Six different 1RS-specific molecular markers (RMS13, Bmac213, GPI, 5S, SCM9, IAG95) were tested in twenty rye cultivars of various origin. The aim of the experiments was to choose rye cultivars which give polymorphic PCR products with these 1RS-specific markers compared to the wheat cultivar Mv Magdaléna, which contains the 1BL.1RS translocation. The polymorphic rye cultivars can be presumed to differ from the 1BL.1RS translocation originating from the Petkus rye cultivar and will hopefully carry effective resistance genes which can be incorporated into the 1BL.1RS translocation in wheat. Twenty rye cultivars (at least two plants per cultivar) were analysed with these markers. Of fifty-two rye samples analysed, three plants were found to be polymorphic, one (Kisvárdai Alacsony from Hungary), for the 5S marker, one (Kriszta from Hungary) for the RMS13 marker and one (Porto from Portugal) for the SCM9 marker. The polymorphic plants were grown to maturity in the phytotron.
Bedő, Z., Balla, L., Szunics, L., Láng, L., Kramarik Kissimon, J. 1993. A martonvásári 1B/1R transzlokációs búzafajták agronómiai tulajdonságai. (Agronomic properties of Martonvásár wheat varieties bearing the 1B/1R translocation). Növénytermelés 42 :391–398.
Kramarik Kissimon J. , 'A martonvásári 1B/1R transzlokációs búzafajták agronómiai tulajdonságai ' (1993 ) 42 Növénytermelés : 391 -398 .
Börner, A., Korzun, V. 1998. A consensus linkage map of rye ( Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci. Theor. Appl. Genet. 97 :1279–1288.
Korzun V. , 'A consensus linkage map of rye (Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci ' (1998 ) 97 Theor. Appl. Genet. : 1279 -1288 .
Graybosch, R.A. 2001. Uneasy unions: quality effects of rye chromatin transfers to wheat. J. Cereal Sci. 33 :3–16.
Graybosch R.A. , 'Uneasy unions: quality effects of rye chromatin transfers to wheat ' (2001 ) 33 J. Cereal Sci : 3 -16 .
Hackauf, B., Wehling, P. 2002. Identification of microsatellite polymorphisms in an expressed portion of the rye genome. Plant Breeding 121 :17–25.
Wehling P. , 'Identification of microsatellite polymorphisms in an expressed portion of the rye genome ' (2002 ) 121 Plant Breeding : 17 -25 .
Khlestkina, E.K., Than, M.H.M., Pestsova, E.G., Röder, M.S., Malyshev, S.V., Korzun, V., Börner, A. 2004. Mapping of 99 new microsatellite loci in rye ( Secale cereale L.) including 39 expressed sequence tags. Theor. Appl. Genet. 109 :725–732.
Börner A. , 'Mapping of 99 new microsatellite loci in rye (Secale cereale L.) including 39 expressed sequence tags ' (2004 ) 109 Theor. Appl. Genet. : 725 -732 .
Koebner, R.M.D. 1995. Generation of PCR-based markers for detection of rye chromatin in a wheat background. Theor. Appl. Genet. 90 :740–745.
Koebner R.M.D. , 'Generation of PCR-based markers for detection of rye chromatin in a wheat background ' (1995 ) 90 Theor. Appl. Genet. : 740 -745 .
Korzun, V., Malyshev, S., Kartel, N., Westermann, T., Weber, W.E., Börner, A. 1998. A genetic linkage map of rye ( Secale cereale L.). Theor. Appl. Genet. 96 :203–208.
Börner A. , 'A genetic linkage map of rye (Secale cereale L.) ' (1998 ) 96 Theor. Appl. Genet. : 203 -208 .
Korzun, V., Malyshev, S., Voylokov, A.V., Börner, A. 2001. A genetic map of rye ( Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci. Theor. Appl. Genet. 102 :709–717.
Börner A. , 'A genetic map of rye (Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci ' (2001 ) 102 Theor. Appl. Genet. : 709 -717 .
