Cereal Research Communications
Volume/Issue:
Volume 36: Issue 4
Comparison of PCR-based DNA markers for using different Lr19 and Lr24 leaf rust resistance wheat sources
Authors:
A. Uhrin Agricultural Research Institute of the Hungarian Academy of Sciences H-2462 Martonvásár Hungary

Search for other papers by A. Uhrin in
Current site
Google Scholar
PubMed Close
,
L. Láng Agricultural Research Institute of the Hungarian Academy of Sciences H-2462 Martonvásár Hungary

Search for other papers by L. Láng in
Current site
Google Scholar
PubMed Close
, and
Z. Bedő Agricultural Research Institute of the Hungarian Academy of Sciences H-2462 Martonvásár Hungary

Search for other papers by Z. Bedő in
Current site
Google Scholar
PubMed Close
Pages:
533–541
Online Publication Date:
05 Dec 2008
Publication Date:
01 Dec 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.36.2008.4.2
Keywords:
SCAR marker; STS marker; leaf rust resistance; MAS
Restricted access

Both resistance genes Lr19 and Lr24 originate from Agropyron elongatum . The gene Lr24 is derived from two different translocations: 1BS/3Ag (‘Amigo’) or 3DS/3Ag (‘Agent’). The use of molecular markers makes selection easier during the breeding process as well as in the selection of the parents. In this study, two markers were used to identify the gene Lr19 (GbF/R 130 , SCS265 512 ) and four different markers (J9/1-2 310 , SC-H5 700 , SCS1302 613 and SCS1326 607 ) were available to search for the gene Lr24 . The GbF/ R130 marker for gene Lr19 worked well, but the SCAR marker SCS265 512 proved to be easier to use in MAS. SCAR markers SCS1302 613 and SCS1326 607 proved to be highly reliable and effective for gene Lr24 not only in Agent-derived sources but also in ‘Amigo’ derivatives. The STS marker J9/1-2 310 and the SCAR marker SC-H5 700 required several modifications and were effective only in ‘Agent’ offsprings.

  • Bai, D., Knott, D.R. 1992. Suppression of rust resistance in bread wheat (Triticum aestivum) by D-genome chromosomes. Genome 35 :276–282.

    Knott D.R. , 'Suppression of rust resistance in bread wheat (Triticum aestivum) by D-genome chromosomes ' (1992 ) 35 Genome : 276 -282 .

    • Search Google Scholar

  • Bhardwaj, S.C., Prashar, M., Kumar, S., Jain, S.K., Datta, D. 2005. Lr19 resistance in wheat become susceptible to Puccinia triticina in India. Plant Disease 89 :1360 DOI:10.1094/PD-89-1360A

    Datta D. , 'Lr19 resistance in wheat become susceptible to Puccinia triticina in India ' (2005 ) 89 Plant Disease : 1360 -.

    • Search Google Scholar

  • Dedryver, F., Jubier, M.F., Thouverin, J., Goyeau, H. 1996. Molecular markers linked to leaf rust resistance gene Lr24 in different wheat cultivars. Genome 39 :830–835.

    Goyeau H. , 'Molecular markers linked to leaf rust resistance gene Lr24 in different wheat cultivars ' (1996 ) 39 Genome : 830 -835 .

    • Search Google Scholar

  • Dyck, P.L. 1987. The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29 :467–469.

    Dyck P.L. , 'The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat ' (1987 ) 29 Genome : 467 -469 .

    • Search Google Scholar

  • Friebe, B., Jiang, J., Raupp, W.J., McIntosh, R.A., Gill, B.S. 1996. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91 :59–87.

    Gill B.S. , 'Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status ' (1996 ) 91 Euphytica : 59 -87 .

    • Search Google Scholar

  • Gál, M., Vida, G., Uhrin, A., Bedő, Z., Veisz, O. 2007. Incorporation of leaf rust genes into winter wheat genotypes using marker-assisted selection. Acta Agronomica Hungarica 55 :149–156.

    Veisz O. , 'Incorporation of leaf rust genes into winter wheat genotypes using marker-assisted selection ' (2007 ) 55 Acta Agronomica Hungarica : 149 -156 .

    • Search Google Scholar

  • Gupta, A.K., Saini, R.G., Gupta, S., Malhotra, S. 1984. Genetic analysis of two wheat cultivars, “Sonalika” and “WL711” for reaction to leaf rust (Puccinia recondita) . Theor. Appl. Genet. 67 :215–217.

