Cereal Research Communications
Volume/Issue:
Volume 35: Issue 3
The effect of seed size on tissue culture response of callus from endosperm-supported mature embryos of barley ( Hordeum vulgare L.)
Authors:
M. Özgen Ankara University Department of Field Crops, Faculty of Agriculture 06110 Diskapi, Ankara Turkey

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M. Yildiz Ankara University Department of Field Crops, Faculty of Agriculture 06110 Diskapi, Ankara Turkey

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N. Koyuncu Ankara University Department of Field Crops, Faculty of Agriculture 06110 Diskapi, Ankara Turkey

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S. Önde Middle East Technical University Department of Biological Sciences, Faculty of Science and Art 06531 Ankara Turkey

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Pages:
1415–1425
Online Publication Date:
07 Aug 2007
Publication Date:
01 Sep 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.35.2007.3.6
Keywords:
barley; callus induction; germination; plant regeneration; seed size
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Although immature embryos are the most suitable explants to regenerate cereals, their limited availability throughout the year hampers frequent experimentation. In order to circumvent this problem, endosperm-supported mature embryos, which are available throughout the year, are proposed for utilization in cereal tissue culture studies. In the present study, the effect of seed size on in vitro seed germination, seedling growth, callus induction and plant regeneration, as well as the relationships between these parameters, were investigated in three barley ( Hordeum vulgare L.) genotypes. Seeds were designated as large or small for each genotype and seed germinations were achieved in Petri dishes between two sheets of pre-wetted filter paper. Germination percentages of seeds, seedling growth rates, shoot and root lengths, and seedling fresh and dry weights were examined. Mature embryos from imbibed and dehulled seeds were aseptically moved slightly with a scalpel. For callus induction, 8 mg 1 −1 , 2,4-D dissolved in water, and for plant regeneration, hormone-free MS medium, was utilized. Highest values with respect to seed germination percentage, seedling growth, callus induction and plant regeneration were obtained using large seeds. Rooting of regenerated shoots and plantlet recovery were also successfully achieved. Based on the significant positive correlations observed between all parameters, we concluded that cultures derived from endosperm-supported mature embryos within large seeds should be employed for achieving superior tissue culture response.

  • Aiken, G.E., Springer, T.L. 1995. Seed size distribution, germination, and emergence of 6 switchgrass cultivars. Journal of Range Management 48 :455–458.

    Springer T.L. , 'Seed size distribution, germination, and emergence of 6 switchgrass cultivars ' (1995 ) 48 Journal of Range Management : 455 -458 .

    • Search Google Scholar

  • Anonymous. 1985. Seed Science and Technology. ISTA, Vol. 13. No. 2.

  • Bonfil, C. 1998. The effect of seed size, cotyledon reserves, and herbivory on seedling survival and growth in Quercus rugosa and Q. laurina (Fagaceae). American Journal of Botany 85 :79–87.

    Bonfil C. , 'The effect of seed size, cotyledon reserves, and herbivory on seedling survival and growth in Quercus rugosa and Q. laurina (Fagaceae) ' (1998 ) 85 American Journal of Botany : 79 -87 .

    • Search Google Scholar

  • Ching, T.M., Rynd, L. 1978. Developmental differences in embryos of high and low protein wheat seeds during germintion. Plant Physiology 62 :866–870.

    Rynd L. , 'Developmental differences in embryos of high and low protein wheat seeds during germintion ' (1978 ) 62 Plant Physiology : 866 -870 .

    • Search Google Scholar

  • Dale, P.J., Deambrogio, E.A. 1979. Comparison of callus induction and plant regeneration from different explants of Hordeum vulgare . Zeitschrift für Pflanzenphysiologie 94 :65–77.

    Deambrogio E.A. , 'Comparison of callus induction and plant regeneration from different explants of Hordeum vulgare ' (1979 ) 94 Zeitschrift für Pflanzenphysiologie : 65 -77 .

    • Search Google Scholar

  • Goldstein, C.S., Kronstad, W.E. 1986. Tissue culture and plant regeneration from immature embryo explants of barley, Hordeum vulgare L. Theoretical and Applied Genetic 71 :631–636.

    Kronstad W.E. , 'Tissue culture and plant regeneration from immature embryo explants of barley, Hordeum vulgare L ' (1986 ) 71 Theoretical and Applied Genetic : 631 -636 .

    • Search Google Scholar

  • Green, J.C., Bransby, D.I. 1995. Effects of seed size on germination and seedling growth of ‘Alamo’ switchgrass. In: West, N.E. (ed.), Proc. Int. Rangel. Congr., 5 th , Salt Lake City, UT. 23–28 July 1995. Soc. for Range Management, Denver, CO, Vol. 1, pp. 183–184.

  • Gupta, S.K., Pathak, P.S., Roy, R.D. 1983. Seedling growth of Leucaena leucocephala (Lam.) de Wit. II. Effect of seed size. Indian Journal of Forestry 6 :202–204.

    Roy R.D. , 'Seedling growth of Leucaena leucocephala (Lam.) de Wit. II. Effect of seed size ' (1983 ) 6 Indian Journal of Forestry : 202 -204 .

