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  • 1 University of Rajasthan Department of Botany Jaipur India 302004
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The effect of potassium dihydrogen phosphate (KH 2 PO 4 ) on callus induction and plant regeneration from callus cultures of indica rice ( Oryza sativa cultivar Pusa Basmati-1) was investigated. Callus was induced on Murashige and Skoog (MS) medium supplemented with 11.31 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and subsequent plant regeneration was obtained on MS medium containing 2.68 μM α-naphthaleneacetic acid (NAA) and 8.87 μM 6-benzylaminopurine (BAP). Both the induction and the regeneration media were supplemented with different levels of KH 2 PO 4 (0 to 12.50 mM). The level of KH 2 PO 4 in the induction medium influenced the percent water content of the callus. KH 2 PO 4 was found to be essential for plant regeneration as no shoot regeneration occurred in the absence of KH 2 PO 4 . Highest number of shoots per explant was obtained when KH 2 PO 4 level in the callus induction and plant regeneration medium was 0.625 mM (half of MS level) and 1.25 mM (normal MS level) respectively. By making amendments and modifications of the KH 2 PO 4 level in the induction medium, the regeneration increased 2.5 folds. Almost similar regeneration frequency was observed from callus induced on lower levels of KH 2 PO 4 . Regenerated shoots were rooted on MS medium supplemented with 2.68 μM NAA. Rooted plantlets were transferred to soil where they survived and set seeds.

  • Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technol 4:1087–1090

    Thompson J.A. , 'Efficient plant regeneration from rice protoplasts through somatic embryogenesis ' (1986 ) 4 Bio/Technol : 1087 -1090.

    • Search Google Scholar
  • Chen TH, Lam L, Chen SH (1985) Somatic embryogenesis and plant regeneration from cultured young inflorescences of Oryza sativa L. (rice). Plant Cell Tiss Org Cult 4:51–54

    Chen S.H. , 'Somatic embryogenesis and plant regeneration from cultured young inflorescences of Oryza sativa L. (rice) ' (1985 ) 4 Plant Cell Tiss Org Cult : 51 -54.

    • Search Google Scholar
  • Dahleen LS (1995) Improved plant regeneration from barley callus cultures by improved copper levels. Plant Cell Tiss Organ Cult 43:267–269

    Dahleen L.S. , 'Improved plant regeneration from barley callus cultures by improved copper levels ' (1995 ) 43 Plant Cell Tiss Organ Cult : 267 -269.

    • Search Google Scholar
  • Dahleen LS, Bregitzer P (2002) An improved media system for high regeneration rates from barley immature embryo-derived callus cultures of commercial cultivars. Crop Sci 42:934–938

    Bregitzer P. , 'An improved media system for high regeneration rates from barley immature embryo-derived callus cultures of commercial cultivars ' (2002 ) 42 Crop Sci : 934 -938.

    • Search Google Scholar
  • Grimes HD, Hodges TK (1990) The inorganic NO 3 : NH 4 + ratio influences plant regeneration and auxin sensitivity in primary callus derived from immature embryos of indica rice ( Oryza sativa L.). J Plant Physiol 136:362–367

    Hodges T.K. , 'The inorganic NO3−: NH4+ ratio influences plant regeneration and auxin sensitivity in primary callus derived from immature embryos of indica rice (Oryza sativa L.) ' (1990 ) 136 J Plant Physiol : 362 -367.

    • Search Google Scholar
  • He DG, Yang YM, Scott KJ (1989) The effect of macronutrients in the induction of embryogenic callus from immature embryos of wheat ( Triticum aestivum L.). Plant Sci 64:251–258

    Scott K.J. , 'The effect of macronutrients in the induction of embryogenic callus from immature embryos of wheat (Triticum aestivum L.) ' (1989 ) 64 Plant Sci : 251 -258.

    • Search Google Scholar
  • Jain RK, Jain S, Wu R (1996) Stimulatory effect of water stress on plant regeneration of aromatic indica rice varieties. Plant Cell Rep 15:1449–1459

    Wu R. , 'Stimulatory effect of water stress on plant regeneration of aromatic indica rice varieties ' (1996 ) 15 Plant Cell Rep : 1449 -1459.

