Cereal Research Communications
Volume/Issue:
Volume 35: Issue 1
Identification of novel variants of High Molecular Weight Glutenin Subunits in Indian bread wheat landraces
Authors:
A. Singh Indian Agricultural Research Institute Division of Genetics New Delhi 110012 India

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,
J. Deveshwar Indian Agricultural Research Institute Division of Genetics New Delhi 110012 India

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A. Ahlawat Indian Agricultural Research Institute Division of Genetics New Delhi 110012 India

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, and
B. Singh Indian Agricultural Research Institute Division of Genetics New Delhi 110012 India

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Pages:
99–108
Online Publication Date:
23 Mar 2007
Publication Date:
01 Mar 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.35.2007.1.12
Keywords:
High Molecular Weight Glutenin Subunits; wheat; novel subunits; Glu-1
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The High Molecular Weight Glutenin Subunits (HMW GS) in bread wheat landraces were studied using the SDS-PAGE technique. Out of the 32 landraces, 23 were homogeneous while nine showed heterogeneity with respect to the HMW glutenin subunits. Novel variants were observed in HMW GS in four of the landraces. One novel subunit coded by the Glu-B1x locus and two novel subunit pairs at the Glu-D1 locus were identified. A modified system of nomenclature over that of Payne and Lawrence (1983) is suggested for numbering the new subunits. Accordingly, the novel subunits are numbered as 7 1 ( Glu-B1x ) and 2+12 2 ; 5 1 +12 2 ; 5 2 +12 3 and 5 3 +12 3 ( Glu-D1x + y ) and the allelic designations are given as Glu-B1 bh for 7 1 +9; Glu-D1bp for 2+12 2 . Glu-D1bq for 5 1 +12; Glu-D1br for 5 1 +12 2 ; GluD1br for 5 2 +12 3 and GluD1bt for 5 3 +12 3 .

  • Bietz, J.A., Shepherd, K.W., Wall, J.S. 1975. Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem. 52 :513–532.

    Wall J.S. , 'Single-kernel analysis of glutenin: use in wheat genetics and breeding ' (1975 ) 52 Cereal Chem. : 513 -532 .

    • Search Google Scholar

  • Branlard, G., Dardevet, M. 1985. Diversity of grain proteins and bread wheat quality. I. Correlation between gliadin bands and flour quality characteristics. J. Cereal Sci. 3 :329–343.

    Dardevet M. , 'Diversity of grain proteins and bread wheat quality. I. Correlation between gliadin bands and flour quality characteristics ' (1985 ) 3 J. Cereal Sci. : 329 -343 .

    • Search Google Scholar

  • Gupta, R.K. 2004. HMW glutenin subunits in: T aestivum . In: Gupta, R.K. et al. (eds). DWR Progress Report, 2004, Wheat Quality, AICW&BIP Section, p. 41.

  • http://shigen.lab.nig.ac.jp/wheat/komugi/genes/download.jsp for HMWGS designations and gene symbols

  • Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteria phage T4. Nature 227 :680–685.

    Laemmli U.K. , 'Cleavage of structural proteins during the assembly of the head of bacteria phage T4 ' (1970 ) 227 Nature : 680 -685 .

    • Search Google Scholar

  • Lafiandra, D., Ovidio, R., Porcedu, E., Marigiotta, B. 1993. New data supporting High Mr Glutenin Subunit 5 as determinant of quality differences among the pairs 5+10 and 2+12. J. Cereal Science 18 :197–205.

    Marigiotta B. , 'New data supporting High Mr Glutenin Subunit 5 as determinant of quality differences among the pairs 5+10 and 2+12 ' (1993 ) 18 J. Cereal Science : 197 -205 .

    • Search Google Scholar

  • Lagudah, E.S., Halloran, G.M. 1988. Phytogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat. I Variation in HMW subunits of glutenins and gliadins. Theor. Appl. Genet. 75 :592–598.

    Halloran G.M. , 'Phytogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat. I Variation in HMW subunits of glutenins and gliadins ' (1988 ) 75 Theor. Appl. Genet. : 592 -598 .

    • Search Google Scholar

  • Levy, A.A., Feldman, M. 1988. Ecologeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat Triticum turgidum var. dicoccoides . Theor. Appl. Genet: 75 :651–658.

    Feldman M. , 'Ecologeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat Triticum turgidum var. dicoccoides ' (1988 ) 75 Theor. Appl. Genet : 651 -658 .

    • Search Google Scholar

  • Marigiotta, B., Colaprico, G., D’Ovidio, R., Laflianda, D. 1993. Characterization of high-Mr Subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoresis separations and restriction fragment length polymorphism (RFLP) analyses of their encoding genes. J. Cereal Sci. 17 :221–236.

    Laflianda D. , 'Characterization of high-Mr Subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoresis separations and restriction fragment length polymorphism (RFLP) analyses of their encoding genes ' (1993 ) 17 J. Cereal Sci. : 221 -236 .

    • Search Google Scholar

  • Misra, B.K., Singh, Anju M., Deveshwar, J.J., Pandey, H.N., Ahlawat, A.K. 2005. ’Pissi Local’ an Indian Wheat Germplasm with Unique Quality Features. Abstracts: Poster presented at National Symposium on “Wheat Improvement for the Tropical Areas” held at Coimbatore, September 23–25 P. Full paper in press.

  • Ng, P.K.W., Bushuk, W. 1988. Statistical relationships between high molecular weight subunits of glutenin and breadmaking quality of Canadian grown wheats. Cereal Chem. 65 (5):408–413.

