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  • 1 Budapest University of Technology and Economics Department of Applied Biotechnology and Food Science Budapest Hungary
  • | 2 Agricultural Biotechnology Center Plant Transformation Group Gödöllő Hungary
  • | 3 Plant Industry CSIRO GPO Box 1600 Canberra ACT 2601 Australia
  • | 4 Eötvös Loránd University Department of Plant Physiology and Molecular Plant Biology Pázmány Péter sétány 1/C H-1117 Budapest Hungary
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We generated and analysed transgenic rice lines synthesizing substantial amounts of high-molecular-weight glutenin subunit (HMW-GS) from wheat. Particle bombardment has been used to transform rice cultivars ( Orysa sativa L.) with a cassette carrying the gene of 1D×5 HMW glutenin subunit. Twelve independent lines were recovered and PCR results on genomic DNS confirmed the integration of the transgene into it. Five lines set seeds. Seeds were analysed by SDS-PAGE and Western blot and we proved the presence of foreign protein in the starchy endosperm. The amount of 1D×5 HMW-GS in rice endosperm represents 0.75–3.18% of the alcohol soluble proteins. These results are the first example of significantly changing storage protein composition of rice exploiting the method of gene technology. This alteration may have considerable effect on the functional properties, including strength and stability of the dough made of transgenic rice flour.

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Cereal Research Communications
Language English
Size B5
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
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Springer Nature Switzerland AG
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ISSN 0133-3720 (Print)
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