View More View Less
  • 1 Sichuan Agricultural University Triticeae Research Institute Dujiangyan 611830 China
Restricted access

Xinjiang rice wheat ( Triticum petropavlovskyi Udacz. et Migush, 2n=6x=42, AABBDD) is one of the endemic Chinese wheats, only distributing in Xinjiang and Xizang (Tibet), China. A novel high-molecular-weight (HMW) glutenin subunit gene 1Dx2.1 was isolated and characterized from Xinjiang rice wheat accession Daomai2. The complete open reading frame (ORF) of 1Dx2.1 is 2508 bp, encoding 836 amino acids. The primary structure of 1Dx2.1 consists of three distinct domains, a non-repetitive N-terminal domain with 89 residues, a non-repetitive C-terminal domain with 42 residues and a large central repetitive domain with 684 residues. In the N-terminal of 1Dx2.1, there is an R (arginine) at position 75, whereas there is a Q (glutamine) in other known x-type subunits. Four cysteine residues are observed in 1Dx2.1 with three in the N-terminal region and one in the C-terminal region. The number and distribution of cysteines in 1Dx2.1 are identical to those in x-type subunits except for 1Dx5, which possesses an extra cysteine residue. Differences between the repetitive domain of 1Dx2.1 and those of known HMW subunits resulted from substitutions, insertions or/and deletions involving single or more amino acid residues. The phylogenetic tree, which was constructed on the basis of amino acid sequences, and indicated that 1Dx2.1 was highly related to 1Dx2.1 t , then to 1Dx2 and 1Dx5.

  • Altschul, S., W. Gish, W. Miller, E. Myers, D. Lipman. 1990. Basic local alignment search tool. J. Mol Biol. 215:403–410.

    Lipman D. , 'Basic local alignment search tool ' (1990 ) 215 J. Mol Biol. : 403 -410.

  • Anderson, O.D., F.C. Greene. 1989. The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor. Appl. Genet. 77:689–700.

    Greene F.C. , 'The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat ' (1989 ) 77 Theor. Appl. Genet. : 689 -700.

    • Search Google Scholar
  • Anderson, O.D., F.C. Greene, R.E. Yip, N.G. Halford, P.R. Shewry, J.M. Malpica-Romero. 1989. Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res. 17:461–462.

    Malpica-Romero J.M. , 'Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne ' (1989 ) 17 Nucleic Acids Res. : 461 -462.

    • Search Google Scholar
  • Forde, J., J.M. Malpica, N.G. Halford, P.R. Shewry, O.D. Anderson, F.C. Greene, B.J. Miflin. 1985. Nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat ( Triticum aestivum L.). Nucleic Acids Res. 13:6817–6832.

    Miflin B.J. , 'Nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.) ' (1985 ) 13 Nucleic Acids Res. : 6817 -6832.

    • Search Google Scholar
  • Gianibelli, M.C., R.G. Solomon 2003. A Novel y-type High Mr Glutenin Subunit (12.4t) Present in Triticum tauschii . J. Cereal Sci. 37:253–256.

    Solomon R.G. , 'A Novel y-type High Mr Glutenin Subunit (12.4t) Present in Triticum tauschii ' (2003 ) 37 J. Cereal Sci. : 253 -256.

    • Search Google Scholar
  • Gupta, R. B., and F. MacRitchie. 1994. Allelic variation of glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheat. I. Its additive and interaction effects on dough properties. J. Cereal Sci. 19:9–17.

    MacRitchie F. , 'Allelic variation of glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheat. I. Its additive and interaction effects on dough properties ' (1994 ) 19 J. Cereal Sci. : 9 -17.

    • Search Google Scholar
  • Halford, N.G., J. Forde, O.D. Anderson, F.C. Greene, P.R. Shewry. 1987. The nucleotide and deduced amino acid sequence of an HMW glutenin subunit gene from chromosome 1B of bread wheat ( Triticum aestivum L.) and comparison with those of genes from chromosome 1A and 1D. Theor. Appl. Genet. 75:117–126.

    Shewry P.R. , 'The nucleotide and deduced amino acid sequence of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosome 1A and 1D ' (1987 ) 75 Theor. Appl. Genet. : 117 -126.

    • Search Google Scholar
  • Halford, N.G., J. M. Field, H. Blair, P. Urwin, K. Moore, L. Robert, R. Thompson, R. B. Flavell, A.S. Tatham, and P.R. Shewry. 1992. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat ( Triticum aestivum L.) indicates quantitative effects on grain quality. Theory. Appl. Genet. 83:373–378.

    Shewry P.R. , 'Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality ' (1992 ) 83 Theory. Appl. Genet. : 373 -378.

