Cereal Research Communications
Volume/Issue:
Volume 35: Issue 3
Changes in total- and α -amylase activities and wheat germ agglutinin content in wide-range herbicide resistant wheat lines
Authors: Á. Halász, E. Horváth-Szanics 1 , M. Nagy-Gasztonyi 1 , J. Pauk 2 and Gy. Hajós 1
View More View Less
  • 1 Central Food Research Institute Department of Nutrition Science Herman O. út 15 H-1022 Budapest Hungary
  • 2 Cereal Research Non-Profit Company P.O. Box 391 H-6701 Szeged Hungary
DOI:
https://doi.org/10.1556/crc.35.2007.3.5
Page Count:
1405–1413
Publication Date:
01 Sep 2007
Online Publication Date:
07 Aug 2007
Article Category:
Research Article
Restricted access

Protein sets, enzyme activities and immune reactivity against wheat germ agglutinin in the albumin-globulin fractions of parent and herbicide resistant transgenic wheat lines were studied.Our results showed significantly increased amylase activities and increased immune reactivity against wheat germ agglutinin in the herbicide resistant transgenic wheat lines, investigated. The amylases and lectins belong to the plant food allergens; this explains why both scientists and consumers are interested in assessing the allergenic potential of plant proteins and the safety assessment of novel foods and GM foods in highlight of food safety. This paper is an important contribution to our database and the understanding of what is going on with genetic engineering of crop plants.

  • Aalberse, R.C. 2000. Structural biology of allergens. Journal of Allergy and Clinical Immunology 106 :228–238.

    Aalberse R.C. , 'Structural biology of allergens ' (2000 ) 106 Journal of Allergy and Clinical Immunology : 228 -238.

    • Search Google Scholar

  • Adessi, C., Miege, C., Albrieux, C., Rabilloud, T. 1997. Two-dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients. Electrophoresis 18 :127–135.

    Rabilloud T. , 'Two-dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients ' (1997 ) 18 Electrophoresis : 127 -135.

    • Search Google Scholar

  • Alrefai, R. 2002. Biotechnology Consultation Note to the File BNF No. 000063, U. S., Food and Drug Administration, Center for Food Safety and Applied Nutrition.

  • Barro, F., Barcelo, P., Lazzeri, P.A., Shewry, P.R., Martin, A., Ballesteros, J. 2002. Field evaluation and agronomic performance of transgenic wheat. Theoretical and Applied Genetics 105 :980–984.

    Ballesteros J. , 'Field evaluation and agronomic performance of transgenic wheat ' (2002 ) 105 Theoretical and Applied Genetics : 980 -984.

    • Search Google Scholar

  • Barro, F., Barcelo, P., Lazzeri, P.A., Shewry, P.R., Martin, A., Ballesteros, J. 2003. Functional properties and agronomic performance of transgenic Tritordeum expressing high molecular weight glutenin subunit genes 1Ax1 and 1Dx5. Journal of Cereal Science 37 :65–70.

    Ballesteros J. , 'Functional properties and agronomic performance of transgenic Tritordeum expressing high molecular weight glutenin subunit genes 1Ax1 and 1Dx5 ' (2003 ) 37 Journal of Cereal Science : 65 -70.

    • Search Google Scholar

  • Becker, D., Brettschneider, R., Lörz, H. 1994. Fertile transgenic wheat plants from microprojectile bombardment of scutellar tissue. Plant Journal 5 :299–307.

    Lörz H. , 'Fertile transgenic wheat plants from microprojectile bombardment of scutellar tissue ' (1994 ) 5 Plant Journal : 299 -307.

    • Search Google Scholar

  • Christensen, A.H., Quail, P.H. 1996. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monootyledonous plants. Transgenic Research 5 :213–218.

    Quail P.H. , 'Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monootyledonous plants ' (1996 ) 5 Transgenic Research : 213 -218.

    • Search Google Scholar

  • Decker, L.A. 1977. Worthington Enzyme Manual. Worthington Biochemical Corp., Freehold, NJ. pp. 173–176.

    Decker L.A. , '', in Worthington Enzyme Manual , (1977 ) -.

  • Felföldi, K., Purnhuaser, L. 1992.: Induction of regenerating callus from immature embryos of 44 wheat and 3 triticale cultivars. Cereal Res. Comm. 20 :273–277.

