Cereal Research Communications
Volume/Issue:
Volume 34: Issue 2-3
Development of inter-varietal chromosome substitution lines of wheat ( Triticum aestivum L.) bearing a rye genetic marker and their verification with microsatellite markers
Authors: T. Efremova 1 , I. Leonova 1 , V. Arbuzova 1 , and L. Laikova 1
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  • 1 Russian Academy of Sciences Institute of Cytology and Genetics, Siberian Branch pr. Lavrent’eva 10 Novosibirsk 630090 Russia
DOI:
https://doi.org/10.1556/crc.34.2006.2-3.227
Page Count:
973–980
Publication Date:
01 Jun 2006
Online Publication Date:
30 Mar 2007
Article Category:
Research Article
Restricted access

Two monosomic alien substitution lines (MAS lines, 2n=41=40+5R) of wheat Triticum aestivum L. cv. ‘Saratovskaya 29’ were used as recipients in the development of inter-varietal substitution lines for chromosomes 5A and 5D. In the MAS lines, chromosomes 5A or 5D of ‘Saratovskaya 29’ were replaced by homoeologous univalent chromosome 5R of rye Secale cereale L. cv. ‘Onokhoiskaya’, which bears the Hp marker gene coding for hairy peduncle. The donors included 18 spring and winter wheat varieties. The MAS lines were developed by crossing monosomic lines of ‘Saratovskaya 29’ for chromosomes 5A and 5D to a wheat-rye substitution line of ‘Saratovskaya 29’ 5R(5D) followed by cytological and morphological selection of plants with chromosome configuration 20II +5RI in metaphase I of pollen mother cells from F 1 and F 2 plants with slightly hairy peduncles. It was shown that MAS lines could be maintained during long-term propagation (18 generations). Use of MAS lines with the Hp marker gene allows acceleration and abbreviation of cytological analysis and elimination of the probability of ‘univalent switch’ in the course of the development of substitution lines. The method was applied to the development of 22 ‘Saratovskaya 29’ lines with inter-varietal substitution for chromosomes 5A and 5D. Fourteen and thirteen microsatellite markers located in chromosomes 5A and 5D, respectively, were used to prove the authenticity of the inter-varietal substitution lines. According to these markers, 21 substitution lines from 22 studied were correct.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
Triticum aestivum L.; Secale cereale L.; monosomic alien substitution lines; chromosome substitutions; microsatellite markers; genetic markers; Hp gene

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