View More View Less
  • 1 Quaid-i-Azam University Department of Biochemistry Islamabad Pakistan
  • 2 University of Arid Agriculture Department of Botany Rawalpindi Pakistan
Restricted access

One hundred and sixty Pakistani Hordeum vulgare ssp. vulgare accessions were analyzed for genetic diversity on the basis of hordein, seed storage proteins. In total we have analyzed 7 Hor-1, 12 Hor-2 and 5 Hor-3 alleles for three hordein loci in barley accessions on SDS-PAGE. Out of 24 polymorphic alleles, three rare alleles (Hor 3.1, Hor 2.1, Hor 1.1) were detected. Abundant genetic variability was observed in Pakistani barley accessions for hordein loci. Genetic similarities calculated for all pair wise comparisons of H. vulgare accessions were used to form 83 banding patterns that represent the core collections of Pakistan, which included 50 unique patterns. Multivariate analysis conducted to generate similarity matrix using Jaccard’s coefficient (Jaccard, 1908) to estimate relatedness and divergence among 160 accessions ranged from 0.11 to 1.00, representing high level of genetic variability. Clustering was carried out to determine genetic diversity among the core collections that clustered them into three major clusters. In this study genetic diversity for cultivated barley belonging to different regions of Pakistan were in the order of Punjab> Balochistan> Northern Area> Sindh> A.J.K.> N.W.F.P.

  • Atanassov, P., Borries, C., Zaharieva, M., Monneveux, P. 2001. Hordein polymorphism and variation of agromorphological traits in a collection of naked barley. Genet. Resource. Crop Evol. 48: 353–360.

    Monneveux P. , 'Hordein polymorphism and variation of agromorphological traits in a collection of naked barley ' (2001 ) 48 Genet. Resource. Crop Evol. : 353 -360.

    • Search Google Scholar
  • Bernardo, A., Luque, A., Cuadrado, A., Negro, A., Jouve, N., Soler, C. 1997. The assessment of genetic variation in Spanish primitive cultivars of barley by a combination of isozyme and hordeins. Genet. Resource. Crop Evol. 44(3): 217–226.

    Soler C. , 'The assessment of genetic variation in Spanish primitive cultivars of barley by a combination of isozyme and hordeins ' (1997 ) 44 Genet. Resource. Crop Evol. : 217 -226.

    • Search Google Scholar
  • Bergal, P., Friedberg, L. 1940. Essai d’identification des orges cultivées en France. Annals Epiphyt. Phytogenetique 6: 2–4.

    Friedberg L. , 'Essai d’identification des orges cultivées en France ' (1940 ) 6 Annals Epiphyt. Phytogenetique : 2 -4.

    • Search Google Scholar
  • Bothmer R. von. 1992. The wild species of Hordeum , relationships and potential use for improvement of cultivated barley. In: Barley: Genetics, Biochemistry, molecular biology and biotechnology, eds., P. R. Shewry, CAB International, Oxford, pp: 3–18.

    Bothmer R. , '', in Barley: Genetics, Biochemistry, molecular biology and biotechnology , (1992 ) -.

  • Cattivelli, L., Komjanc, M., Terzi, V., Odoardi, M. 1987. Identificazione delle varietà di orzo mediante elettroforesi delle ordeine. Sementi Elette 3: 3–8.

    Odoardi M. , 'Identificazione delle varietà di orzo mediante elettroforesi delle ordeine ' (1987 ) 3 Sementi Elette : 3 -8.

    • Search Google Scholar
  • Choumane, W., Achtar, S., Valkoun J., Weigand, F. 1998. Genetic variation in core and base barley collections from WANA as revealed by RAPDs. In: Triticaceae III, ed., A. A. Jaradat, Science Publishers Inc., New Hampshire, USA, pp: 159–163.

    Weigand F. , '', in Triticaceae III , (1998 ) -.

  • Doll, H., Andersen, B. 1981. Preparation of barley storage protein, hordein, for analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 115: 61–66.

