The present study was focused on the selection of S. cereale cultivars of different geographic origin showing polymorphism detectable by fluorescent in situ hybridization on their 1RS chromosome arms. One perennial and four annual genotypes were tested. FISH was carried out with the DNA probes pSc119.2 and (AAC) 5 . The pSc119.2 probe gave hybridization signals different from that of the rye ‘Petkus’ on the 1RS arms of all five rye cultivars examined. Differences were manifested mainly in the intensity of the labelling, but the complete lack of FISH signals and double signals were also observed.
Alkhimova, O.G., Mazurok, N.A., Potapova, T.A., Zakian, S.M., Heslop-Harrison, J.S., Vershinin, A.V. 2004. Diverse patterns of the tandem repeats organization in rye chromosomes. Chromosoma 113 :42–52.
Vershinin A.V. , 'Diverse patterns of the tandem repeats organization in rye chromosomes ' (2004 ) 113 Chromosoma : 42 -52 .
Bedbrook, J.R., Jones, J., O’Dell, M., Thompson, R.J., Flavell, R.B. 1980. A molecular description of telomeric heterochromatin in Secale species. Cell 19 :545–560.
Flavell R.B. , 'A molecular description of telomeric heterochromatin in Secale species ' (1980 ) 19 Cell : 545 -560 .
Contento, A., Heslop-Harrison, J.S., Schwarzacher, T. 2005. Diversity of a major repetitive DNA sequence in diploid and polyploid Triticeae . Cytogenetic and Genome Research 109 :34–42.
Schwarzacher T. , 'Diversity of a major repetitive DNA sequence in diploid and polyploid Triticeae ' (2005 ) 109 Cytogenetic and Genome Research : 34 -42 .
Cuadrado, A., Jouve, N. 1994. Mapping and organization of highly-repeated DNA sequences by means of simultaneous and sequential FISH and C-banding in 6×-triticale. Chromosome Res. 2 :331–338.
Jouve N. , 'Mapping and organization of highly-repeated DNA sequences by means of simultaneous and sequential FISH and C-banding in 6×-triticale ' (1994 ) 2 Chromosome Res. : 331 -338 .
Cuadrado, A., Jouve, N. 1997. Distribution of highly repeated DNA sequences in species of the genus Secale . Genome 40 :309–317.
Jouve N. , 'Distribution of highly repeated DNA sequences in species of the genus Secale ' (1997 ) 40 Genome : 309 -317 .
Cuadrado, A., Jouve, N. 2002. Evolutionary trends of different repetitive DNA sequences during speciation in the genus Secale. J. Hered. 93 :339–345.
Jouve N. , 'Evolutionary trends of different repetitive DNA sequences during speciation in the genus Secale ' (2002 ) 93 J. Hered. : 339 -345 .
Cuadrado, A., Ceoloni, C., Jouve, N. 1995. Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization. Genome 38 :1061–1069.
Jouve N. , 'Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization ' (1995 ) 38 Genome : 1061 -1069 .
Cuadrado, A., Schwarzacher, T., Jouve, N. 2000. Identification of different chromatin classes in wheat using in situ hybridization with simple sequence repeat oligonucleotides. Theor. Appl. Genet. 101 :711–717.
Jouve N. , 'Identification of different chromatin classes in wheat using in situ hybridization with simple sequence repeat oligonucleotides ' (2000 ) 101 Theor. Appl. Genet. : 711 -717 .
Devos, K.M., Atkinson, M.D., Chinoy, C.N., Francis, H.A., Harcourt, R.L., Koebner, R.M.D., Liu, C.J., Masoj, P., Xie, D.X., Gale, M.D. 1993. Chromosomal rearrangements in the rye genome relative to that of wheat. Theor. Appl. Genet. 85 :673–680.
Gale M.D. , 'Chromosomal rearrangements in the rye genome relative to that of wheat ' (1993 ) 85 Theor. Appl. Genet. : 673 -680 .
Graybosch, R.A. 2001. Uneasy unions: quality effects of rye chromatin transfers to wheat. J. Cereal Sci. 33 :3–16.
Graybosch R.A. , 'Uneasy unions: quality effects of rye chromatin transfers to wheat ' (2001 ) 33 J. Cereal Sci. : 3 -16 .
Heslop-Harrison, J.S. 2000. Comparative genome organization in plants: from sequence and markers to chromatin and chromosomes. Plant Cell 12 :617–635.
Heslop-Harrison J.S. , 'Comparative genome organization in plants: from sequence and markers to chromatin and chromosomes ' (2000 ) 12 Plant Cell : 617 -635 .
Jiang, J., Friebe, B., Gill, B.S. 1994. Chromosome painting of ‘Amigo’ wheat. Theor. Appl. Genet. 89 :811–813.