    • Search Google Scholar
  • Liu, Z., Z. Yan, Y. Wan, K. Liu, Y. Zheng, D. Wang. 2003. Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theor. Appl. Genet. 106:1368–1378.

    Wang D. , 'Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species ' (2003 ) 106 Theor. Appl. Genet. : 1368 -1378.

    • Search Google Scholar
  • Mackie, A.M., P.J. Sharp, E.S. Lagudah. 1996. The nucleotide and derived amino acid sequence of a HMW glutenin gene from Triticum. tauschii and comparison with those from the of D genome of bread wheat. J. Cereal Sci. 24:73–78.

    Lagudah E.S. , 'The nucleotide and derived amino acid sequence of a HMW glutenin gene from Triticum. tauschii and comparison with those from the of D genome of bread wheat ' (1996 ) 24 J. Cereal Sci. : 73 -78.

    • Search Google Scholar
  • Nei, N., S. Kumar, 2000. Molecular evolution and phylogenetics. Oxford University Press, UK.

    Kumar S. , '', in Molecular evolution and phylogenetics , (2000 ) -.

  • Payne, P.I., K.G. Corfield, and J.A. Blackman. 1981. Correlation between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J. Sci. Food Agric. 32:51–60.

    Blackman J.A. , 'Correlation between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat ' (1981 ) 32 J. Sci. Food Agric. : 51 -60.

    • Search Google Scholar
  • Payne, P.I. and G.J. Lawrence. 1983. Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1 and Glu-D1 , which code for the high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11:29–35.

    Lawrence G.J. , 'Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1 and Glu-D1, which code for the high-molecular-weight subunits of glutenin in hexaploid wheat ' (1983 ) 11 Cereal Res. Comm. : 29 -35.

    • Search Google Scholar
  • Payne, P.I., 1987. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol. 38:141–153.

    Payne P.I. , 'Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality ' (1987 ) 38 Annu Rev Plant Physiol. : 141 -153.

    • Search Google Scholar
  • Payne, P.I., L.M. Holt, A.F. Krattiger, and J.M. Carillo. 1988. Relationship between seed quality characteristics and HMW glutenin composition determined using wheats grown in Spain. J. Cereal Sci. 7:229–235.

    Carillo J.M. , 'Relationship between seed quality characteristics and HMW glutenin composition determined using wheats grown in Spain ' (1988 ) 7 J. Cereal Sci. : 229 -235.

    • Search Google Scholar
  • Popineau, Y., M. Cornec, J. Lefebvre, and B. Marchylo. 1994. Influence of high Mr glutenin subunits on glutenin polymers and rheological properties of glutens and gluten sub-fractions of near-isogenic lines of wheat Sicco. J. Cereal Sci. 19:231–241.

    Marchylo B. , 'Influence of high Mr glutenin subunits on glutenin polymers and rheological properties of glutens and gluten sub-fractions of near-isogenic lines of wheat Sicco ' (1994 ) 19 J. Cereal Sci. : 231 -241.

    • Search Google Scholar
  • Reddy, P., R. Appels. 1993. Analysis of a genomic DNA segment carrying the high-molecular-weight (HMW) glutenin Bx17 subunit and its use as an RFLP marker. Theor. Appl. Genet. 85: 616–624.

    Appels R. , 'Analysis of a genomic DNA segment carrying the high-molecular-weight (HMW) glutenin Bx17 subunit and its use as an RFLP marker ' (1993 ) 85 Theor. Appl. Genet. : 616 -624.

    • Search Google Scholar
  • Shewry, P.R., N.G. Halford, and A.S. Tatham. 1992. The high molecular weight subunits of wheat glutenin. J. Cereal Sci. 15:105–120.

    Tatham A.S. , 'The high molecular weight subunits of wheat glutenin ' (1992 ) 15 J. Cereal Sci. : 105 -120.

    • Search Google Scholar
  • Shewry, P.R., A.S. Tatham, P. Barro, and P. Lazzeri. 1995. Biotechnology of breadmaking: unraveling and manipulating the multi-protein gluten complex. Biotechnology. 13:1185–1190.

    Lazzeri P. , 'Biotechnology of breadmaking: unraveling and manipulating the multi-protein gluten complex ' (1995 ) 13 Biotechnology : 1185 -1190.

    • Search Google Scholar
  • Shewry, P.R., A.S. Tatham. 1997. Disulphide bonds in wheat gluten proteins. J. Cereal Sci. 25:135–146.

    Tatham A.S. , 'Disulphide bonds in wheat gluten proteins ' (1997 ) 25 J. Cereal Sci. : 135 -146.

    • Search Google Scholar
  • Shewry, P.R., N.G. Halford. 2002. Cereal seed storage proteins: structures, properties and role in grain utilization. J Exp Bot. 53: 947–958.