    Malhotra S. , 'Genetic analysis of two wheat cultivars, “Sonalika” and “WL711” for reaction to leaf rust (Puccinia recondita) ' (1984 ) 67 Theor. Appl. Genet. : 215 -217 .

    • Search Google Scholar

  • Gupta, S.K., Charpe, A., Prabhu, K.V., Haque, Q.M.R. 2006a. Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat. Theor. Appl. Genet. 113 :1027–1036.

    Haque Q.M.R. , 'Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat ' (2006 ) 113 Theor. Appl. Genet. : 1027 -1036 .

    • Search Google Scholar

  • Gupta, S.K., Charpe, A., Prabhu, K.V., Haque, Q.M.R., Prabhu, K.V. 2006b. Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica 150 :233–240.

    Prabhu K.V. , 'Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat ' (2006 ) 150 Euphytica : 233 -240 .

    • Search Google Scholar

  • Huerta-Espino, J., Singh, R.P. 1994. First report of virulence to wheat leaf rust resistance gene Lr19 in Mexico. Plant Disease 78 :640–644.

    Singh R.P. , 'First report of virulence to wheat leaf rust resistance gene Lr19 in Mexico ' (1994 ) 78 Plant Disease : 640 -644 .

    • Search Google Scholar

  • Jiang, J.B., Friebe, B., Gill, B.S. 1994. Chromosome painting of Amigo wheat. Theor. Appl. Genet. 89 :811–813.

    Gill B.S. , 'Chromosome painting of Amigo wheat ' (1994 ) 89 Theor. Appl. Genet. : 811 -813 .

    • Search Google Scholar

  • Knott, D.R. 1968. Translocations involving Triticum chromosomes and Agropyron chromosomes carrying leaf rust resistance. Can. J. Genet. Cytol. 10 :695–696.

    Knott D.R. , 'Translocations involving Triticum chromosomes and Agropyron chromosomes carrying leaf rust resistance ' (1968 ) 10 Can. J. Genet. Cytol. : 695 -696 .

    • Search Google Scholar

  • Knott, D.R. 1980. Mutation of a gene for yellow pigment linked to Lr19 in wheat. Can. J. Genet. Cytol. 22 :651–654.

    Knott D.R. , 'Mutation of a gene for yellow pigment linked to Lr19 in wheat ' (1980 ) 22 Can. J. Genet. Cytol. : 651 -654 .

    • Search Google Scholar

  • Manninger, K. 2002. Effective resistance genes as sources of resistance against Hungarian wheat rusts. Czech J. Genet. Plant Breed. 38 :153–154.

    Manninger K. , 'Effective resistance genes as sources of resistance against Hungarian wheat rusts ' (2002 ) 38 Czech J. Genet. Plant Breed. : 153 -154 .

    • Search Google Scholar

  • Marais, G.F. 1992. The modification of a common wheat- Thinopyrum distichum translocated chromosome with a locus homeoallelic to Lr19 . Theor. Appl. Genet. 85 :73–78.

    Marais G.F. , 'The modification of a common wheat-Thinopyrum distichum translocated chromosome with a locus homeoallelic to Lr19 ' (1992 ) 85 Theor. Appl. Genet. : 73 -78 .

    • Search Google Scholar

  • Marais, G.F., Marais, A.S., Groenwald, J.Z. 2001. Evaulation and reduction of Lr19-149, a recombined form of the Lr19 translocation of wheat. Euphytica 121 :289–295.

    Groenwald J.Z. , 'Evaulation and reduction of Lr19-149, a recombined form of the Lr19 translocation of wheat ' (2001 ) 121 Euphytica : 289 -295 .

    • Search Google Scholar

  • Mesterházy, A., Bartos, P., Goyeau, H., Niks, R.E., Csősz, M., Andreson, O., Casulli, F., Ittu, M., Jones, E., Manisterski, J., Manninger, K., Pasquini, M., Rubiales, D., Schachermayr, G., Strzembicka, A., Szunics, L., Todorova, M., Unger, O., Vanco, B., Vida, G., Walther, U. 2000. European virulence survey for leaf rust in wheat. Agronomie 20 :793–804.