Gill B.S. , 'Chromosome painting of ‘Amigo’ wheat ' (1994 ) 89 Theor. Appl. Genet. : 811 -813 .
Jones, J.D.G., Flavell, R.B. 1982. The structure, amount and chromosomal localization of defined repeated DNA sequences in species of the genus Secale . Chromosoma 86 :613–641.
Flavell R.B. , 'The structure, amount and chromosomal localization of defined repeated DNA sequences in species of the genus Secale ' (1982 ) 86 Chromosoma : 613 -641 .
Kotvics, G., Krisztián, J., Dornbach, L. 1999. Perennial rye: a novel variety released from a new interspecific hybrid rye. Gyakorlati Agrofórum 10 :63.
Dornbach L. , 'Perennial rye: a novel variety released from a new interspecific hybrid rye ' (1999 ) 10 Gyakorlati Agrofórum : 63 -.
Kőszegi, B., Linc, G., Juhász, A., Láng, L., Molnár-Láng, M. 2000. Occurrence of the 1RL/1BL wheat-rye translocation in Hungarian wheat varieties. Acta Agron. Hung. 48 :227–236.
Molnár-Láng M. , 'Occurrence of the 1RL/1BL wheat-rye translocation in Hungarian wheat varieties ' (2000 ) 48 Acta Agron. Hung. : 227 -236 .
Kruppa, J. 2001. Results obtained in the breedup and production of rye and triticale. Thesis, Department of Agriculture, Centre of Agricultural Sciences, University of Debrecen, pp. 80–84.
Leitch, I.J., Leitch, A.R., Heslop-Harrison, J.S. 1991. Physical mapping of plant DNA sequences by simultaneous in situ hybridization of two differently labelled fluorescent probes. Genome 34 :329–333.
Heslop-Harrison J.S. , 'Physical mapping of plant DNA sequences by simultaneous in situ hybridization of two differently labelled fluorescent probes ' (1991 ) 34 Genome : 329 -333 .
Lelley, T., Eder, C., Grausgruber, H. 2004. Influence of 1BL. 1RS wheat-rye chromosome translocation on genotype by environment interaction. J. Cereal Sci. 39 :313–320.
Grausgruber H. , 'Influence of 1BL. 1RS wheat-rye chromosome translocation on genotype by environment interaction ' (2004 ) 39 J. Cereal Sci. : 313 -320 .
Linc, G., Friebe, B.R., Kynast, R.G., Molnár-Láng, M., Kőszegi, B., Sutka, J., Gill, B.S. 1999. Molecular cytogenetic analysis of Aegilops cylindrica Host. Genome 42 :497–503.
Gill B.S. , 'Molecular cytogenetic analysis of Aegilops cylindrica Host ' (1999 ) 42 Genome : 497 -503 .
Lutz, J., Limpert, E., Bartos, P., Zeller, F.J. 1992. Identification of powdery mildew resistance genes in common wheat ( Triticum aestivum L.). I. Czechoslovakian cultivars. Plant Breed. 108 :33–39.
Zeller F.J. , 'Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). I. Czechoslovakian cultivars ' (1992 ) 108 Plant Breed : 33 -39 .
Marais, G.F., Horn, M., Du Torr, F. 1994. Intergeneric transfer (rye to wheat) of a gene(s) for Russian wheat aphid resistance. Plant Breed. 113 :265–271.
Torr F. , 'Intergeneric transfer (rye to wheat) of a gene(s) for Russian wheat aphid resistance ' (1994 ) 113 Plant Breed : 265 -271 .
Martin, D.J., Stewart, B.G. 1990. Dough stickiness in rye-derived wheat cultivars. Euphytica 51 :77–86.
Stewart B.G. , 'Dough stickiness in rye-derived wheat cultivars ' (1990 ) 51 Euphytica : 77 -86 .
McIntosh, R.A. 1988. Catalogue of gene symbols for wheat. In: Miller, T.E., Koebner, R.M.D. (eds), Proc. 7 th Intern. Wheat Genet. Symp., Cambridge, England, pp. 1225–1323.
McIntyre, C.L., Pereira, S., Moran, L.B., Appels, R. 1990. New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33 :635–640.
Appels R. , 'New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat ' (1990 ) 33 Genome : 635 -640 .
Mettin, D., Bluthner, W.D., Schlegel, G. 1973. Additional evidence on spontaneous 1B/1R wheat rye substitutions and translocations. In: Sears, E.R., Sears, L.M.S. (eds), Proc. 4 th Int. Wheat Genet. Symp., Aug. 6–11, 1973. Agric. Expt. Stn., College of Agric. University of Missouri, Missouri, Columbia, Missouri, pp. 179–184.