    Purnhuaser L. , 'Induction of regenerating callus from immature embryos of 44 wheat and 3 triticale cultivars ' (1992 ) 20 Cereal Res. Comm. : 273 -277.

    • Search Google Scholar

  • Hajós, Gy., Gelencsér, É., Grant, G., Bardocz, S., Sakhri, M., Duguid, T.J., Newman, A.M., Pusztai, A. 1996. Effect of proteolytic modification and methionine enrichment on the nutritional value of soya albumins for rats. J. Nutritional Biochemistry 7 :481–487.

    Pusztai A. , 'Effect of proteolytic modification and methionine enrichment on the nutritional value of soya albumins for rats ' (1996 ) 7 J. Nutritional Biochemistry : 481 -487.

    • Search Google Scholar

  • Horváth-Szanics, E., Szabó, Z., Janáky, T., Pauk, J., Hajós, Gy. 2006. Proteomics as an emergent tool for identification of stress-induced proteins in control and genetically modified wheat lines. Chromatographia 63 :S143–S147.

    Hajós Gy. , 'Proteomics as an emergent tool for identification of stress-induced proteins in control and genetically modified wheat lines ' (2006 ) 63 Chromatographia : S143 -S147.

    • Search Google Scholar

  • Krisch, J., Horváth, E., Vágvölgyi, Cs., Tanács, L. 2007. Mobility of herbicides and fungicides in soil and their effects on soil microorganism. Cereal Res. Comm. 35 :673–676.

    Tanács L. , 'Mobility of herbicides and fungicides in soil and their effects on soil microorganism ' (2007 ) 35 Cereal Res. Comm. : 673 -676.

    • Search Google Scholar

  • Matucci, A., Veneri, G., Dalla Pellegrina, C., Zoccatelli, G., Vincenzi, S., Chignola, R., Peruffo, A.D.B., Rizzi, C. 2004. Temperature-dependent decay of wheat germ agglutinin activity and its implications for food processing and analysis. Food Control 15 :391–395.

    Rizzi C. , 'Temperature-dependent decay of wheat germ agglutinin activity and its implications for food processing and analysis ' (2004 ) 15 Food Control : 391 -395.

    • Search Google Scholar

  • Matuz, J., Póka, R., Boldizsár, I., Szerdahelyi, E., Hajós, Gy. 2000. Structure and potential allergenic character of cereal proteins. II. Potential allergens in cereal samples. Cereal Res. Comm. 28 :433–442.

    Hajós Gy. , 'Structure and potential allergenic character of cereal proteins. II. Potential allergens in cereal samples ' (2000 ) 28 Cereal Res. Comm. : 433 -442.

    • Search Google Scholar

  • Mills, E.N.C., Madsen, C., Shewry, P.R., Wichers, H.J. 2003. Bubble formation and stabilization in bread dough. Trends in Food Science & Technology 14 :145–156.

    Wichers H.J. , 'Bubble formation and stabilization in bread dough ' (2003 ) 14 Trends in Food Science & Technology : 145 -156.

    • Search Google Scholar

  • Nehra, N.S., Chibbar, R.N., Leung, N., Caswell, K., Mallard, C., Steinhauser, L., Baga, M., Kartha, K.K. 1994. Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant Journal 5 :285–297.

    Kartha K.K. , 'Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs ' (1994 ) 5 Plant Journal : 285 -297.

    • Search Google Scholar

  • Osborne, T.B. 1907. The Proteins of the Wheat Kernel. Carnegie Institute, Washington.

    Osborne T.B. , '', in The Proteins of the Wheat Kernel , (1907 ) -.

  • O’Farrell, P.H. 1975. High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250 :4007–4021.

    O’Farrell P.H. , 'High resolution two-dimensional electrophoresis of proteins ' (1975 ) 250 Journal of Biological Chemistry : 4007 -4021.

    • Search Google Scholar

  • Pauk, J., Hänsch, R., Schwarz, G., Nerlich, A., Monostori, T., Mészáros, A., Jenes, B., Kertész, Z., Matuz, J., Schulze, J., Mendel, R.R. 1998. Transzgénikus búza ( Triticum aestivum L.) előállítása Magyarországon [Genetic transformation of wheat ( Triticum aestivum L.) in Hungary]. Növénytermelés 47 :241–251.

    Mendel R.R. , 'Transzgénikus búza (Triticum aestivum L.) előállítása Magyarországon ' (1998 ) 47 Növénytermelés : 241 -251.