    Bushuk W. , 'Statistical relationships between high molecular weight subunits of glutenin and breadmaking quality of Canadian grown wheats ' (1988 ) 65 Cereal Chem. : 408 -413 .

    • Search Google Scholar

  • Ng, P.K.W., Bushuk, W. 1989. Concerning the nomenclature of high molecular weight glutenin subunits. J. Cereal Science 11 :53–60.

    Bushuk W. , 'Concerning the nomenclature of high molecular weight glutenin subunits ' (1989 ) 11 J. Cereal Science : 53 -60 .

    • Search Google Scholar

  • Payne, P.I., Lawrence, C.J. 1983. Catalogue of alleles for the complex gene loci, Glu A-1, Glu B-1 and Glu D-1 , which code for the high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11 :29–35.

    Lawrence C.J. , 'Catalogue of alleles for the complex gene loci, Glu A-1, Glu B-1 and Glu D-1, which code for the high molecular weight subunits of glutenin in hexaploid wheat ' (1983 ) 11 Cereal Res. Comm. : 29 -35 .

    • Search Google Scholar

  • Payne, P.I., Law, C.N., Mudd, E.E. 1980. Control by homeologous group/chromosomes of high molecular weight subunits of glutenin, a major storage protein of wheat endosperm. Theor. Appl. Genet. 58 :113–120.

    Mudd E.E. , 'Control by homeologous group/chromosomes of high molecular weight subunits of glutenin, a major storage protein of wheat endosperm ' (1980 ) 58 Theor. Appl. Genet. : 113 -120 .

    • Search Google Scholar

  • Payne, P.I., Nightingale, M.A., Kratliger, A.F., Holt, L.M. 1987. The relationship between HMW glutenin subunit composition and the bread making quality of British grown wheat varieties. J. Sci. Good Agric. 40 :51–56.

    Holt L.M. , 'The relationship between HMW glutenin subunit composition and the bread making quality of British grown wheat varieties ' (1987 ) 40 J. Sci. Good Agric. : 51 -56 .

    • Search Google Scholar

  • Pogna, N.E., Mellini, F., Beretta, A., Dalbelin Peruffo, A. 1989. The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy. J. Genet and Breed. 43 :17–24.

    Dalbelin Peruffo A. , 'The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy ' (1989 ) 43 J. Genet and Breed. : 17 -24 .

    • Search Google Scholar

  • Shewry, P.R., Popinean, Y., Lafiandra, D., Belton, P. 2001. Wheat glutenin subunits and dough elasticity. Trends Food Sci. Technol. 11 :433–441.

    Belton P. , 'Wheat glutenin subunits and dough elasticity ' (2001 ) 11 Trends Food Sci. Technol. : 433 -441 .

    • Search Google Scholar

  • Shewry, P.R., Napier, J.A., Tatham, A.S. 1995. Seed storage proteins: Structures and biosynthesis. The Plant Cell 7 :845–956.

    Tatham A.S. , 'Seed storage proteins: Structures and biosynthesis ' (1995 ) 7 The Plant Cell : 845 -956 .

    • Search Google Scholar

  • Sreeramulu, G., Singh, N.K. 1994. High molecular weight glutenin subunits of Indian wheat cultivars: Association of subunits 1BL/1RS wheat-rye translocation. J. Cereal Sci. 20 :217–225.

    Singh N.K. , 'High molecular weight glutenin subunits of Indian wheat cultivars: Association of subunits 1BL/1RS wheat-rye translocation ' (1994 ) 20 J. Cereal Sci. : 217 -225 .

    • Search Google Scholar

  • Tahir, M., Pavoni, A., Tucci, G.F., Turchetta, T., Lafiandra, D. 1996. Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat. Theor. Appl. Genet. 92 :654–659.

    Lafiandra D. , 'Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat ' (1996 ) 92 Theor. Appl. Genet. : 654 -659 .

    • Search Google Scholar

  • Waines, J.G., Payne, P.I. 1987. Electrophoretic analysis of the high molecular weight glutenin subunits of Triticum monococcum. T. urartu and the A genome of bread wheat ( Triticum aestivum ). Theor. Appl. Genet. 74 :71–76.

    Payne P.I. , 'Electrophoretic analysis of the high molecular weight glutenin subunits of Triticum monococcum. T. urartu and the A genome of bread wheat (Triticum aestivum) ' (1987 ) 74 Theor. Appl. Genet. : 71 -76 .

    • Search Google Scholar

  • Wan, Y., Wang, D., Shewry, P.R., Halford, N.G. 2002. Isolation and characterization of five novel high molecular weight subunits of glutenin genes from Triticum timopheevi and Aegilops cylindrical . Theor. Appl. Genet. 104 :828–839.

    Halford N.G. , 'Isolation and characterization of five novel high molecular weight subunits of glutenin genes from Triticum timopheevi and Aegilops cylindrical ' (2002 ) 104 Theor. Appl. Genet. : 828 -839 .

    • Search Google Scholar

  • William, M.D.H.M., Pena, R.J., Mujeeb-Kazi, A. 1993. Seed protein and isozyme variations in Triticum tauschii (Aegilops squarrosa) . Theor. Appl. Genet. 87 :257–263.

    Mujeeb-Kazi A. , 'Seed protein and isozyme variations in Triticum tauschii (Aegilops squarrosa) ' (1993 ) 87 Theor. Appl. Genet. : 257 -263 .

    • Search Google Scholar
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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
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Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
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Springer Nature Switzerland AG
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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