View More View Less
  • 1 Agricultural University of Cracow Department of Plant Breeding and Seed Science Łobzowska 24 31-140 Kraków Poland
Restricted access

The aim of the study was to determine the effect of various 2,4-D concentrations on the androgenic response and plant development in anther culture of barley. Anthers were either plated directly on the FHG medium supplemented with 20 mg dm −3 of 2,4-D, or pretreated with 0.7 mol dm −3 mannitol and plated on an induction medium containing various concentrations of 2,4-D: 0.2, 2.0, or 20 mg dm −3 . The concentration of 20 mg dm −3 was used for 3 days only; subsequently, anthers were transferred onto the medium containing 0.2 mg dm −3 of 2,4-D. It was shown that osmotic stress cannot be replaced with the high concentration of 2,4-D. The most numerous embryos, embryogenic calli, and plants (including green plants) were obtained with 0.7mol dm −3 mannitol pretreatment and 0.2 mg dm −3 of 2,4-D in the induction medium. The auxin concentration also affected the dynamics of embryogenic structure formation and plant development from embryogenic structures. The concentrations higher than 0.2 mg dm −3 slowed down embryogenic structure formation and plant development, and they increased the proportion of calli as compared to embryos in plant regeneration.

  • Cai Q., Szarejko I., Polok K., Maluszyński M. (1992). The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breeding 109: 218–226.

    Maluszyński M. , 'The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture ' (1992 ) 109 Plant Breeding : 218 -226.

    • Search Google Scholar
  • Hoekstra S., van Bergen S., van Brouwershaven I. R., Schilperoort R. A. Heidekamp F. (1996). The interaction of 2,4-D application and mannitol pretreatment in anther and microspore culture of Hordeum vulgare L. cv. Igri. J. Plant Physiol. 148: 696–700.

    Heidekamp F. , 'The interaction of 2,4-D application and mannitol pretreatment in anther and microspore culture of Hordeum vulgare L. cv. Igri ' (1996 ) 148 J. Plant Physiol. : 696 -700.

    • Search Google Scholar
  • Hu H. (1997). In vitro induced haploids in wheat. In: In vitro Haploid Production in Higher Plants, vol. 4 (S. M. Jain, S. K. Sopory, R. E. Veilleux, eds.). Kluwer Acad. Publishers, Dordrecht: 73–97.

    Hu H. , '', in In vitro Haploid Production in Higher Plants, vol. 4 , (1997 ) -.

  • Indrianto A., Heberle-Bors E., Touraev A. (1999). Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores. Plant Sci. 143: 71–79.

    Touraev A. , 'Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores ' (1999 ) 143 Plant Sci. : 71 -79.

    • Search Google Scholar
  • Jähne-Gärtner A., Lörz H. (1996). In vitro regeneration systems of barley ( Hordeum vulgare L.). Plant Tissue Culture and Biotechnology 2(1): 11–23.

    Lörz H. , 'In vitro regeneration systems of barley (Hordeum vulgare L.) ' (1996 ) 2 Plant Tissue Culture and Biotechnology : 11 -23.

    • Search Google Scholar
  • Kasha K.J., Simion E., Oro R. Yao Q. A., Hu T. C., Carlson A. R. (2001). An improved in vitro technique for isolated microspore culture of barley. Euphytica 120: 379–385.

    Carlson A. R. , 'An improved in vitro technique for isolated microspore culture of barley ' (2001 ) 120 Euphytica : 379 -385.

    • Search Google Scholar
  • Kruczkowska H., Pawłowska H., Skucińska B. (2002). Influence of anther pretreatment on the efficiency of androgenesis in barley. J. Appl. Genet. 43: 287–296.

    Skucińska B. , 'Influence of anther pretreatment on the efficiency of androgenesis in barley ' (2002 ) 43 J. Appl. Genet. : 287 -296.

    • Search Google Scholar
  • Kuhlmann U., Foroughi-Wehr B. (1989). Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep. 8: 78–81.

    Foroughi-Wehr B. , 'Production of doubled haploid lines in frequencies sufficient for barley breeding programs ' (1989 ) 8 Plant Cell Rep. : 78 -81.

    • Search Google Scholar
  • Li H., Devaux P. (2001). Enhancement of microspore culture efficiency of recalcitrant barley genotypes. Plant Cell Rep. 20: 475–481.

    Devaux P. , 'Enhancement of microspore culture efficiency of recalcitrant barley genotypes ' (2001 ) 20 Plant Cell Rep. : 475 -481.

    • Search Google Scholar
  • Marsolais A. A., Kasha K. J. (1985). Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium. Can. J. Bot. 63: 2209–2218.

    Kasha K. J. , 'Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium ' (1985 ) 63 Can. J. Bot. : 2209 -2218.

    • Search Google Scholar
  • Ohnoutková L., Novotný J., Müllerová E., Vagera J., Kučera L. (2000). Is a cold pretreatment really needed for induction of in vitro androgenesis in barley and wheat? In: Biotechnological Approaches for Utilization of Gametic Cells (B. Bohanec, ed.). COST 824, Bled, Slovenia 1–5 July 2000: 33–37.

  • Powell W. (1988). Diallel analysis of barley anther culture response. Genome 30: 152–157.

    Powell W. , 'Diallel analysis of barley anther culture response ' (1988 ) 30 Genome : 152 -157.

    • Search Google Scholar
  • Szarejko I. (1991). Kultury pylnikowe jęczmienia ( Hordeum vulgare L.) i ocena genetyczna podwojonych haploidów (Anther culture of Hordeum vulgare L. and genetic evaluation of microspore derived doubled haploids). Prace Naukowe UŚ 1232, Katowice: 1–124.

  • Szarejko I. (2003). Anther culture for doubled haploid production in barley ( Hordeum vulgare L.). In: Maluszynski M., Kasha K.J., Forster B.P., Szarejko I. (eds), Doubled Haploid Production in Crop Plants. Kluwer Acad. Publ., the Netherlands: 35–42.

    Szarejko I. , '', in Doubled Haploid Production in Crop Plants , (2003 ) -.