    • Search Google Scholar
  • Khanna HK, Raina SK (1997) Enhanced plant regeneration in basmati rice ( Oryza sativa L. cv. Karnal Local) embryo calli through modification of NO 3 + NH4 + concentrations. J Biochem Biotech 6:75–80

    Raina S.K. , 'Enhanced plant regeneration in basmati rice (Oryza sativa L. cv. Karnal Local) embryo calli through modification of NO3− + NH4+ concentrations ' (1997 ) 6 J Biochem Biotech : 75 -80.

    • Search Google Scholar
  • Khanna HK, Raina SK (1998) Genotype X culture media interaction effects on regeneration response of three indica rice cultivars. Plant Cell Tiss Org Cult 52:145–153

    Raina S.K. , 'Genotype X culture media interaction effects on regeneration response of three indica rice cultivars ' (1998 ) 52 Plant Cell Tiss Org Cult : 145 -153.

    • Search Google Scholar
  • Koetje DS, Grimes HD, Wang YC, Hodges TK (1989) Regeneration of indica rice ( Oryza sativa L.) from primary callus derived from immature embryos. J Plant Physiol 135:184–190

    Hodges T.K. , 'Regeneration of indica rice (Oryza sativa L.) from primary callus derived from immature embryos ' (1989 ) 135 J Plant Physiol : 184 -190.

    • Search Google Scholar
  • Kothari SL, Agarwal K, Kumar S (2004) Inorganic nutrient manipulation for highly improved in vitro plant regeneration in finger millet — Eleusine coracana (L.) Gaertn. In Vitro Cell Dev Biol (in press)

  • Lee K, Jeon H, Kim M (2002) Optimization of a mature embryo based in vitro culture system for high frequency somatic embryogenic callus induction and plant regeneration from japonica rice cultivars. Plant Cell Tiss Org Cult 17:237–244

    Kim M. , 'Optimization of a mature embryo based in vitro culture system for high frequency somatic embryogenic callus induction and plant regeneration from japonica rice cultivars ' (2002 ) 17 Plant Cell Tiss Org Cult : 237 -244.

    • Search Google Scholar
  • Leifert C, Murphy KP, Lumsden PJ (1995) Mineral and carbohydrate nutrition of plant cell and tissue cultures. Crit Rev Plant Sci 14(2):83–109

    Lumsden P.J. , 'Mineral and carbohydrate nutrition of plant cell and tissue cultures ' (1995 ) 14 Crit Rev Plant Sci : 83 -109.

    • Search Google Scholar
  • Loneragan JF, Asher CJ (1982) Response of plants to phosphate concentration in solution culture: II. Rate of phosphate absorption and its relation to growth. Soil Sci 103:311–318.

    Asher C.J. , 'Response of plants to phosphate concentration in solution culture: II. Rate of phosphate absorption and its relation to growth ' (1982 ) 103 Soil Sci : 311 -318.

    • Search Google Scholar
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Skoog F. , 'A revised medium for rapid growth and bioassays with tobacco tissue cultures ' (1962 ) 15 Physiol Plant : 473 -497.

    • Search Google Scholar
  • Nirwan RS, Kothari SL (2003) High copper levels improve callus induction and plant regeneration in Sorghum bicolor (L.) Moench. In Vitro Cell Dev Bio 2:165–168

    Kothari S.L. , 'High copper levels improve callus induction and plant regeneration in Sorghum bicolor (L.) Moench ' (2003 ) 2 In Vitro Cell Dev Bio : 165 -168.

    • Search Google Scholar
  • Nishi T, Yamada Y, Takahashi E (1968) Organ differentiation and plant restoration in rice callus. Nature 219:508–509

    Takahashi E. , 'Organ differentiation and plant restoration in rice callus ' (1968 ) 219 Nature : 508 -509.

    • Search Google Scholar
  • Oinam GS, Kothari SL (1995) Totipotency of coleoptile tissue in indica rice ( Oryza sativa L. cv. CH 1039). Plant Cell Rep 14:245–248

    Kothari S.L. , 'Totipotency of coleoptile tissue in indica rice (Oryza sativa L. cv. CH 1039) ' (1995 ) 14 Plant Cell Rep : 245 -248.

