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  • 1 Experimental Institute for Cereal Research Via Cassia 176 00191 Rome Italy
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In this study elevated temperature effects on protein quality parameters and in particular on protein sulfhydryl-disulfide contents were assessed. Four Italian durum wheat cultivars which differed for the qualitative trait changes produced by heat stress were employed. During grain filling two different thermal regimes were imposed on the plants grown in a typical area of durum wheat cultivation (Southern Italy). Results confirmed significant effects of high temperatures on the quality of products in the durum wheat chain and indicated that elevated temperatures determined changes on protein redox status, particularly evident on S-S bond content. For most of the examined traits significant cultivar x treatment interactions were also evidenced.

  • Altenbach S.B., Kothari K.M. and Lieu D. 2002. Environmental conditions during wheat grain development alter temporal regulation of major gluten protein genes. Cereal Chem. 79: 279–285.

    Lieu D. , 'Environmental conditions during wheat grain development alter temporal regulation of major gluten protein genes ' (2002 ) 79 Cereal Chem. : 279 -285.

    • Search Google Scholar
  • Altenbach S.B., DuPont F.M., Kothari K.M., Chan R., Johnson E.L. and Lieu D. 2003. Temperature, water and fertilizer influence the timing of key events during grain development in a US spring wheat. J. Cereal Sci. 37: 9–20.

    Lieu D. , 'Temperature, water and fertilizer influence the timing of key events during grain development in a US spring wheat ' (2003 ) 37 J. Cereal Sci. : 9 -20.

    • Search Google Scholar
  • American Association of Cereal Chemists. 1969. Approved Methods. Method 38–12. AACC, St. Paul, Mn.

    '', in Method , (1969 ) -.

  • American Association of Cereal Chemists. 1969. Approved Methods. Method 56–70. AACC, St. Paul, Mn.

    '', in Method , (1969 ) -.

  • Anderson A.K. and Ng P.K.W. 2000. Changes in disulfide and sulfhydryl contents and electrophoretic patterns of extruded wheat flour proteins. Cereal Chem. 77: 354–359.

    Ng P.K.W. , 'Changes in disulfide and sulfhydryl contents and electrophoretic patterns of extruded wheat flour proteins ' (2000 ) 77 Cereal Chem. : 354 -359.

    • Search Google Scholar
  • Blumenthal C., Wrigley C.W., Batey I.L., and Barlow E.W.R. 1994. The heat-shock response relevant to molecular and structural changes in wheat yield and quality. Aust. J. Plant Physiol. 21:901–909.

    Barlow E.W.R. , 'The heat-shock response relevant to molecular and structural changes in wheat yield and quality ' (1994 ) 21 Aust. J. Plant Physiol. : 901 -909.

    • Search Google Scholar
  • Blumenthal C., Bekes F., Gras P.W., Barlow E.W.R. and Wrigley C.W. 1995. Identification of wheat genotypes tolerant to the effects of heat stress on grain quality. Cereal Chem. 72: 539–544.

    Wrigley C.W. , 'Identification of wheat genotypes tolerant to the effects of heat stress on grain quality ' (1995 ) 72 Cereal Chem. : 539 -544.

    • Search Google Scholar
  • Blumenthal C., Stone P.J., Gras P.W., Bekes F., Clarke B., Barlow E.W.R., Appels R. and Wrigley C.W. 1998. Heat-shock protein 70 and dough-quality changes resulting from heat stress during grain filling in wheat. Cereal Chem. 75: 43–50.

    Wrigley C.W. , 'Heat-shock protein 70 and dough-quality changes resulting from heat stress during grain filling in wheat ' (1998 ) 75 Cereal Chem. : 43 -50.

    • Search Google Scholar
  • Borghi B., Corbellini M., Ciaffi M., Lafiandra D., De Stefanis E., Sgrulletta D., Boggini G., Di Fonzo N. 1995. Effect of heat shock during grain filling on grain quality of bread and durum wheats. Aust. J. Agric. Res. 46: 1365–1380.

    Fonzo N. , 'Effect of heat shock during grain filling on grain quality of bread and durum wheats ' (1995 ) 46 Aust. J. Agric. Res. : 1365 -1380.

    • Search Google Scholar
  • Ciaffi M., Margiotta B., Colaprico G., De Stefanis E., Sgrulletta D., and Lafiandra D. 1995. Effect of high temperatures during grain filling on the amount of insoluble proteins in durum wheat. J. Genet. & Breed. 49: 285–296.

    Lafiandra D. , 'Effect of high temperatures during grain filling on the amount of insoluble proteins in durum wheat ' (1995 ) 49 J. Genet. & Breed. : 285 -296.

    • Search Google Scholar
  • Corbellini M., Canevar M.G., Mazza L., Ciaffi M., Lafiandra D. and Borghi B. 1997. Effect of the duration and intensity of heat shock during grain filling on dry matter and protein accumulation, technological quality and protein composition in bread and durum wheat. Aust. J. Plant Physiol. 24: 245–260.

    Borghi B. , 'Effect of the duration and intensity of heat shock during grain filling on dry matter and protein accumulation, technological quality and protein composition in bread and durum wheat ' (1997 ) 24 Aust. J. Plant Physiol. : 245 -260.

    • Search Google Scholar
  • Daniel C. and Triboi E. 2000. Effects of temperature and nitrogen nutrition on the grain composition of winter wheat: effects on gliadin content and composition. J. Cereal Sci. 32: 45–56.

    Triboi E. , 'Effects of temperature and nitrogen nutrition on the grain composition of winter wheat: effects on gliadin content and composition ' (2000 ) 32 J. Cereal Sci. : 45 -56.

