Cereal Research Communications
Volume/Issue:
Volume 36: Issue 4
PCR identification of Fusarium graminearum isolated from wheat grain
Authors:
Zs. Koncz Eszterházy Károly College Laboratory of Molecular Biology H-3300 Eger Hungary

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K. Huszti Eszterházy Károly College Laboratory of Molecular Biology H-3300 Eger Hungary

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Z. Naár Eszterházy Károly College Laboratory of Molecular Biology H-3300 Eger Hungary

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A. Kiss Eszterházy Károly College Laboratory of Molecular Biology H-3300 Eger Hungary

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Á. Szécsi Hungarian Academy of Sciences Plant Protection Institute P.O. Box 102 H-1525 Budapest Hungary

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Pages:
623–630
Online Publication Date:
05 Dec 2008
Publication Date:
01 Dec 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.36.2008.4.11
Keywords:
Fusarium graminearum ; PCR; species-specific primers; chip electrophoresis; wheat grain
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Species-specific PCR assay was used for the identification of Hungarian Fusarium graminearum isolates in pure mycelial culture. The Fg16F/Fg16R primer pair of the three known species-specific primers appeared to be the most appropriate one to identify F. graminearum .Two methods were used for comparative determination of the amplicon size of F. graminearum strains: traditional agarose gel electrophoresis, and chip electrophoresis. Our results have shown that the chip electrophoresis is an easy-to-use, time-efficient substitute for conventional agarose gel electrophoresis; moreover it provides a more precise size determination of amplicons. Amplicon size ranging from 415 bp to 421 bp in tested isolates may be associated with genetic diversity in the Hungarian population of F. graminearum .The PCR assay described in this study can be used for the routine detection and identification of F. graminearum without isolation and morphological investigation of this fungus.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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