Cereal Research Communications
Volume/Issue:
Volume 36: Issue 4
Isolation and characterization of a novel HMW-GS Glu-Dx allele from Tibet bread wheat landrace
Authors:
Z. Yan Sichuan Agricultural University Triticeae Research Institute Dujiangyan City 611830 Sichuan China

Search for other papers by Z. Yan in
Current site
Google Scholar
PubMed Close
,
S. Dai Sichuan Agricultural University Triticeae Research Institute Dujiangyan City 611830 Sichuan China

Search for other papers by S. Dai in
Current site
Google Scholar
PubMed Close
,
D. Liu Sichuan Agricultural University Triticeae Research Institute Dujiangyan City 611830 Sichuan China

Search for other papers by D. Liu in
Current site
Google Scholar
PubMed Close
,
Y. Wei Sichuan Agricultural University Triticeae Research Institute Dujiangyan City 611830 Sichuan China

Search for other papers by Y. Wei in
Current site
Google Scholar
PubMed Close
, and
Y. Zheng Sichuan Agricultural University Triticeae Research Institute Dujiangyan City 611830 Sichuan China

Search for other papers by Y. Zheng in
Current site
Google Scholar
PubMed Close
Pages:
523–531
Online Publication Date:
05 Dec 2008
Publication Date:
01 Dec 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.36.2008.4.1
Keywords:
Glu-D1-1 ; HMW-GS; sequence analysis
Restricted access

A novel HMW-GS of Dx5** with slightly faster migration rate than that of Dx5, was found in a Tibet bread wheat landrace using SDS-PAGE. Moreover, Dx5** is the subunit with the fastest migration rate in Glu-Dx locus. The gene for this subunit was isolated and its sequence was obtained in the present study. This gene was very similar to Dx5 both in nucleotide and deduced amino acid sequence. At the nucleotide sequence level, Dx5** different from Dx5 by the deletion of a 27 bp fragment and two nucleotide replacements at position 353(G/C) and 692(C/G), respectively. At the amino acid sequence level, Dx5** different from Dx5 by the deletion of a hexaploid (LGQGQQ) and a tripeptide (GQQ) repetitive motif and two amino acid replacements at position 118(C/S) and 231(A/G), respectively. These results suggested that the Dx5** was a derivation of Dx5 and was formed by replication slippage. Moreover, the specific cysteine (C) located at the beginning of the repetitive domain of Dx5, which proved to be critical for the end-use quality of wheat flours, was replaced by serine (S) in Dx5**.

  • Anderson, O.D., Greene, F.C. 1989. The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor. Appl. Genet. 77 :689–700.

    Greene F.C. , 'The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat ' (1989 ) 77 Theor. Appl. Genet. : 689 -700 .

    • Search Google Scholar

  • Anderson, O.D., Greene, F.C., Yip, R.E., Halford, N.G., Shewry, P.R., Malpica-Romero, J.M. 1989. Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv. Cheyenne. Nucleic Acids Res. 17 :461–462.

    Malpica-Romero J.M. , 'Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv. Cheyenne ' (1989 ) 17 Nucleic Acids Res. : 461 -462 .

    • Search Google Scholar

  • D’Ovidio, R., Lafiandra, D., Porceddu, R. 1996. Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat. Theor. Appl. Genet. 93 :1048–1053.

    Porceddu R. , 'Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat ' (1996 ) 93 Theor. Appl. Genet. : 1048 -1053 .

    • Search Google Scholar

  • De Bustos, A., Jouve, N. 2003. Characterisation and analysis of new HMW-glutenin alleles encoded by the Glu-R1 locus of Secale cereale . Theor. Appl. Genet. 107 :74–83.

    Jouve N. , 'Characterisation and analysis of new HMW-glutenin alleles encoded by the Glu-R1 locus of Secale cereale ' (2003 ) 107 Theor. Appl. Genet. : 74 -83 .

    • Search Google Scholar

  • De Bustos, A., Rubio, P., Jouve, N. 2000. Molecular characterisation of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat. Theor. Appl. Genet. 100 :1085–1094.

    Jouve N. , 'Molecular characterisation of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat ' (2000 ) 100 Theor. Appl. Genet. : 1085 -1094 .

    • Search Google Scholar

  • Forde, J., Malpica, J.M., Halford, N.G., Shewry, P.R., Anderson, O.D., Greene F.C., Miflin, B.J. 1985. The nucleotide sequence of a HMW subunit gene located on chromosome 1A of wheat ( Triticum aestivum L.). Nucleic Acids Res. 13 :6817–6832.

    Miflin B.J. , 'The nucleotide sequence of a HMW subunit gene located on chromosome 1A of wheat (Triticum aestivum L.) ' (1985 ) 13 Nucleic Acids Res. : 6817 -6832 .

    • Search Google Scholar

  • Guo, Z.F., Yan, Z.H., Wang, J.R., Wei, Y.M., Zheng, Y.L. 2005. Characterization of HMW prolamines and their coding sequences from Crithopsis delileana . Hereditas 142 :56–64.

