Cereal Research Communications
Volume/Issue:
Volume 36: Issue 1
Polyclonal antibodies to grain gliadins and their application in wheat quality prediction
Authors: Q. Li, K. Ji 3 , X. Song 1 , E. Zhang 1 , Y. Pei 1 , and Y. Yan 3
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  • 1 Qingdao Agricultural University Laboratory of Protein Biochemistry, College of Life Science Qingdao 266109 China
  • 2 Shandong Academy of Agricultural Sciences Institute of Vegetable Research Jinan 250100 Shandong Province China
  • 3 Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science Beijing 100037 China
DOI:
https://doi.org/10.1556/crc.36.2008.1.12
Page Count:
117–124
Publication Date:
01 Mar 2008
Online Publication Date:
18 Feb 2008
Article Category:
Research Article
Restricted access

Gliadins were extracted from three wheat varieties, viz. Nongda 99260037 (good quality), Henan 9023 (media quality) and Nongda 98123 (poor quality), and α-, β-, γ-, ω -gliadins were purified from Nongda 99260037 using preparative SDS-PAGE. The total gliadins and α-, β-, γ-, ω -gliadins were used as antigens to immunise BALB/C mice, and the corresponding polyclonal antibodies were prepared, designated as Anti-A, Anti-B, Anti-C, Anti-A α , Anti-A β , Anti-A γ and Anti-A ω , respectively. Binding of the polyclonal antibodies with 8 varieties, that varied in quality properties, showed correlations with some wheat quality parameters. Correlation coefficients between antibodies against total gliadins or γ -, and ω -gliadin of Nongda 99260037 and quality parameters were higher than other antibodies. Of seven polyclonal antibodies tested, three (Anti-A γ , Anti-A ω and Anti-A) displayed significant positive or negative associations between antibody binding and dough development time, strength, valorimenter value and stability time, but no significant correlations were observed with water absorption. These results suggest that polyclonal antibodies could be used for rapid prediction and screening of wheat quality parameters.

  • Andrews, J.L., Blundell, M.J., Skerritt, J.H. 1993. A simple antibody-based test for dough strength. III. Further simplification and collaborative evaluation for wheat quality screening. Cereal Chem. 70 :241–246.

    Skerritt J.H. , 'A simple antibody-based test for dough strength. III. Further simplification and collaborative evaluation for wheat quality screening ' (1993 ) 70 Cereal Chem. : 241 -246.

    • Search Google Scholar

  • Andrews, J.L., Skerritt, J.H. 1996. Wheat dough extensibility screening using a two-site enzyme-linked immunosorbent assay (ELISA) with antibodies to low molecular weight glutenin subunits. Cereal Chem. 73 :650–657.

    Skerritt J.H. , 'Wheat dough extensibility screening using a two-site enzyme-linked immunosorbent assay (ELISA) with antibodies to low molecular weight glutenin subunits ' (1996 ) 73 Cereal Chem. : 650 -657.

    • Search Google Scholar

  • Belton, P.S. 1999. On the elasticity of wheat gluten. J. Cereal Sci., 29 :103–107.

    Belton P.S. , 'On the elasticity of wheat gluten ' (1999 ) 29 J. Cereal Sci. : 103 -107.

  • Branlard, G., Dardevet, M. 1985. Diversity of grain proteins and bread wheat quality. I. Correlation between gliadin bands and flour quality characteristics. J. Cereal Sci. 3 :329–343.

    Dardevet M. , 'Diversity of grain proteins and bread wheat quality. I. Correlation between gliadin bands and flour quality characteristics ' (1985 ) 3 J. Cereal Sci. : 329 -343.

    • Search Google Scholar

  • Brett, G..M., Mills, E.N.C., Tatham, A.S., Fido, R.J., Shewry, P.R., Morgan, M.R.A. 1993. Immunochemical identification of LMW subunits of glutenin associated with bread-making quality of wheat flours. Theor. Appl. Genet. 86 :442–448.

    Morgan M.R.A. , 'Immunochemical identification of LMW subunits of glutenin associated with bread-making quality of wheat flours ' (1993 ) 86 Theor. Appl. Genet. : 442 -448.

