Cereal Research Communications
Volume/Issue:
Volume 35: Issue 3
Evaluation of wheat germplasm from the Madeira and Canary archipelagos using a single molecular marker. A rapid screening method for identification of durum wheat accessions
Authors: V. Andrade 1 , T. Santos 1 , D. Afonso Morales 2 , G. Costa 1 and M. Pinheiro de Carvalho 1
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  • 1 University of Madeira ISOPlexis/BIOtecMOL, Centre of Macaronesian Studies Campus da Penteada 9000-390 Funchal Portugal
  • 2 Centro de Conservación de la Biodiversidad Agrícola de Tenerife Tacaronte, Tenerife Spain
DOI:
https://doi.org/10.1556/crc.35.2007.3.4
Page Count:
1397–1404
Publication Date:
01 Sep 2007
Online Publication Date:
07 Aug 2007
Article Category:
Research Article
Restricted access

This work aims to present a rapid and precise screening method of wheat germplasm and identification of durum wheat accessions in germplasm collections. Fifty-two wheat accessions maintained in the ISOPlexis germplasm bank at the Madeira University, Portugal, and 72 accessions from the Centre for Conservation of Crop Biodiversity of Tenerife (CCBAT), Spain, have been screened for their specific ploidy status using the molecular marker Dgas44. We have demonstrated that the Dgas44 sequence is effective in the screening of Madeiran and Canarian wheat accessions. This screening method permitted the detection of 10 and 11 durum accessions among Madeiran and Canarian wheats, accounting for 19.2 and 15.3% of screened collections, respectively. The obtained results have shown a 100% of cases correspondence with the previously performed morphological identification of the Madeiran wheats. It also permitted rectification and clarification of previous classification of some accessions based only on the morphological traits. The PCR based assay was directly applicable to the screening of seeds and was suitable for detecting seed mixtures in accessions. This rapid method has been proven to be a useful tool in gene bank accessions management including verification of their ploidy status and detection of seed lots adulteration.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
durum wheat; germplasm; D genome; DNA; PCR; Dgas44

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