    • Search Google Scholar
  • D’Egidio M.G., De Stefanis E., Fortini S., Galterio G., Nardi S., Sgrulletta D., and Bozzini A. 1982. Standardization of cooking quality analysis in macaroni and pasta products. Cereal Foods World 27: 367–368.

    Bozzini A. , 'Standardization of cooking quality analysis in macaroni and pasta products ' (1982 ) 27 Cereal Foods World : 367 -368.

    • Search Google Scholar
  • De Stefanis E., Sgrulletta D., Corbellini M., Di Fonzo N., Ronga G. 1998. Accumulation of acetic acid-soluble and insoluble proteins in grain of T. durum cultivars grown under heat stress conditions. J. Genet. & Breed. 52: 343–352.

    Ronga G. , 'Accumulation of acetic acid-soluble and insoluble proteins in grain of T. durum cultivars grown under heat stress conditions ' (1998 ) 52 J. Genet. & Breed. : 343 -352.

    • Search Google Scholar
  • De Stefanis E., Sgrulletta D. 1999. NADP-tioredoxina reduttasi e sue implicazioni con la qualità del frumento. Proceedings of Meeting “Materie prime transgeniche, sicurezza alimentare e controllo qualità nell’industria cerealicola”, Campobasso (Italy) October October 7–8, 1999: 143–149.

  • De Stefanis E., Sgrulletta D., De Vita P., Ronga G. 2000. Effetto delle alte temperature durante il “grain filling” sulle caratteristiche tecnologiche e qualitative del frumento duro. Proceedings of Meeting “Prospettive di una moderna cerealicoltura alle porte del terzo millennio”, Foggia (Italy) April 30–2 May, 1998: 167–178.

  • De Stefanis E., Sgrulletta D., De Vita P., Pucciarmati S. 2002. Genetic variability to the effects of heat stress during grain filling on durum wheat quality. Cereal Res. Comm. 30: 117–124.

    Pucciarmati S. , 'Genetic variability to the effects of heat stress during grain filling on durum wheat quality ' (2002 ) 30 Cereal Res. Comm. : 117 -124.

    • Search Google Scholar
  • Dupuis B., Bushuk W. and Sapirstein H.D. 1996. Characterization of acid soluble and insoluble fractions of glutenin of bread wheat. Cereal Chem. 73: 131–135.

    Sapirstein H.D. , 'Characterization of acid soluble and insoluble fractions of glutenin of bread wheat ' (1996 ) 73 Cereal Chem. : 131 -135.

    • Search Google Scholar
  • Guedira M., McCluskey P.J., Mac Ritchie F. and Paulsen G.M. 2002. Composition and quality of wheat grown under different shoot and root temperatures during maturation. Cereal Chem. 79: 397–403.

    Paulsen G.M. , 'Composition and quality of wheat grown under different shoot and root temperatures during maturation ' (2002 ) 79 Cereal Chem. : 397 -403.

    • Search Google Scholar
  • International Association for Cereal Chemistry. 1986. Standard Method of the ICC. Method 121.

  • Keck-Gassenmeier B. and Wieser H. 1996. Disulfide bonds in acetic acid soluble and insoluble glutenin fractions. Proceedings of the 6 th International Gluten Workshop, Sidney September 1–6, 1996: 145–148.

  • Naeem H.A. and Mac Ritchie F. 2003. Heat stress effects on wheat proteins during grain development. Proceedings of the tenth International Wheat Genetics Symposium, Paestum (Italy) September 1–6, 2003: 455–458.

  • Rhazi L., Cazalis R., Aussenac T. 2003. Sulfhydryl-disulfide changes in storage proteins of developing wheat grain: influence on the SDS-unextractable glutenin polymer formation. J. Cereal Sci. 38: 3–13.

    Aussenac T. , 'Sulfhydryl-disulfide changes in storage proteins of developing wheat grain: influence on the SDS-unextractable glutenin polymer formation ' (2003 ) 38 J. Cereal Sci. : 3 -13.

    • Search Google Scholar
  • Sgrulletta D. and De Stefanis E. 1989. Relationship between pasta cooking quality and acetic acid-insoluble protein of semolina. J. Cereal Sci. 9: 217–220.

    Stefanis E. , 'Relationship between pasta cooking quality and acetic acid-insoluble protein of semolina ' (1989 ) 9 J. Cereal Sci. : 217 -220.

    • Search Google Scholar
  • Shi Y.C., Seib P.A. and Bernardin J.E. 1994. Effects of temperature during grain-filling on starches from six wheat cultivars. Cereal Chem. 71: 369–383.

    Bernardin J.E. , 'Effects of temperature during grain-filling on starches from six wheat cultivars ' (1994 ) 71 Cereal Chem. : 369 -383.

    • Search Google Scholar
  • Skylas D.J., Cordwell S.J., Hains P.G., Larsen M.R., Basseal D.J., Walsh B.J., Blumenthal C., Rathmell W., Copeland L. and Wrigley C.W. 2002. Heat shock of wheat during grain filling: proteins associated with heat-tolerance. J. Cereal Sci. 35: 175–188.

    Wrigley C.W. , 'Heat shock of wheat during grain filling: proteins associated with heat-tolerance ' (2002 ) 35 J. Cereal Sci. : 175 -188.

    • Search Google Scholar
  • UNI (Ente Nazionale Italiano di Unificazione) 1994. Cereali: determinazione della massa per ettolitro o peso ettolitrico. Metodo 10281:1–9.

    'Cereali: determinazione della massa per ettolitro o peso ettolitrico ' (1994 ) 10281 Metodo : 1 -9.

    • Search Google Scholar