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  • 1 Bhabha Atomic Research Center Molecular Biology Division Trombay, Mumbai 400085 India
  • | 2 Bhabha Atomic Research Center Nuclear Agriculture and Biotechnology Division Trombay, Mumbai 400085 India
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The dwarfing genes Rht-B1b and Rht-D1b were incorporated into Indian bread wheats during the “green revolution”. This led to a dramatic increase in yield as well as production of wheat. Although the presence of dwarfing genes is important, their status among the released wheat varieties is generally not known. We present an analysis of the two dwarfing genes using the PCR based “perfect markers” in bread wheat ( Triticum aestivum L. ) varieties, for the first time in 45 Indian, and some non-Indian wheats. Most of the varieties analyzed had either Rht-B1b or Rht-D1b or both. The results obtained for the varieties with known Rht status validated the perfect markers. Analysis of a F 2 population segregating for the dwarfing genes also confirmed the utility of these markers in genetic analysis. Analysis among some of the varieties for the GA response phenotype is also presented. The results showed that the “perfect markers” are robust enough to be used among Indian varieties.

  • Bhagwat, S.G. and Bhatia, C.R. 1994. Quantitative differences in the Gibberellins induced Alpha-amylase activity from aleurone layers of tall and semi-dwarf wheat cultivars. Cereal Res. Com. 22: 1–2, 129–134.

    Bhatia C.R. , 'Quantitative differences in the Gibberellins induced Alpha-amylase activity from aleurone layers of tall and semi-dwarf wheat cultivars ' (1994 ) 22 Cereal Res. Com. : 1 -2.

    • Search Google Scholar
  • Borner, A., Roder, M. and Korzun, V. 1997. Comparative molecular mapping of GA insensitive Rht loci on chromosomes 4B and 4D of common wheat ( Triticum aestivum L.). Theor. Appl. Genet. 95: 1133–1137.

    Korzun V. , 'Comparative molecular mapping of GA insensitive Rht loci on chromosomes 4B and 4D of common wheat (Triticum aestivum L.) ' (1997 ) 95 Theor. Appl. Genet. : 1133 -1137.

    • Search Google Scholar
  • Ellis, M.H., Spielmeyer, W., Gale, K.R., Rebetzke, G.J. and Richards, R.A. 2002. “Perfect markers” for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor. Appl. Genet. 105: 1038–1042.

    Richards R.A. , '“Perfect markers” for the Rht-B1b and Rht-D1b dwarfing genes in wheat ' (2002 ) 105 Theor. Appl. Genet. : 1038 -1042.

    • Search Google Scholar
  • Gale, M.D. and Gregory, R.S. 1977. A rapid method for early generation selection of dwarf varieties in wheat. Euphytica 26: 733–739.

    Gregory R.S. , 'A rapid method for early generation selection of dwarf varieties in wheat ' (1977 ) 26 Euphytica : 733 -739.

    • Search Google Scholar
  • Gale, M.D. and Marshall, G.A. 1975. The nature and genetic control of gibberellin insensitivity in dwarf wheat grain. Heredity 35: 55–65.

    Marshall G.A. , 'The nature and genetic control of gibberellin insensitivity in dwarf wheat grain ' (1975 ) 35 Heredity : 55 -65.

    • Search Google Scholar
  • Gale, M.D. and Marshall, G.A. 1976. The chromosomal location of GAI and Rht 1 genes for gibberellin insensitivity and semi-dwarfism in a derivative of Norin 10 wheat. Heredity 37: 283–289.

    Marshall G.A. , 'The chromosomal location of GAI and Rht 1 genes for gibberellin insensitivity and semi-dwarfism in a derivative of Norin 10 wheat ' (1976 ) 37 Heredity : 283 -289.

    • Search Google Scholar
  • Gale, M.D., Marshall, G.A. and Rao, M.V. 1981. A classification of the Norin 10 and Tom Thumb dwarfing genes in British, Mexican, Indian and other hexaploid bread wheat varieties. Euphytica 30: 355–361.