    • Search Google Scholar

  • Feeney, K.A., Tatham, A.S., Gilbert, S.M., Fido, R.J., Halford, N.G., Shewry, P.R. 2001. Synthesis, expression and characterization of peptides comprised of perfect repeat motifs based on a wheat seed storage protein. Biochem. Biophys. Acta 1546 :346–355.

    Shewry P.R. , 'Synthesis, expression and characterization of peptides comprised of perfect repeat motifs based on a wheat seed storage protein ' (2001 ) 1546 Biochem. Biophys. Acta : 346 -355.

    • Search Google Scholar

  • Festenstein, G..N., Hay, F.C., Shewry, P.R. 1987. Immunological relationship of the prolamin storage protein of barley, wheat, rye and oat. Biochem. Biophys. Acta 912 :317–383.

    Shewry P.R. , 'Immunological relationship of the prolamin storage protein of barley, wheat, rye and oat ' (1987 ) 912 Biochem. Biophys. Acta : 317 -383.

    • Search Google Scholar

  • Fido, R.J., Bekest, F., Grast, P.W., Tatham, A.S. 1997. Effects of α-, β-, γ- and ω -gliadins on the dough mixing properties of wheat flour. J. Cereal Sci. 26 :271–277.

    Tatham A.S. , 'Effects of α-, β-, γ- and ω-gliadins on the dough mixing properties of wheat flour ' (1997 ) 26 J. Cereal Sci. : 271 -277.

    • Search Google Scholar

  • Flate, N.E.S., Uhlen, A.K. 2003. Association between allelic variation at the combined Gli-1, Gli-3 loci and protein quality in common wheat ( Triticum aestivum L.). J. Cereal Sci. 37 :129–137.

    Uhlen A.K. , 'Association between allelic variation at the combined Gli-1, Gli-3 loci and protein quality in common wheat (Triticum aestivum L.) ' (2003 ) 37 J. Cereal Sci. : 129 -137.

    • Search Google Scholar

  • Fritschy, F., Windemann, H., Baumgartner, E. 1985. Quantitative of wheat gliadins in foods by enzyme linked immunosorbent assay. Z. Lebensm. Uniters Forsch., 181 :379–385.

    Baumgartner E. , 'Quantitative of wheat gliadins in foods by enzyme linked immunosorbent assay ' (1985 ) 181 Z. Lebensm. Uniters Forsch. : 379 -385.

    • Search Google Scholar

  • Hill, A.S., Giersch, T.M., Loh, C.S., Skerritt, J.H. 1999. Immunoassay for wheat processing quality: Utilization of a sandwich assay incorporating an immobilized single-chain fragment. J. Agric. Food Chem. 47 :4484–4490.

    Skerritt J.H. , 'Immunoassay for wheat processing quality: Utilization of a sandwich assay incorporating an immobilized single-chain fragment ' (1999 ) 47 J. Agric. Food Chem. : 4484 -4490.

    • Search Google Scholar

  • Khatkar, B.S., Fidot, R.J., Tatham, A.S., Schofield, J.D. 2002. Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality. J. Cereal Sci. 3 :299–306.

    Schofield J.D. , 'Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality ' (2002 ) 3 J. Cereal Sci. : 299 -306.

    • Search Google Scholar

  • MacRitchie, F. 1984. Baking quality of wheat flours. Adv. Food Res. 29 :201–277.

    MacRitchie F. , 'Baking quality of wheat flours ' (1984 ) 29 Adv. Food Res. : 201 -277.

  • MacRitchie, F. 1987. Evaluation of contributions from wheat protein fractions to dough mixing and bread-making. J. Cereal Sci. 6 :259–268.

    MacRitchie F. , 'Evaluation of contributions from wheat protein fractions to dough mixing and bread-making ' (1987 ) 6 J. Cereal Sci. : 259 -268.

    • Search Google Scholar

  • MacRitchie, F., DuCros, D.L., Wrigley, C.W. 1990. Flour polypeptides related to wheat quality. Adv. Cereal Sci. Tech. 10 :79–145.

    Wrigley C.W. , 'Flour polypeptides related to wheat quality ' (1990 ) 10 Adv. Cereal Sci. Tech. : 79 -145.

    • Search Google Scholar

  • Martinez, Ma del C., Ruiz, M., Carrillo, J.M. 2005. Effects of different prolamin alleles on durum wheat quality properties. J. Cereal Sci. 41 :123–131.