    Zheng Y.L. , 'Characterization of HMW prolamines and their coding sequences from Crithopsis delileana ' (2005 ) 142 Hereditas : 56 -64 .

    • Search Google Scholar

  • Halford, N.G., Field, J.M., Blair, H., Urwin, P., Moore, K., Robert, L., Thompson, R., Flavell, R.B., Tatham, A.S., Shewry, P.R. 1992. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat ( Triticum aestivum L.) indicates quantitative effects on grain quality. Theor. Appl. Genet. 83 :373–378.

    Shewry P.R. , 'Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality ' (1992 ) 83 Theor. Appl. Genet. : 373 -378 .

    • Search Google Scholar

  • Halford, N.G., Forde, J., Anderson, O.D., Greene, F.C., Shewry, P.R. 1987. The nucleotide and deduced amino-acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat ( Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor. Appl. Genet. 75 :117–126.

    Shewry P.R. , 'The nucleotide and deduced amino-acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D ' (1987 ) 75 Theor. Appl. Genet. : 117 -126 .

    • Search Google Scholar

  • Lafiandra, D., D’Ovidio, R., Porceddu, E., Margiotta, B., Colaprico, G. 1993. New data supporting high Mr glutenin subunit 5 as determinant of quality differences among the pairs 5 + 10 vs 2 + 12. J. Cereal Sci. 18 :197–205.

    Colaprico G. , 'New data supporting high Mr glutenin subunit 5 as determinant of quality differences among the pairs 5 + 10 vs 2 + 12 ' (1993 ) 18 J. Cereal Sci. : 197 -205 .

    • Search Google Scholar

  • Lawrence, G.J., Shepherd, K.W. 1981. Chromosomal location of genes controlling seed protein in species related to wheat, Theor. Appl. Genet. 59 :25–31.

    Shepherd K.W. , 'Chromosomal location of genes controlling seed protein in species related to wheat ' (1981 ) 59 Theor. Appl. Genet. : 25 -31 .

    • Search Google Scholar

  • Li, W., Wan, Y., Liu, Z., Liu, K., Liu, X., Li, B., Li, Z., Zhang, X., Dong, Y., Wang, D. 2004. Molecular characterization of HMW glutenin subunit allele 1B×14: further insights into the evolution of Glu-B1-1 alleles in wheat and related species. Theor. Appl. Genet. 109 :1093–1104.

    Wang D. , 'Molecular characterization of HMW glutenin subunit allele 1B×14: further insights into the evolution of Glu-B1-1 alleles in wheat and related species ' (2004 ) 109 Theor. Appl. Genet. : 1093 -1104 .

    • Search Google Scholar

  • Liu, Z., Yan, Z., Wan, Y., Liu, K., Zheng, Y., Wang, D. 2003. Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops speciecs. Theor. Appl. Genet. 106 : 1368–1378.

    Wang D. , 'Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops speciecs ' (2003 ) 106 Theor. Appl. Genet. : 1368 -1378 .

    • Search Google Scholar

  • Payne, P.I. 1987. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu. Rev. Plant Physiol. 38 :141–153.

    Payne P.I. , 'Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality ' (1987 ) 38 Annu. Rev. Plant Physiol. : 141 -153 .

    • Search Google Scholar

  • Payne, P.I., Lawrence, G.J. 1983. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 , and Glu-D1 which code for high molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11 :29–35.

    Lawrence G.J. , 'Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high molecular-weight subunits of glutenin in hexaploid wheat ' (1983 ) 11 Cereal Res. Comm. : 29 -35 .

    • Search Google Scholar

  • Reddy, P., Appels, R. 1993. Analysis of a genomic DNA segment carrying the wheat high-molecular-weight (HMW) glutenin Bx17 subunit and its use as an RFLP marker. Theor. Appl. Genet. 85 :616–624.

    Appels R. , 'Analysis of a genomic DNA segment carrying the wheat high-molecular-weight (HMW) glutenin Bx17 subunit and its use as an RFLP marker ' (1993 ) 85 Theor. Appl. Genet. : 616 -624 .

    • Search Google Scholar

  • Sambrook, J., Fritsch, E.F., Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press (2nd ed.), pp. 584–585.

  • Shewry, P.R., Halford, N.G., Tatham, A.S. 1989. The high-molecular-weight subunits of wheat, barely and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. Oxford Surveys Plant Mol. Cell Biol. 6 :163–219.

    Tatham A.S. , 'The high-molecular-weight subunits of wheat, barely and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality ' (1989 ) 6 Oxford Surveys Plant Mol. Cell Biol. : 163 -219 .

    • Search Google Scholar

  • Shewry, P.R., Halford, N.G. 2002. Cereal seed storage proteins: structures, properties and role in grain utilization. J. Exp. Bot. 53 :947–958.

