Cereal Research Communications
Volume/Issue:
Volume 35: Issue 4
Root colonization vs. seedling growth, in two Azospirillum -inoculated wheat species
Authors:
M. Pereyra Unidad Integrada Facultad de Ciencias Agrarias de la Universidad Nacional de Mar del Plata — INTA C. C. 276-INTA 7620 Balcarce Argentina

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R. González Unidad Integrada Facultad de Ciencias Agrarias de la Universidad Nacional de Mar del Plata — INTA C. C. 276-INTA 7620 Balcarce Argentina

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C. Creus Unidad Integrada Facultad de Ciencias Agrarias de la Universidad Nacional de Mar del Plata — INTA C. C. 276-INTA 7620 Balcarce Argentina

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C. Barassi Unidad Integrada Facultad de Ciencias Agrarias de la Universidad Nacional de Mar del Plata — INTA C. C. 276-INTA 7620 Balcarce Argentina

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Pages:
1621–1629
Online Publication Date:
23 Oct 2007
Publication Date:
01 Dec 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.35.2007.4.9
Keywords:
Azospirillum colonization; wheat; seedling growth; growth promotion
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Data evaluating the growth promoting effects of Azospirillum on wheat seedlings according to the inoculum level/root colonization effectiveness (number of bacterial cells), is scarce. Uniform 1-cm size, 72-h old wheat seedlings grown in the dark at 22 °C were inoculated with: i) 10 3 , 10 5 , 10 7 and 10 8A. brasilense cells per T. aestivum cv. ProINTA Federal seedling; ii) 10 2 , 10 5 and 10 8A. brasilense cells per T. durum cv. Buck Topacio seedling; iii) 10 6 heat killed bacteria (HKB) cells per cultivar seedling; iv) phosphate buffer pH 6.8 (NI) as control seedlings for both cultivars. Afterwards, seedling growth proceeded in water in the dark at 22 °C for another 48 h. Alive or dead Azospirillum cells were suspended in phosphate buffer pH 6.8. Root and shoot growth were determined measuring the length and projected area of their digitalized images. When treated with inocula concentrations ranging from 10 2 to 10 5 cells per seedling, both Triticum species reached a maximum level of colonization harboring 10 6 to 10 7 cells per seedling. No differences could be detected between NI and HKB treated seedlings for both Triticum species. Triticum aestivum cv. ProINTA Federal seedlings reached the maximum growth promotion when roots were colonized with a number of bacterial cells ranging from 5 · 10 6 to 1.5 · 10 8 per seedling. Triticum durum cv. Buck Topacio seedlings showed maximum growth promotion when 3.3 · 10 7 cells were present in their roots. Higher values of colonization showed no growth promoting effects with respect to the controls. It may be concluded that in these experimental conditions the optimum inoculum concentration is 5 · 10 5 cells per seedling for both T. aestivum cv. ProINTA Federal and T. durum cv. Buck Topacio.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

 

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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