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  • 1 Agricultural Research Institute of the Hungarian Academy of Sciences P.O. Box 19 H-2462 Martonvásár Hungary
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The aims of the study were the optimisation of genomic in situ hybridization (GISH) and the use of a GAA satellite sequences as a fluorescence in situ hybridization (FISH) probe to discriminate Aegilops biuncialis and wheat chromosomes. The application of genomic DNA from the diploid progenitors, Ae. umbellulata and Ae. comosa , as a probe with a blocking ratio of 1:200 resulted in the clear visualization of Ae. biuncialis chromosomes. Based on the strong hybridization signals of the PCR-amplified GAA satellite sequences it was possible to identify chromosomes 1U, 2U, 4U and 5U of Ae. umbellulata . The improved GISH and GAA idiogram presented here allow the Ae. biuncialis chromatin to be traced in more detail during chromosome-mediated gene transfer to wheat.

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Cereal Research Communications
Language English
Size B5
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
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Address
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Springer Nature Switzerland AG
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ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)