    Andersen B. , 'Preparation of barley storage protein, hordein, for analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis ' (1981 ) 115 Anal. Biochem. : 61 -66.

    • Search Google Scholar
  • Echart-Almeida, C., Cavalli-Molina, S. 2000. Hordein polypeptide patterns in relation to malting quality in Brazilian barley varieties. Genet. Mol. Biol. 23(2): 425–433.

    Cavalli-Molina S. , 'Hordein polypeptide patterns in relation to malting quality in Brazilian barley varieties ' (2000 ) 23 Genet. Mol. Biol. : 425 -433.

    • Search Google Scholar
  • Erskine, W., Muehlbauer, F.J. 1991. Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm. Theor. Appl. Genet. 83: 119–125.

    Muehlbauer F.J. , 'Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm ' (1991 ) 83 Theor. Appl. Genet. : 119 -125.

    • Search Google Scholar
  • Frankel, O. H., Brown, A.H.D. 1984. Plant genetic resources today: a critical appraisal. In: Crop genetic resources: conservation and evaluation, eds., J.H.W. Holden, J.T. Williams, G. Allen and U. Winchester, Massachusetts, pp. 249–268.

  • Hintum, T.J.L. 1994. Comparison of marker systems and construction of a core collection in a pedigree of European spring barley. Theor. Appl. Genet. 89: 991–997.

    Hintum T.J.L. , 'Comparison of marker systems and construction of a core collection in a pedigree of European spring barley ' (1994 ) 89 Theor. Appl. Genet. : 991 -997.

    • Search Google Scholar
  • Hoffman, D. L. Bregitzer, P. 1996. Identification of Reproducible PCR-RAPD Markers that Enable the Differentiation of Closely Related Six-Rowed Malting Barley ( Hordeum vulgare L.) Cultivars. J. Am. Soc. Brew. Chem. 54(3): 172–176.

    Bregitzer P. , 'Identification of Reproducible PCR-RAPD Markers that Enable the Differentiation of Closely Related Six-Rowed Malting Barley (Hordeum vulgare L.) Cultivars ' (1996 ) 54 J. Am. Soc. Brew. Chem. : 172 -176.

    • Search Google Scholar
  • Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bull. Soc. Vaudoise Sci. Nat. 44: 223–270.

    Jaccard P. , 'Nouvelles recherches sur la distribution florale ' (1908 ) 44 Bull. Soc. Vaudoise Sci. Nat. : 223 -270.

    • Search Google Scholar
  • Laemmli, U. K. 1970. Cleavage of structural proteins assembly of the head of bacteriophage T4. Nature 22: 680–685.

    Laemmli U. K. , 'Cleavage of structural proteins assembly of the head of bacteriophage T4 ' (1970 ) 22 Nature : 680 -685.

    • Search Google Scholar
  • Mackay, M.C. 1986. Utilizing wheat genetic resources in Australia. In: Proceedings of 5th Assembly Wheat Breed Soc., Australia, ed., R. McLean, Perth/Merredin, Australia, pp: 56–61.

  • Marchylo, B.A., Laberge, D.E. 1981. Barley cultivar identification by electrophoretic analysis of hordein proteins. II. Catalogue of electrophoregram formulae for Canadian-grown barley cultivars. Can. J. Plant Sci. 60: 859–870.

    Laberge D.E. , 'Barley cultivar identification by electrophoretic analysis of hordein proteins. II. Catalogue of electrophoregram formulae for Canadian-grown barley cultivars ' (1981 ) 60 Can. J. Plant Sci. : 859 -870.

    • Search Google Scholar
  • Marchylo, B.A. 1987. Barley cultivar identification by SDS gradient PAGE analysis of hordein. Can. J. Plant Sci. 67: 927–944.

    Marchylo B.A. , 'Barley cultivar identification by SDS gradient PAGE analysis of hordein ' (1987 ) 67 Can. J. Plant Sci. : 927 -944.

    • Search Google Scholar
  • Nielsen, G., Johansen, H.B. 1986. Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isozyme loci of 47 reference varieties. Euphyt. 35: 717–728.

