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  • 1 Institute of Plant Protection Vasilkovska St., 33 Kiev 03022 Ukraine
  • 2 NAGREF-Cereal Institute Thessaloniki, Hellas Greece
  • 3 Technology, T. E. I. of Kalamata, Antikalamos Plant Production Department, School of Agricultural 24 100 Kalamata, Hellas Greece
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This study was undertaken to investigate the allelic diversity in seed storage proteins in 11 substituted hexaploid triticale cultivars (all spring). These cultivars were developed at the Cereal Institute of Thessaloniki, Hellas, in the 1970s and 1980s, after selection on segregating material originating from International Maize and Wheat Improvement Center (CIMMYT). Seeds from each line were used to determine alleles at the loci for high molecular weight glutenin subunits (HMW-GS) Glu-A1, Glu-B1, Glu-R1 (or Sec-3 ) and gliadins (the loci Gli-A1 and Gli-B1 ). For this to be done, acid polyacrylamide gel electrophoresis for gliadins and SDS-electrophoresis for high molecular weight glutenins (HMW-GS) were applied. Analysis of the electrophoretic patterns obtained from the above-mentioned material revealed that only 5 out of the 11 cultivars were biochemically uniform (cv. ‘Vryto’, ‘Thisvi’, ‘Dada’, ‘Leto’ and ‘Ekate’). On the contrary, the rest of the cultivars, despite they were under seed production process, exhibited heterogeneity. Cv. ‘Dada’, which was found to be uniform, is of special interest, due to its productivity, especially under drought stress conditions.

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