Cereal Research Communications
Volume/Issue:
Volume 35: Issue 4
Expression of the 1D×5 high molecular weight glutenin subunit protein in transgenic rice
Authors:
M. Oszvald

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B. Jenes Agricultural Biotechnology Center Plant Transformation Group Gödöllő Hungary

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S. Tömösközi Budapest University of Technology and Economics Department of Applied Biotechnology and Food Science Budapest Hungary

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F. Békés Plant Industry CSIRO GPO Box 1600 Canberra ACT 2601 Australia

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L. Tamás Eötvös Loránd University Department of Plant Physiology and Molecular Plant Biology Pázmány Péter sétány 1/C H-1117 Budapest Hungary

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Pages:
1543–1549
Online Publication Date:
23 Oct 2007
Publication Date:
01 Dec 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.35.2007.4.1
Keywords:
wheat storage proteins; HMW glutenin expression; functional properties; rice endosperm
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We generated and analysed transgenic rice lines synthesizing substantial amounts of high-molecular-weight glutenin subunit (HMW-GS) from wheat. Particle bombardment has been used to transform rice cultivars ( Orysa sativa L.) with a cassette carrying the gene of 1D×5 HMW glutenin subunit. Twelve independent lines were recovered and PCR results on genomic DNS confirmed the integration of the transgene into it. Five lines set seeds. Seeds were analysed by SDS-PAGE and Western blot and we proved the presence of foreign protein in the starchy endosperm. The amount of 1D×5 HMW-GS in rice endosperm represents 0.75–3.18% of the alcohol soluble proteins. These results are the first example of significantly changing storage protein composition of rice exploiting the method of gene technology. This alteration may have considerable effect on the functional properties, including strength and stability of the dough made of transgenic rice flour.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

 

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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