Kotvics, G., Krisztián, J., Dornbach, L. 1999. Perennial cultivated rye; a new variety for Hungarian crop production. Agrofórum X. 5.63.
Kruppa, J. 2001. Results obtained in the breedup and production of rye and triticale. PhD Thesis, Department of Agriculture, Centre of Agricultural Sciences, University of Debrecen.
Kőszegi, B., Linc, G., Juhász, A., Láng, L., Molnár-Láng, M. 2000. Occurrence of the 1RL/1BL wheat-rye translocation in Hungarian wheat varieties. Acta Agron. Hung. 48 :227–236.
Molnár-Láng M. , 'Occurrence of the 1RL/1BL wheat-rye translocation in Hungarian wheat varieties ' (2000 ) 48 Acta Agron. Hung. : 227 -236 .
Ma, X.F., Wanous, M.K., Houchins, K., Rodriguez Milla, M. A., Goicoechea, P.G., Wang, Z., Xie, M., Gustafson, J.P. 2001. Molecular linkage mapping in rye ( Secale cereale L.). Theor. Appl. Genet. 102 :517–523.
Gustafson J.P. , 'Molecular linkage mapping in rye (Secale cereale L.) ' (2001 ) 102 Theor. Appl. Genet. : 517 -523 .
Mago, R., Spielmeyer, W., Lawrence, G., Lagudah, E., Ellis, J., Pryor, A. 2002. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor. Appl. Genet. 104 :1317–1324.
Pryor A. , 'Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines ' (2002 ) 104 Theor. Appl. Genet. : 1317 -1324 .
Masojc, P., Myskow, B., Milczarski, P. 2001. Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor. Appl. Genet. 102 :1273–1279.
Milczarski P. , 'Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers ' (2001 ) 102 Theor. Appl. Genet. : 1273 -1279 .
McIntosh, R.A., Yamazaki, Y., Devos, K.M., Dubcovsky, J., Rogers, W.J., Appels, R. 2003. Catalogue of gene symbols for wheat. In: Pogna, N.E., Romano, M., Pogna, E.A., Galterio, G. (eds), Proc. 10 th Intern. Wheat Genet. Symp. Vol. 4, Paestum, Italy, pp. 34.
Mohler, V., Hsam, S.L.K., Zeller, F.J., Wenzel, G. 2001. An STS marker distinguishing the rye-derived powdery mildew resistance alleles at the Pm8/Pm17 locus of common wheat. Plant Breeding 120 :448–450.
Wenzel G. , 'An STS marker distinguishing the rye-derived powdery mildew resistance alleles at the Pm8/Pm17 locus of common wheat ' (2001 ) 120 Plant Breeding : 448 -450 .
Molnár-Láng, M., Linc, G., Sutka, J. 1996. Transfer of the recessive crossability allele kr1 from Chinese Spring into winter wheat variety Martonvásári 9. Euphytica 90 :301–305.
Sutka J. , 'Transfer of the recessive crossability allele kr1 from Chinese Spring into winter wheat variety Martonvásári 9 ' (1996 ) 90 Euphytica : 301 -305 .
Nagy, E.D., Christoph, E., Molnár-Láng, M., Lelley, T. 2003a. Genetic mapping of sequence-specific PCR-based markers on the short arm of the 1BL.1RS wheat rye translocation. Euphytica 132 :243–250.
Lelley T. , 'Genetic mapping of sequence-specific PCR-based markers on the short arm of the 1BL.1RS wheat rye translocation ' (2003 ) 132 Euphytica : 243 -250 .
Nagy, E.D., Lelley, T. 2003b. Genetic and physical mapping of sequence specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm in a wheat background. Theor. Appl. Genet. 107 :1271–1277.
Lelley T. , 'Genetic and physical mapping of sequence specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm in a wheat background ' (2003 ) 107 Theor. Appl. Genet. : 1271 -1277 .