    • Search Google Scholar
  • Poddar K, Vishoni RK, Kothari SL (1997) Plant regeneration from embryogenic callus of finger millet Eieusine coracana (L.) Gaertn. on high concentration of NH 4 NO 3 as a replacement of NAA in the medium. Plant Sci 129:101–106

    Kothari S.L. , 'Plant regeneration from embryogenic callus of finger millet Eieusine coracana (L.) Gaertn. on high concentration of NH4NO3 as a replacement of NAA in the medium ' (1997 ) 129 Plant Sci : 101 -106.

    • Search Google Scholar
  • Preece JE (1995) Can nutrient salts partially substitute for plant growth regulators? Plant Tiss Cult Biotech 1:26–37

    Preece J.E. , 'Can nutrient salts partially substitute for plant growth regulators? ' (1995 ) 1 Plant Tiss Cult Biotech : 26 -37.

    • Search Google Scholar
  • Purnhauser L (1991) Stimulation of shoot and root regeneration in wheat Triticum aestivum callus cultures by copper. Cereal Res Comm 19:419–423

    Purnhauser L. , 'Stimulation of shoot and root regeneration in wheat Triticum aestivum callus cultures by copper ' (1991 ) 19 Cereal Res Comm : 419 -423.

    • Search Google Scholar
  • Purnhauser L, Gyulia G (1993) Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue cultures. Plant Cell Tiss Organ Cult 35:131–139

    Gyulia G. , 'Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue cultures ' (1993 ) 35 Plant Cell Tiss Organ Cult : 131 -139.

    • Search Google Scholar
  • Ramage CM, Williams RR (2002) Mineral nutrition and plant morphogenesis. In Vitro Cell Dev Biol 38:116–124

    Williams R.R. , 'Mineral nutrition and plant morphogenesis ' (2002 ) 38 In Vitro Cell Dev Biol : 116 -124.

    • Search Google Scholar
  • Rance IM, Tian W, Mathews H, Kochko A, Beachy RN, Fauquet C (1994) Partial desiccation of mature embryo-derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice. Plant Cell Rep 13:647–651

    Fauquet C. , 'Partial desiccation of mature embryo-derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice ' (1994 ) 13 Plant Cell Rep : 647 -651.

    • Search Google Scholar
  • Raval M, Chattoo BB (1993) Role of media constituents and proline in callus growth, somatic embryogenesis and regeneration of Oryza sativa cv indica. Ind J Exp Bio 31:600–603

    Chattoo B.B. , 'Role of media constituents and proline in callus growth, somatic embryogenesis and regeneration of Oryza sativa cv indica ' (1993 ) 31 Ind J Exp Bio : 600 -603.

    • Search Google Scholar
  • Sahrawat AK, Chand S (2002) Influence of ammonium nitrate on plant regeneration in indica rice ( Oryza sativa Linn.). Ind J Biotech 1:367–371

    Chand S. , 'Influence of ammonium nitrate on plant regeneration in indica rice (Oryza sativa Linn.) ' (2002 ) 1 Ind J Biotech : 367 -371.

    • Search Google Scholar
  • Sahrawat AK, Chand S (1999) Stimulatory effect of copper on plant regeneration in indica rice. J Plant Physiol 154:517–522

    Chand S. , 'Stimulatory effect of copper on plant regeneration in indica rice ' (1999 ) 154 J Plant Physiol : 517 -522.

    • Search Google Scholar
  • Sticklen MB (1991) Direct somatic embryogenesis and fertile plants from rice root cultures. J Plant Physiol 138:577–580

    Sticklen M.B. , 'Direct somatic embryogenesis and fertile plants from rice root cultures ' (1991 ) 138 J Plant Physiol : 577 -580.

    • Search Google Scholar
  • Wernicke W, Brettel R, Wakizuma T, Potrykus I (1981) Adventitious embryoid and root formation from rice leaves. Z Planzenphysiol 103:361–365

    Potrykus I. , 'Adventitious embryoid and root formation from rice leaves ' (1981 ) 103 Z Planzenphysiol : 361 -365.

    • Search Google Scholar
  • Zhao J, Zhou C, Yang HY (1999) In vitro development of early proembryos and plant regeneration via microculture in Oryza sativa . Plant Cell Tiss Org Cult 55:167–174

    Yang H.Y. , 'In vitro development of early proembryos and plant regeneration via microculture in Oryza sativa ' (1999 ) 55 Plant Cell Tiss Org Cult : 167 -174.

    • Search Google Scholar

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Cereal Research Communications
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