    Johansen H.B. , 'Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isozyme loci of 47 reference varieties ' (1986 ) 35 Euphyt. : 717 -728.

    • Search Google Scholar
  • Radovic, D., Vapa, L. 1996. Hordein composition of Yugoslav barley cultivars. Cereal Res. Comm. 24(3): 331–337.

    Vapa L. , 'Hordein composition of Yugoslav barley cultivars ' (1996 ) 24 Cereal Res. Comm. : 331 -337.

    • Search Google Scholar
  • Saghai-Maroof, M.A., Biyashev, R.M., Yang, G.P., Zhang, Q., Allard, R.W. 1994. Extraordinarily polymorphic microsatellite DNA in barley: Species diversity, chromosomal locations, and population dynamics. Proc. Nat. Acad. Sci. 91: 5466–5470.

    Allard R.W. , 'Extraordinarily polymorphic microsatellite DNA in barley: Species diversity, chromosomal locations, and population dynamics ' (1994 ) 91 Proc. Nat. Acad. Sci. : 5466 -5470.

    • Search Google Scholar
  • Shewry, P.R., Ellis, J.R.S., Pratt, H.M., Miflin, B.J. 1978a. A comparison of methods for the extraction and separation of hordein fractions from 29 barley varieties. J. Sci. Food Agric. 29: 433–441.

    Miflin B.J. , 'A comparison of methods for the extraction and separation of hordein fractions from 29 barley varieties ' (1978 ) 29 J. Sci. Food Agric. : 433 -441.

    • Search Google Scholar
  • Shewry, P.R., Faulks, A.J., Pickering, R.A., Jones, I.T., Finch R.A., Miflin, B.J. 1980. The genetic analysis of barley storage proteins. Hered. 44: 383–389.

    Miflin B.J. , 'The genetic analysis of barley storage proteins ' (1980 ) 44 Hered. : 383 -389.

    • Search Google Scholar
  • Thompson, J.A., Nelson R.L., Vodkin, L.O. 1998. Identification of diverse soybean germplasm using RAPD markers. Crop Sci. 38: 1348–1355.

    Vodkin L.O. , 'Identification of diverse soybean germplasm using RAPD markers ' (1998 ) 38 Crop Sci. : 1348 -1355.

    • Search Google Scholar
  • Vapa, L., Radovic, D. 1998. Genetics and molecular biology of barley hordeins. Cereal Res. Comm. 26(1): 31–38.

    Radovic D. , 'Genetics and molecular biology of barley hordeins ' (1998 ) 26 Cereal Res. Comm. : 31 -38.

    • Search Google Scholar
  • Virk, P.S., Newbury, H.J., Jackson, M.T., Ford-Lloyd, B.V. 1995. The identification of duplicate accessions within a rice germplasm collection using RAPD analysis. Theor. Appl. Genet. 90: 1049–1055.

    Ford-Lloyd B.V. , 'The identification of duplicate accessions within a rice germplasm collection using RAPD analysis ' (1995 ) 90 Theor. Appl. Genet. : 1049 -1055.

    • Search Google Scholar
  • Yin, O. Y., Ma, D.O., Ding, Y. 2003. Analysis of Genetic diversity of hordein in wild close relatives of barley from Tibet. Theor. Appl. Genet. 107: 837–842.

    Ding Y. , 'Analysis of Genetic diversity of hordein in wild close relatives of barley from Tibet ' (2003 ) 107 Theor. Appl. Genet. : 837 -842.

    • Search Google Scholar
  • Zhang, Q., Sahgai-Maroof, M. A., Kleinhofs, A. 1993. Comparative diversity analysis of RFLPs and isozymes within and among populations of Hordeum vulgare ssp. spontaneum . Genetics 134: 901–911.

    Kleinhofs A. , 'Comparative diversity analysis of RFLPs and isozymes within and among populations of Hordeum vulgare ssp. spontaneum ' (1993 ) 134 Genetics : 901 -911.

    • Search Google Scholar