    Rao M.V. , 'A classification of the Norin 10 and Tom Thumb dwarfing genes in British, Mexican, Indian and other hexaploid bread wheat varieties ' (1981 ) 30 Euphytica : 355 -361.

    • Search Google Scholar
  • Hedden, Peter. 2003. The genes of the Green Revolution. Trends in Genetics. 19: 1

    Hedden Peter. , 'The genes of the Green Revolution ' (2003 ) 19 Trends in Genetics : 1 -.

  • Mahajan, B.K. 1989. Methods in Biostatistics. Jaypee Brothers, New Delhi.

    Mahajan B.K. , '', in Methods in Biostatistics , (1989 ) -.

  • McVittie, J.A., Gale, M.D. and Marshall, G.A. 1978. The intra-chromosomal mapping of the Norin 10 and Tom Thumb dwarfing genes. Heredity 40: 67–70.

    Marshall G.A. , 'The intra-chromosomal mapping of the Norin 10 and Tom Thumb dwarfing genes ' (1978 ) 40 Heredity : 67 -70.

    • Search Google Scholar
  • Nalini, E., Bhagwat, S.G. and Jawali, N. 2004. A simple method for isolation of DNA from plants suitable for long term storage and DNA marker analysis. BARC Newsletter, 249: 208–214.

    Jawali N. , 'A simple method for isolation of DNA from plants suitable for long term storage and DNA marker analysis ' (2004 ) 249 BARC Newsletter : 208 -214.

    • Search Google Scholar
  • Peng, J., Richards, D.E., Hartley, N.M., Murphy, G.P., Devos, K.M., Flintham, J.E., Beales, J., Fish, L.J., Worland, A.J., Pelica, F., Duralalagaraja Sudhakar, Christou, P., Snape, J.W., Gale, M.D. and Harberd, N.P. 1999. ’Green revolution’ genes encode mutant gibberellin response modulators. Nature 400: 256–261.

    Harberd N.P. , '’Green revolution’ genes encode mutant gibberellin response modulators ' (1999 ) 400 Nature : 256 -261.

    • Search Google Scholar
  • Prabhu, K.V., Gupta, S.K., Charpe, A., Koul, S., Cherukuri, D.P., Dhaliwal, H.S., Vikal, Y., Chhuneja, P. and Haq, Q.M.R. 2003. Molecular markers detect redundancy and miss-identity in genetic stocks with alien Leaf Rust resistance genes Lr32 and Lr28 in bread wheat. J. Plant Biochem. & Biotechnol., 12: 123–129.

    Haq Q.M.R. , 'Molecular markers detect redundancy and miss-identity in genetic stocks with alien Leaf Rust resistance genes Lr32 and Lr28 in bread wheat ' (2003 ) 12 J. Plant Biochem. & Biotechnol. : 123 -129.

    • Search Google Scholar
  • Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

    Maniatis T. , '', in Molecular cloning: a laboratory manual , (1989 ) -.

  • Singh, R.B. and Kulshrestha, V.P. (1996) 50 years of crop science research in India. (ICAR, New Delhi). Wheat. 219–249.

    Kulshrestha V.P. , '', in 50 years of crop science research in India , (1996 ) -.

  • Singh, S.S. and Singh, B.B. 2001. IARI Wheats for Evergreen Revolution. (IARI, New Delhi). P. 34.

    Singh B.B. , '', in IARI Wheats for Evergreen Revolution , (2001 ) -.

  • Sourdille, P., Charmet, G., Trottet, M., Tixier, M.H., Boeuf, C., Negre, S., Barloy, D. and Bernard, M. 1998. Linkage between RFLP molecular markers and the dwarfing genes Rht-B1b and Rht-D1b in wheat. Hereditas 128: 41–46.

    Bernard M. , 'Linkage between RFLP molecular markers and the dwarfing genes Rht-B1b and Rht-D1b in wheat ' (1998 ) 128 Hereditas : 41 -46.

    • Search Google Scholar

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Cereal Research Communications
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