): antidiabetic efficacy in rats. Comparative Clinical Pathology, 27(6): 1437–1445.
Lu, X., Wang, J., Al-Qadiri, H.M., Ross, C.F., Powers, J.R., Tang, J., and Rasco, B.A. (2011). Determination of total phenolic content and antioxidant capacity of onion (Allium cepa) and shallot (Allium oschaninii) using infrared spectroscopy. Food Chemistry, 129(2): 637–644.
Luangpirom, A., Kourchampa, W., Junaimuang, T., Somsapt, P., and Sritragool, O. (2013). Effect of shallot (Allium ascalonicum L.) bulb juice on hypoglycemia and sperm quality in streptozotocin induced diabetic mice. Animal Biology & Animal Husbandry Bioflux, 5(1): 49–54.
Mannucci, E., Monami, M., Lamanna, C., and Adalsteinsson, J.E. (2012). Post-prandial glucose and diabetic complications: systematic review of observational studies. Acta Diabetologica, 49(4): 307–314.
Mansuroğlu, B., Derman, S., Yaba, A., and Kızılbey, K. (2015). Protective effect of chemically modified SOD on lipid peroxidation and antioxidant status in diabetic rats. International Journal of Biological Macromolecules, 72: 79–87.
Masood, S., Rehman, A.U., Bashir, S., El Shazly, M., Imran, M., Khalil, P., Ifthikar, F., Jaffar, H.M., and Khursheed, T. (2021a). Investigation of the anti-hyperglycemic and antioxidant effects of wheat bread supplemented with onion peel extract and onion powder in diabetic rats. Journal of Diabetes and Metabolic Disorders, 20(1): 485–495.
Masood, S., Rehman, A.U., Ihsan, M.A., Shahzad, K., Sabir, M., Alam, S., Ahmed, W., Shah, Z.H., Alghabari, F., Mehmood, A., and Chung, G. (2021b). Antioxidant potential and α-glucosidase inhibitory activity of onion (Allium cepa L.) peel and bulb extracts. Brazilian Journal of Biology, 83: 00264.
Mathew, P.T. and Augusti, K.T. (1975). Hypoglycemic effects of onion, Allium cepa Linn. on diabetes mellitus - a preliminary report. Indian Journal of Physiology and Pharmacology, 19(4): 213–217.
Oyedemi, S.O., Oyedemi, B.O., Ijeh, I.I., Ohanyerem, P.E., Coopoosamy, R.M., and Aiyegoro, O.A. (2017). Alpha-amylase inhibition and antioxidative capacity of some antidiabetic plants used by the traditional healers in Southeastern Nigeria. The Scientific World Journal, 2017: 3592491.
Perumal, N., Nallappan, M., Shohaimi, S., Kassi, N.K., Tee, T.T., and Cheah, Y.H. (2022). Synergistic antidiabetic activity of Taraxacum officinale (L.) Weber ex F.H. Wigg and Momordica charantia L. polyherbal combination. Biomedicine and Pharmacotherapy, 145: 112401.
Rasouli, H., Hosseini-Ghazvini, S.M.B., Adibi, H., and Khodarahmi, R. (2017). Differential alpha-amylase/alpha-glucosidase inhibitory activities of plant-derived phenolic compounds: a virtual screening perspective for the treatment of obesity and diabetes. Food and Function, 8(5): 1942–1954.
Sarian, M.N., Ahmed, Q.U., Mat So'ad, S.Z., Alhassan, A.M., Murugesu, S., Perumal, V., Syed Mohamad, S.N.A., Khatib, A., and Latip, J. (2017). Antioxidant and antidiabetic effects of flavonoids: a structure-activity relationship based study. BioMed Research International, 2017: 8386065.
Shahrajabian, M.H., Sun, W., and Cheng, Q. (2020). Chinese onion, and shallot, originated in Asia, medicinal plants for healthy daily recipes. Notulae Scientia Biologicae, 12(2): 197–207.
Singleton, V.L., Orthofer, R.Y., and Lamuela-Raventós, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299: 152–178.
Sun, L., Song, Y., Chen, Y., Ma, Y., Fu, M., and Liu, X. (2021). The galloyl moiety enhances the inhibitory activity of catechins and the aflavins against α-glucosidase by increasing the polyphenol-enzyme binding interactions. Food & Function, 12(1): 215–229.
Sun, L., Warren, F.J., and Gidley, M.J. (2019). Natural products for glycemic control: polyphenols as inhibitors of alpha-amylase. Trends in Food Science & Technology, 91: 262–273.
Valcheva-Kuzmanova, S., Gadjeva, V., Ivanova, D., and Belcheva, A. (2007). Antioxidant activity of Aronia melanocarpa fruit juice in vitro. Acta Alimentaria, 36(4): 425–428.
Wongsa, P., Chaiwarit, J., and Zamaludien, A. (2012). In vitro screening of phenolic compounds, potential inhibition against α-amylase and α-glucosidase of culinary herbs in Thailand. Food Chemistry, 131(3): 964–971.
Wu, H. and Xu, B. (2014). Inhibitory effects of onion against α-glucosidase activity and its correlation with phenolic antioxidants. International Journal of Food Properties, 17(3): 599–609.
Zhang, H., Wang, G., Beta, T., and Dong, J. (2015). Inhibitory properties of aqueous ethanol extracts of propolis on alpha-glucosidase. Evidence-Based Complementary and Alternative Medicine, 2015: 587383.