    • Search Google Scholar

  • Hanzel, J.J., Miller, J.P., Brinkmann, M.A., Fendos, E. 1985. Genotype and media effects on callus formation and regeneration in barley. Crop Science 25 :27–31.

    Fendos E. , 'Genotype and media effects on callus formation and regeneration in barley ' (1985 ) 25 Crop Science : 27 -31 .

    • Search Google Scholar

  • Jelaska, S., Rengel, Z., Cesar, V. 1984. Plant regeneration from mesocotyl callus of Hordeum vulgare L. Plant Cell Reports 3 :125–129.

    Cesar V. , 'Plant regeneration from mesocotyl callus of Hordeum vulgare L ' (1984 ) 3 Plant Cell Reports : 125 -129 .

    • Search Google Scholar

  • Kato, K., Chowdhury, S.H., Harada, S. 1991. Effect of culture condition on plant regeneration capacity of mature embryo derived callus in wheat ( Triticum aestivum L.). Wheat Information Service 72 :95–97.

    Harada S. , 'Effect of culture condition on plant regeneration capacity of mature embryo derived callus in wheat (Triticum aestivum L.) ' (1991 ) 72 Wheat Information Service : 95 -97 .

    • Search Google Scholar

  • Khurana, E., Singh, U.S. 2001. Ecology of seed and seedling growth for conservation and restoration of tropical dry forest: a review. Environmental Conservation 28 :39–52.

    Singh U.S. , 'Ecology of seed and seedling growth for conservation and restoration of tropical dry forest: a review ' (2001 ) 28 Environmental Conservation : 39 -52 .

    • Search Google Scholar

  • Lowe, L.B., Ayers, G.S., Ries, S.K. 1972. Relationship of seed protein and amino acid composition to seedling vigor and yield of wheat. Agronomy Journal 64 :608–610.

    Ries S.K. , 'Relationship of seed protein and amino acid composition to seedling vigor and yield of wheat ' (1972 ) 64 Agronomy Journal : 608 -610 .

    • Search Google Scholar

  • Lowe, L.B., Ries, S.K. 1972. Effects of environment on the relation between seed protein and seedling vigor in wheat. Canadian Journal of Plant Science 52 :157–164.

    Ries S.K. , 'Effects of environment on the relation between seed protein and seedling vigor in wheat ' (1972 ) 52 Canadian Journal of Plant Science : 157 -164 .

    • Search Google Scholar

  • Lowe, L.B., Ries, S.K. 1973. Endosperm protein of wheat seed as a determinant of seedling growth. Plant Physiology 51 :57–60.

    Ries S.K. , 'Endosperm protein of wheat seed as a determinant of seedling growth ' (1973 ) 51 Plant Physiology : 57 -60 .

    • Search Google Scholar

  • Lupotto, E. 1984. Callus induction and plant regeneration from barley mature embryos. Annals of Botany 54 :523–529.

    Lupotto E. , 'Callus induction and plant regeneration from barley mature embryos ' (1984 ) 54 Annals of Botany : 523 -529 .

    • Search Google Scholar

  • Mathis, R.J., Simpson, E.S. 1986. The interaction of genotype and culture medium on the tissue culture responses in wheat ( Triticum aestivum L. em. Thell) callus. Plant Cell, Tissue and Organ Culture 7 :31–37.

    Simpson E.S. , 'The interaction of genotype and culture medium on the tissue culture responses in wheat (Triticum aestivum L. em. Thell) callus ' (1986 ) 7 Plant Cell, Tissue and Organ Culture : 31 -37 .

    • Search Google Scholar

  • Milberg, P., Lamont, B.B. 1997. Seed/cotyledon size and nutrient content play a major role in early performance of species on nutrient-poor soil. New Phytologist 137 :665–672.

    Lamont B.B. , 'Seed/cotyledon size and nutrient content play a major role in early performance of species on nutrient-poor soil ' (1997 ) 137 New Phytologist : 665 -672 .

    • Search Google Scholar

  • Negi, A.K., Todaria, N.P. 1997. Effect of seed size and weight on germination pattern and seedling development of some multi-purpose tree species of Garhwal Himalaya. Indian Forester 123 :32–36.

    Todaria N.P. , 'Effect of seed size and weight on germination pattern and seedling development of some multi-purpose tree species of Garhwal Himalaya ' (1997 ) 123 Indian Forester : 32 -36 .

    • Search Google Scholar

  • Orton, T.J. 1979. A quantitative analysis of growth and regeneration from tissue cultures Hordeum vulgare, Hordeum jubatum and their interspecific hybrids. Environmental and Experimental Botany 19 :319–335.

    Orton T.J. , 'A quantitative analysis of growth and regeneration from tissue cultures Hordeum vulgare, Hordeum jubatum and their interspecific hybrids ' (1979 ) 19 Environmental and Experimental Botany : 319 -335 .

    • Search Google Scholar

  • Özgen, M., Türet, M., Altinok, S., Sancak, C. 1998. Efficient callus induction and plant regeneration from mature embryo culture of winter wheat ( Triticum aestivum L.) genotypes. Plant Cell Reports 18 :331–335.

