Cereal Research Communications
Volume/Issue:
Volume 36: Issue 4
Identification of wheat line YW243 on comprehensive resistance to several diseases by pathogens and molecular markers
Authors:
H. Xie

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Z. Lin Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Beijing 100081 China

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Z. Zhang Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Beijing 100081 China

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L. Du Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Beijing 100081 China

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Z. Xin Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Beijing 100081 China

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Y. Ma Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Beijing 100081 China

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X. Ye Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Beijing 100081 China

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X. Chen Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Beijing 100081 China

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Pages:
543–552
Online Publication Date:
05 Dec 2008
Publication Date:
01 Dec 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.36.2008.4.3
Keywords:
Triticum aestivum ; barley yellow dwarf virus; powdery mildew; stripe rust; molecular markers; pyramid breeding
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The common wheat line, YW243, developed in our research group, was tested for the resistances of barley yellow dwarf virus (BYDV), powdery mildew (Pm) and stripe rust in field, and was analyzed by molecular markers for convenient trace of the resistant genes in breeding. Genomic in situ hybridization (GISH) analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay further demonstrated that YW243 was a homozygous multiple translocation line of Triticum aestivum, Thinopyrum intermedium and Secale cereale (T7DS·7DL-7XL & 1BL·1RS). The disease resistance test and marker analysis showed that YW243 carried seven resistance genes to the three diseases, including Bdv2 to BYDV on 7DL-7XL, Pm4 to powdery mildew on 2AL, Yr2, Yr9, Sr 31 and Lr26 and a new Yr to stripe rust on 7B, 1BL, 1RS and 2BL. Restriction fragment length polymorphism (RFLP) markers Xpsr687 and Xwg380 , sequence tagged site (STS) marker STS 1700 , simple sequence repeat (SSR) markers Xgwmc364 and Xgwm582 , SSR markers Xgwm388 and Xgwm501 can be used as diagnostic tools to track Bdv2, Pm4, Yr2, Yr9 and Yr in YW243 , respectively; and two amplified fragment length polymorphism (AFLP) markers M54E63 - 700 and M54E64 - 699 can also be used to select Yr in YW243 .

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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