    • Search Google Scholar

  • Penaa, L.B., Pasquinia, L.A., Tomaroa, M.L., Callego, S.M. 2006. Proteolytic system in sunflower ( Helianthus annuus L.) leaves under cadmium stress: Plant Science 171 :531–537.

    Callego S.M. , 'Proteolytic system in sunflower (Helianthus annuus L.) leaves under cadmium stress ' (2006 ) 171 Plant Science : 531 -537.

    • Search Google Scholar

  • Przymusiski, R., Ruciska, R., Gwóźdź, E.A. 2004. Increased accumulation of pathogenesis-related proteins in response of lupine roots to various abiotic stresses. Environmental and Experimental Botany 52 :53–61.

    Gwóźdź E.A. , 'Increased accumulation of pathogenesis-related proteins in response of lupine roots to various abiotic stresses ' (2004 ) 52 Environmental and Experimental Botany : 53 -61.

    • Search Google Scholar

  • Pusztai, Á., Ewen, S.W.B., Grant, G., Brown, D.S., Stewart, J.C., Peumans, W.J., Van Damme, E.J.M., Bardocz, Zs. 1993. Antinutritive effects of wheat germ agglutinin and other N -acetylglucosamine-specific lectins. Br. J. Nutr. 70 :313–321.

    Bardocz Zs. , 'Antinutritive effects of wheat germ agglutinin and other N-acetylglucosamine-specific lectins ' (1993 ) 70 Br. J. Nutr. : 313 -321.

    • Search Google Scholar

  • Rakszegi, M., Tamás, C., Szűcs, P., Tamás, L., Bedő, Z. 2001. Current status of wheat transformation. Journal of Plant Biotechnology 3 :67–81.

    Bedő Z. , 'Current status of wheat transformation ' (2001 ) 3 Journal of Plant Biotechnology : 67 -81.

    • Search Google Scholar

  • Rakszegi, M., Békés, F., Láng, L., Tamás, L., Shewry, P.R., Bedő, Z. 2005. Technological quality of transgenic wheat expressing an increased amount of a HMW glutenin subunit. Journal of Cereal Science 42 :15–23.

    Bedő Z. , 'Technological quality of transgenic wheat expressing an increased amount of a HMW glutenin subunit ' (2005 ) 42 Journal of Cereal Science : 15 -23.

    • Search Google Scholar

  • Van Damme, E.J.M., Peumans, W.J., Pusztai, Á., Bardocz, Zs. 1998. Handbook of plant lectins: properties and biomedical applications. John Wiley and Sons, West Sussex.

    Bardocz Zs. , '', in Handbook of plant lectins: properties and biomedical applications , (1998 ) -.

    • Search Google Scholar

  • Van der Maarel, M.J.E.C., Van der Veen, B., Uitdehaag, J.C.M., Leemhuis, H., Dijkhuizen, L. 2002. Properties and applications of starch-converting enzymes of the α -amylase family. Journal of Biotechnology 94 :137–155

    Dijkhuizen L. , 'Properties and applications of starch-converting enzymes of the α-amylase family ' (2002 ) 94 Journal of Biotechnology : 137 -155.

    • Search Google Scholar

  • Vasil, V., Srivastava, V., Castillo, A.M., Fromm M., Vasil, I.K. 1993. Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11 :1553–1558.

    Vasil I.K. , 'Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos ' (1993 ) 11 Bio/Technology : 1553 -1558.

    • Search Google Scholar

  • Weeks, J.T., Anderson, O.D., Blechl, A.E. 1993. Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum) . Plant Physiology 102 :1077–1084.

    Blechl A.E. , 'Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum) ' (1993 ) 102 Plant Physiology : 1077 -1084.

    • Search Google Scholar

  • Weil, J.H. 2005. Are genetically modified plants useful and safe? IUBMB Life 57 :311–314.

    Weil J.H. , 'Are genetically modified plants useful and safe? ' (2005 ) 57 IUBMB Life : 311 -314.

    • Search Google Scholar
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
transgenic wheat lines; WGA; amylase activity; food safety; 2D-electrophoresis; immunoblot

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2020 0 0 0
Jun 2020 0 3 0
Jul 2020 0 0 0
Aug 2020 4 0 0
Sep 2020 0 0 0
Oct 2020 0 0 0
Nov 2020 0 0 0