    Halford N.G. , 'Cereal seed storage proteins: structures, properties and role in grain utilization ' (2002 ) 53 J. Exp. Bot. : 947 -958 .

    • Search Google Scholar

  • Shewry, P.R., Gilbert, S.M., Savage, A.W.J., Tatham, A.S., Wan, Y.F., Belton, P.S., Wellner, N., D’Ovidio, R., Békés, F., Halford, N.G. 2003. Sequence and properties of HMW subunit 1B×20 from pasta wheat (Triticum durum) which is associated with poor end use properties. Theor. Appl. Genet. 106 :744–750.

    Halford N.G. , 'Sequence and properties of HMW subunit 1B×20 from pasta wheat (Triticum durum) which is associated with poor end use properties ' (2003 ) 106 Theor. Appl. Genet. : 744 -750 .

    • Search Google Scholar

  • Shewry, P.R., Tatham, A.S., Barro P., Lazzeri, P. 1995. Biotechnology of breadmaking: unraveling and manipulating the multi-protein gluten complex. Biotechnology 13 :1185–1190.

    Lazzeri P. , 'Biotechnology of breadmaking: unraveling and manipulating the multi-protein gluten complex ' (1995 ) 13 Biotechnology : 1185 -1190 .

    • Search Google Scholar

  • Sugiyama, T., Rafalski, A., Peterson, D., Soll, D. 1985. A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res. 13 :8729–8737.

    Soll D. , 'A wheat HMW glutenin subunit gene reveals a highly repeated structure ' (1985 ) 13 Nucleic Acids Res. : 8729 -8737 .

    • Search Google Scholar

  • Thompson, R.D., Bartels, D., Harberd, N.P. 1985. Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res. 13 :6833–6846.

    Harberd N.P. , 'Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit ' (1985 ) 13 Nucleic Acids Res. : 6833 -6846 .

    • Search Google Scholar

  • Wan, Y.F., Yan, Z.H., Liu, K.F., Zheng, Y.L., Renato, D., Shewry, P.R., Halford, N.G., Wang, D.W. 2005. Comparative analysis of the D genome-encoded high-molecular weightsubunits of glutenin. Theor. Appl. Genet. 111 :1183–1190.

    Wang D.W. , 'Comparative analysis of the D genome-encoded high-molecular weightsubunits of glutenin ' (2005 ) 111 Theor. Appl. Genet. : 1183 -1190 .

    • Search Google Scholar

  • Wang, J.R., Yan, Z.H., Wei Y.M., Zheng, Y.L. 2004. A novel high molecular weight glutenin subunit gene Ee1.5 from Elytrigia elongata (Host) Nevski. J. Cereal Sci. 40 :289–294.

    Zheng Y.L. , 'A novel high molecular weight glutenin subunit gene Ee1.5 from Elytrigia elongata (Host) Nevski ' (2004 ) 40 J. Cereal Sci. : 289 -294 .

    • Search Google Scholar

  • Wang, J.R., Yan, Z.H., Wei, Y.M., Zheng, Y.L. 2006. Characterization of high-molecular-weight glutenin Elytrigia elongata . Plant Breeding 125 :89–95.

    Zheng Y.L. , 'Characterization of high-molecular-weight glutenin Elytrigia elongata ' (2006 ) 125 Plant Breeding : 89 -95 .

    • Search Google Scholar

  • Yan, Z.H., Dai, S.F., Liu, D.C., Wei, Y.M., Zheng, Y.L. 2007. Allelic variation of high molecular weight glutenin subunits in the hexaploid wheat landraces of Tibet. China. Int. J. Agri. Res. 2(9) :838–843.

    Zheng Y.L. , 'Allelic variation of high molecular weight glutenin subunits in the hexaploid wheat landraces of Tibet ' (2007 ) 2 China. Int. J. Agri. Res. : 838 -843 .

    • Search Google Scholar

  • Yan, Z.H., Wan, Y.F., Liu, K.F., Zheng, Y.L., Wang, D.W. 2002. Identification of a novel HMW gultenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Sci. Bull. 47 :220–225.

    Wang D.W. , 'Identification of a novel HMW gultenin subunit and comparison of its amino acid sequence with those of homologous subunits ' (2002 ) 47 Chinese Sci. Bull. : 220 -225 .

    • Search Google Scholar

  • Yan, Z.H., Wei, Y.M., Wang, J.R., Liu, D.C., Dai, S.F., Zheng, Y.L. 2006. Characterization of two HMW glutenin subunit genes from Taenitherum Nevski. Genetica 127 :267–276.

    Zheng Y.L. , 'Characterization of two HMW glutenin subunit genes from Taenitherum Nevski ' (2006 ) 127 Genetica : 267 -276 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

 

 

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Dec 2024 143 0 0
Jan 2025 80 0 0
Feb 2025 95 0 0
Mar 2025 82 0 0
Apr 2025 26 0 0
May 2025 0 0 0
Jun 2025 0 0 0