Nagy, E.D., Molnár-Láng, M. 2000. Frequency of pairing between the 1B/1R translocation and its respective homo(eo)logues in a wheat-rye hybrid as revealed by GISH. Cereal Res. Comm. 28 :41–48.
Molnár-Láng M. , 'Frequency of pairing between the 1B/1R translocation and its respective homo(eo)logues in a wheat-rye hybrid as revealed by GISH ' (2000 ) 28 Cereal Res. Comm. : 41 -48 .
Rajaram, S., Mann, C.E., Oniz-Ferrara, G., Mujeeb-Kazi, A. 1983. Adaptation, stability and high yield potential of certain 1B/1R CIMMYT wheats. In: Sakamoto, S. (ed.), Proc. of the 6 th Intern. Wheat Genet. Symp., Kyoto, Japan, pp. 613–621.
Ramsay, L., Macaulay, M., degli Ivanissevich, S., MacLean, K., Cardle, L., Fuller, J., Edwards, K.J., Tuvesson, S., Morgante, M., Massari, A., Maestri, E., Marmiroli, N., Sjakste, T., Ganal, M., Powell, W., Waugh, R. 2000. A simple sequence repeat-based linkage map of barley. Genetics 156 :1997–2005.
Waugh R. , 'A simple sequence repeat-based linkage map of barley ' (2000 ) 156 Genetics : 1997 -2005 .
Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149 :2007–2023.
Ganal M.W. , 'A microsatellite map of wheat ' (1998 ) 149 Genetics : 2007 -2023 .
Saal, B., Wricke, G. 1999. Development of simple sequence repeat markers in rye ( Secale cereale L.). Genome 42 :964–972.
Wricke G. , 'Development of simple sequence repeat markers in rye (Secale cereale L.) ' (1999 ) 42 Genome : 964 -972 .
Schlegel, R., Korzun, V. 1997. About the origin of 1RS.1BL wheat-rye chromosome translocations from Germany. Plant Breed. 116 :537–540.
Korzun V. , 'About the origin of 1RS.1BL wheat-rye chromosome translocations from Germany ' (1997 ) 116 Plant Breed : 537 -540 .
Shepherd, K.W. 1973. Homeology of wheat and alien chromosomes controlling endosperm protein phenotypes. In: Sears, E.R., Sears, L.M.S. (eds), Proc. 4 th Intern. Wheat Genet. Symp., Univ, Missouri, Columbia, USA, pp. 745–760.
Shepherd K.W. , '', in Proc. 4th Intern. Wheat Genet. Symp. , (1973 ) -.
van Capenhout, S. 1997. Chromosome-specific PCR markers for wheat genome analysis and manipulation. Dissertationes de Agricultura 337. Katholike Universität, Leuven
Capenhout S. , '', in Chromosome-specific PCR markers for wheat genome analysis and manipulation. Dissertationes de Agricultura 337 , (1997 ) -.
Zeller, F.J. 1973. 1B/1R wheat-rye chromosome substitutions and translocations. In: Sears, E.R., Sears, L.M.S. (eds), Proc. 4 th Intern. Wheat Genet. Symp., Univ, Missouri, Columbia, USA, pp. 209–221.
Zeller F.J. , '', in Proc. 4th Intern. Wheat Genet. Symp. , (1973 ) -.
Zeller, F.J., Fuchs, E. 1983. Cytologie und Krankheitresistenz einer 1A/1R und mehrerer 1B/1R Weizen-Roggen Translokationssorten. Z. Pflanzenzüchtung 90 :285–296.
Fuchs E. , 'Cytologie und Krankheitresistenz einer 1A/1R und mehrerer 1B/1R Weizen-Roggen Translokationssorten ' (1983 ) 90 Z. Pflanzenzüchtung : 285 -296 .
Zeller, F.J., Hsam, F.L.K. 1984. Broadening the genetic variability of cultivated wheat by utilizing rye chromatin. In: Sakamoto, S. (ed.), Proc. 6 th Intern. Wheat Genet. Symp., Kyoto, Japan, pp. 161–173.