Nagy, E.D., Lelley, T. 2003. Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background. Theor. Appl. Genet. 107 :1271–1277.
Lelley T. , 'Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background ' (2003 ) 107 Theor. Appl. Genet. : 1271 -1277 .
Nagy, E.D., Molnár-Láng, M. 2000. Frequency of pairing between the 1B/1R translocations and its respective homo(eo)logues in a wheat-rye hybrid as revealed by GISH. Cereal Res. Comm. 28 :41–48.
Molnár-Láng M. , 'Frequency of pairing between the 1B/1R translocations and its respective homo(eo)logues in a wheat-rye hybrid as revealed by GISH ' (2000 ) 28 Cereal Res. Comm. : 41 -48 .
Naranjo, T., Fernández-Rueda, P. 1991. Homoeology of rye chromosomes to wheat. Theor. Appl. Genet. 82 :577–586.
Fernández-Rueda P. , 'Homoeology of rye chromosomes to wheat ' (1991 ) 82 Theor. Appl. Genet. : 577 -586 .
Schlegel, R., Korzun, V. 1997. About the origin of 1RS. 1BL wheat-rye chromosome translocations from Germany. Plant Breeding 116 :537–540.
Korzun V. , 'About the origin of 1RS. 1BL wheat-rye chromosome translocations from Germany ' (1997 ) 116 Plant Breeding : 537 -540 .
Shepherd, K.W. 1973. Homeology of wheat and alien chromosomes controlling endosperm protein phenotypes. In: Sears, E.R., Sears, L.M.S. (eds), Proc. 4 th Int. Wheat Genet. Symp., Columbia, USA, pp. 745–760.
Singh, N.K., Shepherd, K.W., McIntosh, R.A. 1990. Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R. Theor. Appl. Genet. 80 :609–616.
McIntosh R.A. , 'Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R ' (1990 ) 80 Theor. Appl. Genet. : 609 -616 .
Szakács, É., Linc, G., Láng, L., Molnár-Láng, M. 2004. Detection of the 1A/1R and 1B/1R wheat/rye translocation in new Martonvásár wheat varieties and advanced lines using in situ hybridization. Növénytermelés 53 :527–534.
Molnár-Láng M. , 'Detection of the 1A/1R and 1B/1R wheat/rye translocation in new Martonvásár wheat varieties and advanced lines using in situ hybridization ' (2004 ) 53 Növénytermelés : 527 -534 .
Vershinin, A.V., Alkhimova, E.G., Heslop-Harrison, J.S. 1996. Molecular diversification of tandemly organized DNA sequences and heterochromatic chromosome regions in some Triticeae species. Chromosome Res. 4 :517–525.
Heslop-Harrison J.S. , 'Molecular diversification of tandemly organized DNA sequences and heterochromatic chromosome regions in some Triticeae species ' (1996 ) 4 Chromosome Res. : 517 -525 .
Villareal, R.L., Mujeeb-Kazi, A., Rajaram, S., del Toro, E. 1994. Associated effects of chromosome 1B/1R translocation on agronomic traits in hexaploid wheat. Breed. Sci. 44 :7–11.
Toro E. , 'Associated effects of chromosome 1B/1R translocation on agronomic traits in hexaploid wheat ' (1994 ) 44 Breed. Sci. : 7 -11 .
Vrána, J., Kubaláková, M., Simková, H., Cíhalíková, J., Lysák, M.A., Dolezel, J. 2000. Flow sorting of mitotic chromosomes in common wheat ( Triticum aestivum L.). Genetics 156 :2033–2041.
Dolezel J. , 'Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) ' (2000 ) 156 Genetics : 2033 -2041 .
Zeller, F.J. 1973. 1B/1R wheat-rye chromosome substitutions and translocations. Proc. 4 th Int. Wheat Genet. Symp., Univ, Missouri, Columbia, USA, pp. 209–221.
Zeller, F.J., Fuchs, E. 1983. Cytologie und Krankheitsresistenz einer 1A/1R und mehrerer 1B/1R Weizen-Roggen Translokationssorten. Z. Pflanzenzüchtung 90 :285–296.
Fuchs E. , 'Cytologie und Krankheitsresistenz einer 1A/1R und mehrerer 1B/1R Weizen-Roggen Translokationssorten ' (1983 ) 90 Z. Pflanzenzüchtung : 285 -296 .
Zeller, F.J., Hsam, F.L.K. 1984. Broadening the genetic variability of cultivated wheat by utilizing rye chromatin. In: Sakamoto, S. (ed.), Proc. 6 th Int. Wheat Genet. Symp., Kyoto, Japan, pp. 161–173.