    Carrillo J.M. , 'Effects of different prolamin alleles on durum wheat quality properties ' (2005 ) 41 J. Cereal Sci. : 123 -131.

    • Search Google Scholar

  • Metakovsky, E.V., Annicchiarico, P., Boggini, G., Pogna, N.E. 1997a. Relationships between gliadin alleles and dough strength in Italian common wheats. J. Cereal Sci. 25 :229–236.

    Pogna N.E. , 'Relationships between gliadin alleles and dough strength in Italian common wheats ' (1997 ) 25 J. Cereal Sci. : 229 -236.

    • Search Google Scholar

  • Metakovsky, E.V., Felix, I., Branlard, G. 1997b. Association between dough quality (W value) and certain gliadin alleles in French common wheat cultivars. J. Cereal Sci. 26 :371–373.

    Branlard G. , 'Association between dough quality (W value) and certain gliadin alleles in French common wheat cultivars ' (1997 ) 26 J. Cereal Sci. : 371 -373.

    • Search Google Scholar

  • Payne, P.I., Seekings, J.A., Worland, A.J., Jarvis, M.G., Holt, L.M. 1987. Allelic variation of glutenin subunits and gliadins and its effect on bread-making quality in wheat: analysis of F5 progeny from Chinese Spring × Ch. Spring (Hope 1A). J. Cereal Sci. 6 :103–118.

    Holt L.M. , 'Allelic variation of glutenin subunits and gliadins and its effect on bread-making quality in wheat: analysis of F5 progeny from Chinese Spring × Ch. Spring (Hope 1A) ' (1987 ) 6 J. Cereal Sci. : 103 -118.

    • Search Google Scholar

  • Pogna, N.E., Boggini, G., Corbellini, M., Cattaneo, M., Peruffo, A.D.B. 1982. Association between gliadin electrophoretic bands and quality in common wheat. Can. J. Plant Sci. 4 :913–918.

    Peruffo A.D.B. , 'Association between gliadin electrophoretic bands and quality in common wheat ' (1982 ) 4 Can. J. Plant Sci. : 913 -918.

    • Search Google Scholar

  • Prabhasankar, P. 2002. Electrophoretic and immunochemical characteristics of wheat protein fractions and their relationship to chapati-making quality. Food Chem. 78 :81–87.

    Prabhasankar P. , 'Electrophoretic and immunochemical characteristics of wheat protein fractions and their relationship to chapati-making quality ' (2002 ) 78 Food Chem. : 81 -87.

    • Search Google Scholar

  • Skerritt, J.H. 1991a. A simple antibody-based test for dough strength. I. Development of the method and choice of antibodies. Cereal Chem. 68 :467–474.

    Skerritt J.H. , 'A simple antibody-based test for dough strength. I. Development of the method and choice of antibodies ' (1991 ) 68 Cereal Chem. : 467 -474.

    • Search Google Scholar

  • Skerritt, J.H. 1991b. Asimple antibody-based test for dough strength. II. Genotype and environmental effects. Cereal Chem. 68 :475–481.

    Skerritt J.H. , 'Asimple antibody-based test for dough strength. II. Genotype and environmental effects ' (1991 ) 68 Cereal Chem. : 475 -481.

    • Search Google Scholar

  • Song, X.Y., Cai, Q.F., Gao, W.S., Liu, G..T., Huang, T.C. 1998. Immunochemical assay for quality and immunological homology of storage proteins in different closely related genera and species of wheat. Acta Bot. Sin. 40 :527–533.

    Huang T.C. , 'Immunochemical assay for quality and immunological homology of storage proteins in different closely related genera and species of wheat ' (1998 ) 40 Acta Bot. Sin. : 527 -533.

    • Search Google Scholar

  • Stenram, U., Heneen, W.K., Skerritt, J.H. 1991. Immunocytochemical localization of wheat storage protein in endosperm cells 30 days after anthesis. J. Exp. Bot. 42 :1347–1355.

  • Van Lonkhuijsen, H.J., Hamer, R.J., Schrender, C. 1992. Influence of specific gliadins on the bread making quality of wheat. Cereal Chem. 69 :174–177.

    Schrender C. , 'Influence of specific gliadins on the bread making quality of wheat ' (1992 ) 69 Cereal Chem. : 174 -177.

    • Search Google Scholar
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
polyclonal antibodies; ELISA; gliadins; wheat quality

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