Acta Alimentaria
Volume/Issue:
Accepted Manuscript / Online First
Establishment of a high-sensitivity indirect competitive ELISA for florfenicol in chicken meat and egg
Authors:
M. Ge Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China

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Y. Xing Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

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Y. Sun Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

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W. Cai Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China

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Y. Wang College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China

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https://orcid.org/0000-0002-7193-3276
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X. Hu Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

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https://orcid.org/0000-0002-5728-1021
Online Publication Date:
08 Apr 2025
Publication Date:
08 Apr 2025
Article Category:
Research Article
DOI:
https://doi.org/10.1556/066.2024.00220
Keywords:
florfenicol; high sensitivity; ic-ELISA; chicken meat; egg
Restricted access

Abstract

The aim was to develop a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method for detecting florfenicol (FF) in chicken meat and eggs. The concentrations of the coated antigen and monoclonal antibodies of FF and the reaction conditions were optimised. The performance of the established method was thoroughly evaluated. The results indicated that the detection limit (LOD) was 0.011 μg kg−1 with an IC50 of 0.224 μg kg−1 and a detection range of 0.011–4.538 μg kg−1. The coefficient of variation between and within batches was less than 10%. The spiked recoveries of chicken meat samples ranged from 90.10 to 95.55%, while the spiked recoveries of egg samples ranged from 91.28 to 97.56%. In conclusion, the developed ic-ELISA method is highly sensitive and suitable for monitoring and detecting trace FF residues in chicken meat samples.

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  • Tao, X., Yu, X., Zhang, D., Shi, W., Jiang, H., Wang, X., and Shen, J. (2014). Development of a rapid chemiluminescent ci-ELISA for simultaneous determination of florfenicol and its metabolite florfenicol amine in animal meat products. Journal of the Science of Food and Agriculture, 94(2): 301–307, https://doi.org/10.1002/jsfa.6258.

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  • Wang, W., Zhang, Y., and Yang, H. (2022). Development of a nucleocapsid protein-based blocking ELISA for the detection of porcine deltacoronavirus antibodies. Viruses, 14(8): 1815, https://doi.org/10.3390/v14081815.

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  • Yang, X., Wang, Y., Song, C., Hu, X., Wang, F., and Zeng, X. (2020). Hapten synthesis and the development of an ultrasensitive indirect competitive ELISA for the determination of diethylstilbestrol in food samples. Scientific Reports, 10(1): 3270, https://doi.org/10.1038/s41598-020-59112-1.

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  • Yin, M., Hu, X., Sun, Y., Xing, Y., Chai, S., Xing, G., and Zhang, G. (2020). The broad-spectrum and ultra-sensitive detection of zeranol and its analogues by an enzyme-linked immunosorbent assay in cattle origin samples. Royal Society of Chemistry Advances, 10(35): 20809–20816, https://doi.org/10.1039/d0ra02936j.

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Cover Acta Alimentaria
Acta Alimentaria
Print ISSN:
0139-3006
Online ISSN:
1588-2535

Senior editors

Editor(s)-in-Chief: András Salgó, Budapest University of Technology and Economics, Budapest, Hungary

Co-ordinating Editor(s) Marianna Tóth-Markus, Budapest, Hungary

Co-editor(s): A. Halász, Budapest, Hungary

       Editorial Board

  • László Abrankó, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
  • Tamás Antal, University of Nyíregyháza, Nyíregyháza, Hungary
  • Diána Bánáti, University of Szeged, Szeged, Hungary
  • József Baranyi, Institute of Food Research, Norwich, UK
  • Ildikó Bata-Vidács, Eszterházy Károly Catholic University, Eger, Hungary
  • Ferenc Békés, FBFD PTY LTD, Sydney, NSW Australia
  • György Biró, Budapest, Hungary
  • Anna Blázovics, Semmelweis University, Budapest, Hungary
  • Francesco Capozzi, University of Bologna, Bologna, Italy
  • Marina Carcea, Research Centre for Food and Nutrition, Council for Agricultural Research and Economics Rome, Italy
  • Zsuzsanna Cserhalmi, Budapest, Hungary
  • Marco Dalla Rosa, University of Bologna, Bologna, Italy
  • István Dalmadi, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
  • Katarina Demnerova, University of Chemistry and Technology, Prague, Czech Republic
  • Mária Dobozi King, Texas A&M University, Texas, USA
  • Muying Du, Southwest University in Chongqing, Chongqing, China
  • Sedef Nehir El, Ege University, Izmir, Turkey
  • Søren Balling Engelsen, University of Copenhagen, Copenhagen, Denmark
  • Éva Gelencsér, Budapest, Hungary
  • Vicente Manuel Gómez-López, Universidad Católica San Antonio de Murcia, Murcia, Spain
  • Jovica Hardi, University of Osijek, Osijek, Croatia
  • Hongju He, Henan Institute of Science and Technology, Xinxiang, China
  • Károly Héberger, Research Centre for Natural Sciences, ELKH, Budapest, Hungary
  • Nebojsa Ilić, University of Novi Sad, Novi Sad, Serbia
  • Dietrich Knorr, Technische Universität Berlin, Berlin, Germany
  • Hamit Köksel, Hacettepe University, Ankara, Turkey
  • Katia Liburdi, Tuscia University, Viterbo, Italy
  • Meinolf Lindhauer, Max Rubner Institute, Detmold, Germany
  • Min-Tze Liong, Universiti Sains Malaysia, Penang, Malaysia
  • Marena Manley, Stellenbosch University, Stellenbosch, South Africa
  • Miklós Mézes, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary
  • Áron Németh, Budapest University of Technology and Economics, Budapest, Hungary
  • Perry Ng, Michigan State University,  Michigan, USA
  • Quang Duc Nguyen, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
  • Laura Nyström, ETH Zürich, Switzerland
  • Lola Perez, University of Cordoba, Cordoba, Spain
  • Vieno Piironen, University of Helsinki, Finland
  • Alessandra Pino, University of Catania, Catania, Italy
  • Mojmir Rychtera, University of Chemistry and Technology, Prague, Czech Republic
  • Katharina Scherf, Technical University, Munich, Germany
  • Regine Schönlechner, University of Natural Resources and Life Sciences, Vienna, Austria
  • Arun Kumar Sharma, Department of Atomic Energy, Delhi, India
  • András Szarka, Budapest University of Technology and Economics, Budapest, Hungary
  • Mária Szeitzné Szabó, Budapest, Hungary
  • Sándor Tömösközi, Budapest University of Technology and Economics, Budapest, Hungary
  • László Varga, Széchenyi István University, Mosonmagyaróvár, Hungary
  • Rimantas Venskutonis, Kaunas University of Technology, Kaunas, Lithuania
  • Barbara Wróblewska, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olsztyn, Poland

 

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Acta Alimentaria
Language English
Size B5
Year of
Foundation		 1972
Volumes
per Year		 1
Issues
per Year		 4
Founder Magyar Tudományos Akadémia    
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0139-3006 (Print)
ISSN 1588-2535 (Online)

 

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