    Sancak C. , 'Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes ' (1998 ) 18 Plant Cell Reports : 331 -335 .

    • Search Google Scholar

  • Rengel, Z., Jelaska, S. 1986. Somatic embryogenesis and plant regeneration from seedling tissues of Hordeum vulgare L. J. Plant Physiology 124 :385–392.

    Jelaska S. , 'Somatic embryogenesis and plant regeneration from seedling tissues of Hordeum vulgare L ' (1986 ) 124 J. Plant Physiology : 385 -392 .

    • Search Google Scholar

  • Ries, S.K. 1971. The relationship of size and protein content of bean seed with growth and yield. Proceedings of the American Society of Horticultural Science 96 :557–560.

    Ries S.K. , 'The relationship of size and protein content of bean seed with growth and yield ' (1971 ) 96 Proceedings of the American Society of Horticultural Science : 557 -560 .

    • Search Google Scholar

  • Roach, D.A. 1987. Variation in seed and seedling size in Anthoxanthum odoratum . American Midland Naturalist 90 :493–498.

    Roach D.A. , 'Variation in seed and seedling size in Anthoxanthum odoratum ' (1987 ) 90 American Midland Naturalist : 493 -498 .

    • Search Google Scholar

  • Sayar, M.T., Birsin, M.A., Ulukan, H., Özgen, M. 1999. Effect of seed size on the tissue culture response of callus from mature embryos of wheat species. Wheat Information Service 89 :1–6.

    Özgen M. , 'Effect of seed size on the tissue culture response of callus from mature embryos of wheat species ' (1999 ) 89 Wheat Information Service : 1 -6 .

    • Search Google Scholar

  • Seiwa, K., Kikuzawa, K. 1991. Phenology of tree seedlings in relation to seed size. Canadian Journal of Botany 69 :532–538.

    Kikuzawa K. , 'Phenology of tree seedlings in relation to seed size ' (1991 ) 69 Canadian Journal of Botany : 532 -538 .

    • Search Google Scholar

  • Smart, A.J., Moser, L.E. 1999. Switchgrass seedling development as affected by seed size. Agronomy Journal 91 :335–338.

    Moser L.E. , 'Switchgrass seedling development as affected by seed size ' (1999 ) 91 Agronomy Journal : 335 -338 .

    • Search Google Scholar

  • Snedecor, G.W., Cochran, W.G. 1967. Statistical Methods. The Iowa State University Press, Iowa, USA.

    Cochran W.G. , '', in Statistical Methods , (1967 ) -.

  • Thomas, M.R., Scott, K.J. 1985. Plant regeneration by somatic embryogenesis from callus initiated from immature embryos and immature inflorescences of Hordeum vulgare . Journal of Plant Physiology 121 :159–169.

    Scott K.J. , 'Plant regeneration by somatic embryogenesis from callus initiated from immature embryos and immature inflorescences of Hordeum vulgare ' (1985 ) 121 Journal of Plant Physiology : 159 -169 .

    • Search Google Scholar

  • Tripathi, R.S., Khan, M.L. 1990. Effects of seed weight and microsite characteristics on germination and seedling fitness in two species of Quercus in a subtropical wet hill forest. Oikos 57 :289–296.

    Khan M.L. , 'Effects of seed weight and microsite characteristics on germination and seedling fitness in two species of Quercus in a subtropical wet hill forest ' (1990 ) 57 Oikos : 289 -296 .

    • Search Google Scholar

  • Tuchisawa, M., Enomoto, S., Ohyama, K. 1986. Plant regeneration from callus of immature barley embryos. Japanese Journal of Breeding 36 :28–29.

    Ohyama K. , 'Plant regeneration from callus of immature barley embryos ' (1986 ) 36 Japanese Journal of Breeding : 28 -29 .

    • Search Google Scholar

  • Ward, K.A., Jordan, M.C. 2001. Callus formation and plant regeneration from immature and mature embryos of rye ( Secale cereale L.). In Vitro Cellular and Developmental Biology-Plant 37 :361–368.

    Jordan M.C. , 'Callus formation and plant regeneration from immature and mature embryos of rye (Secale cereale L.) ' (2001 ) 37 In Vitro Cellular and Developmental Biology-Plant : 361 -368 .

    • Search Google Scholar

  • Weigel, R.C., Hughes, K.W. 1985. Long term regeneration by somatic embryogenesis in barley ( Hordeum vulgare L.) tissue cultures derived from apical meristem explants. Plant Cell, Tissue and Organ Culture 5 :151–162.

    Hughes K.W. , 'Long term regeneration by somatic embryogenesis in barley (Hordeum vulgare L.) tissue cultures derived from apical meristem explants ' (1985 ) 5 Plant Cell, Tissue and Organ Culture : 151 -162 .

    • Search Google Scholar

  • Yildiz, M., Özgen, M. 2004. The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax (Linum usitatissimum) . Plant Cell, Tissue and Organ Culture 77 :111–115.

    Özgen M. , 'The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax (Linum usitatissimum) ' (2004 ) 77 Plant Cell, Tissue and Organ Culture : 111 -115 .

    • Search Google Scholar
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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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