    Halford N.G. , 'Cereal seed storage proteins: structures, properties and role in grain utilization ' (2002 ) 53 J Exp Bot. : 947 -958.

    • Search Google Scholar
  • Shewry, P.R., S.M. Gilbert, A.W.J. Savage, A.S. Tatham, Y.-F. Wan, P.S. Belton, N. Wellner, R. D’Ovidio, F. Bekes, N.G. Halfond. 2003. Sequence and properties of HMW subunit 1Bx20 from pasta wheat ( Triticum durum ) which is associated with poor end use properties. Theor. Appl. Genet. 106:744–750.

    Halfond N.G. , 'Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum) which is associated with poor end use properties ' (2003 ) 106 Theor. Appl. Genet. : 744 -750.

    • Search Google Scholar
  • Sugiyama, T., A. Rafalski, D. Peterson, D. Soll. 1985. A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res. 13:8729–8737.

    Soll D. , 'A wheat HMW glutenin subunit gene reveals a highly repeated structure ' (1985 ) 13 Nucleic Acids Res. : 8729 -8737.

    • Search Google Scholar
  • Thompson, R.D., D. Bartels, N.P. Harberd. 1985. Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW glutenin subunit. Nucleic Acids Res. 13:6833–6846.

    Harberd N.P. , 'Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW glutenin subunit ' (1985 ) 13 Nucleic Acids Res. : 6833 -6846.

    • Search Google Scholar
  • Wan Y., D. Wang, P.R. Shewry, and N.G. Halford. 2002. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrical . Theor. Appl. Genet. 104:828–839.

    Halford N.G. , 'Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrical ' (2002 ) 104 Theor. Appl. Genet. : 828 -839.

    • Search Google Scholar
  • Wang, J., Z. Yan, Y. Wei, Y. L. Zheng, 2004. A novel high-molecular-weight glutenin subunit gene Ee1.5 from Elytrigia elongata (Host) Nevski. J. Cereal Sci. 40: 289–294.

    Zheng Y. L. , 'A novel high-molecular-weight glutenin subunit gene Ee1.5 from Elytrigia elongata (Host) Nevski ' (2004 ) 40 J. Cereal Sci. : 289 -294.

    • Search Google Scholar
  • Wei, Y.M., Y.L. Zheng, D.C. Liu, Y.H. Zhou, X.J. Lan. 2002. HMW-glutenin and gliadin variations in Tibetan weedrace, Xinjiang rice wheat and Yunnan hulled wheat. Genet Resour Crop Ev. 49:327–330.

    Lan X.J. , 'HMW-glutenin and gliadin variations in Tibetan weedrace, Xinjiang rice wheat and Yunnan hulled wheat ' (2002 ) 49 Genet Resour Crop Ev. : 327 -330.

    • Search Google Scholar
  • Wei, Y.M., Y.L. Zheng, D.C. Liu, Y.H. Zhou, D.C. Liu, X.J. Lan, Z.H. Yan, Z.Q. Zhang. 2001. Genetic diversity of Gli-1, Gli-2 and Glu-1 alleles among Chinese endemic wheats. Acta Bot Sin. 43:834–839

    Zhang Z.Q. , 'Genetic diversity of Gli-1, Gli-2 and Glu-1 alleles among Chinese endemic wheats ' (2001 ) 43 Acta Bot Sin. : 834 -839.

    • Search Google Scholar
  • Yan, Y., J. Zhang, Y. Xiao, J. Yu, Y. Hu, M. Cai, Y. Li, S.L.K. Hsam, F.J. Zeller. 2004. Identification and molecular characterization of a novel y-type Glu-D’1 glutenin gene of Aegilops tauschii . Theor. Appl. Genet. 108:1349–1358.

    Zeller F.J. , 'Identification and molecular characterization of a novel y-type Glu-D’1 glutenin gene of Aegilops tauschii ' (2004 ) 108 Theor. Appl. Genet. : 1349 -1358.

    • Search Google Scholar
  • Yan, Z., Y. Wan, K. Liu, Y.L. Zheng, D.W. Wang. 2002. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Sci Bull. 47:220–225.

    Wang D.W. , 'Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits ' (2002 ) 47 Chinese Sci Bull. : 220 -225.

    • Search Google Scholar
  • Yang, W.Y., C. Yen, J.L. Yang. 1992. Cytogenetic study on the origin of some special Chinese landraces of common wheat. Wheat Infor Serv. 75:14–20.

    Yang J.L. , 'Cytogenetic study on the origin of some special Chinese landraces of common wheat ' (1992 ) 75 Wheat Infor Serv. : 14 -20.

    • Search Google Scholar