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  • 1 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Corrensstrasse 3 D-06466 Gatersleben Germany
  • | 2 Heinrich Heine University Institute for Plant Developmental & Molecular Biology Universitätsstr. 1 D-40225 Düsseldorf Germany
  • | 3 Lochow-Petkus GmbH PF 1197 D-29296 Bergen Germany
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PCR assays specific for the GA insensitive dwarfing genes (alleles) Rht-B1b and Rht-D1b were employed to study a series of additional alleles of Rht-B1 and Rht-D1 . The amplification profiles of Rht-B1b and Rht-B1d were not distinguishable from one another, whereas lines carrying Rht-B1c, Rht-B1e and Rht-B1f amplified a product analogous to that of the wild type. At the 4D locus, no discrimination was possible between Rht-D1b, Rht-D1c and Rht-D1d . As a result, the utilisation of these PCR assays is limited. Examples of the analysis of germplasm and spontaneous occurring off-types are presented.

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Cereal Research Communications
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