Cereal Research Communications
Volume/Issue:
Volume 35: Issue 3
Characterization of left-border flanking sequences of T-DNA integration in transgenic rice ( Oryza sativa L.) expressing cry1Ab
Authors:
M. Zaidi University of Ottawa Agricultural Biotechnology Laboratories, Department of Biochemistry, Microbiology and Immunology 451 Smyth Rd. Ottawa ON K1H 8M5 Canada

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,
X. Cheng

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, and
I. Altosaar

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Pages:
1375–1383
Online Publication Date:
07 Aug 2007
Publication Date:
01 Sep 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.35.2007.3.2
Keywords:
rice; cry1Ab ; Agrobacterium ; T-DNA; left-border; right-border; repeat DNA sequence; promoter
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To understand the molecular details of T-DNA integration, the left border (LB) sequences and flanking plant DNA of 16 independent T-DNA insertions in transgenic cry1Ab rice were analyzed by an inverse PCR approach. DNA sequencing indicated that five of the 16 fragments (31%) were found to have simple or rearranged tandem repeats of right border sequences in a head to tail fashion. Mirror truncations of LB of the T-DNA, as well as mirror rearrangements, such as point mutations, small deletions and inversions were found in the region close to the LB breakpoints in some inserts. Host plant DNA flanking the T-DNA endpoints were also sequenced. The A+T contents in the plant DNA within 50 bp adjacent to the T-DNA endpoints were between 30–76% (average 52.5%), not different from the average genome value. Despite minor mutations and some rearrangements, it appears that T-DNA, harbouring a synthetic cry1Ab coding sequence of 49% GC (as well as uidA and hph ), still carries such a foreign gene into ‘transcriptionally active regions’ of the rice genome, which are 55.8% GC on average as predicted from the rice genome sequence.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

 

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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