    Walther U. , 'European virulence survey for leaf rust in wheat ' (2000 ) 20 Agronomie : 793 -804 .

    • Search Google Scholar

  • Nocente, F., Gazza, L., Pasquini, M. 2006. Evaluation of leaf rust resistance genes Lr1, Lr9, Lr24, Lr47 and their introgression into wheat cultivars by marker-assisted selection. Euphytica. DOI 10.1007/s10681-006-9334-x

  • Prabhu, K.V., Gupta, S.K., Charpe, A., Koul, S. 2004. SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum -derived gene Lr24 in wheat: a revision. Plant Breeding 123 :417–420.

    Koul S. , 'SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum-derived gene Lr24 in wheat: a revision ' (2004 ) 123 Plant Breeding : 417 -420 .

    • Search Google Scholar

  • Prins, R., Groenewald, J.Z., Marais, G.F., Snape, J.W. 2001. AFLP and STS tagging of Lr19 , a gene conferring resistance to leaf rust in wheat. Theor. Appl. Genet. 103 :618–624.

    Snape J.W. , 'AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat ' (2001 ) 103 Theor. Appl. Genet. : 618 -624 .

    • Search Google Scholar

  • Schachermayr, G.M., Messmer, M.M., Feuillet, C., Winzeler, H., Winzeler, M., Keller, B. 1995. Identification of molecular markers linked to the Agropyron elongatum -derived leaf rust resistance gene Lr24 in wheat. Theor. Appl. Genet. 90 :982–990.

    Keller B. , 'Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat ' (1995 ) 90 Theor. Appl. Genet. : 982 -990 .

    • Search Google Scholar

  • Schnurbusch, T., Paiilard, S., Schori, A., Messmer, M., Schachermayr, G., Winzeler, M., Keller, B. 2004. Dissection of quantitive and durable laef rust resistance in Swiss winter wheat reveals amajor resistance QTL in the Lr34 chromosomal region. Theor. Appl. Genet. 108 :477–484.

    Keller B. , 'Dissection of quantitive and durable laef rust resistance in Swiss winter wheat reveals amajor resistance QTL in the Lr34 chromosomal region ' (2004 ) 108 Theor. Appl. Genet. : 477 -484 .

    • Search Google Scholar

  • Sears, E.R. 1977. Analysis of wheat- Agropyron recombinant chromosomes. In: Sanchez-Monge, E., Garcia-Olmedo, F. (eds), Interspecific hybridization in plant breeding. Proceedings of the 8th EUCARPIA Congress, Madrid, Spain, 1977. pp. 63–72.

  • Sharma, D., Knott, D.R. 1966. The transfer of leaf-rust resistance from Agropyron to Triticum by irradiation. Can. J. Genet. Cytol. 8 :137–143.

    Knott D.R. , 'The transfer of leaf-rust resistance from Agropyron to Triticum by irradiation ' (1966 ) 8 Can. J. Genet. Cytol. : 137 -143 .

    • Search Google Scholar

  • Smith, E.R., Schlehuber, A.M., Young, H.C., Edwards, L.H. 1968. Registration of Agent Wheat. Crop Science 8 :511–512.

    Edwards L.H. , 'Registration of Agent Wheat ' (1968 ) 8 Crop Science : 511 -512 .

  • William, M., Singh, R.P., Huera-Espino, J., Islas, S.O., Hoisington, D. 2003. Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93 :153–159.

    Hoisington D. , 'Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat ' (2003 ) 93 Phytopathology : 153 -159 .

    • Search Google Scholar

  • Zhang, W., Soria, M.A., Goyal, S., Dubcovsky, J., Lukaszewski, A.J., Kolmer, J. 2005. Molecular characterization of durum and common wheat recombinant lines carrying leaf rust resistance (Lr19) and yellow pigment (Y) genes from Lophopyrum ponticum . Theor. Appl. Genet. 111 :573–582.

    Kolmer J. , 'Molecular characterization of durum and common wheat recombinant lines carrying leaf rust resistance (Lr19) and yellow pigment (Y) genes from Lophopyrum ponticum ' (2005 ) 111 Theor. Appl. Genet. : 573 -582 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

 

 

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Oct 2024 68 0 0
Nov 2024 91 0 0
Dec 2024 110 1 1
Jan 2025 78 0 0
Feb 2025 71 1 1
Mar 2025 66 0 0
Apr 2025 0 0 0