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  • 1 USDA-ARS Crop Production and Pest Control Unit 915 West State Street West Lafayette IN 47907 USA
  • | 2 USDA-ARS Plant Stress and Germplasm Development Unit 3810 4th Street Lubbock TX 79415 USA
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Virus-induced gene silencing (VIGS) is an important tool for rapid assessment of gene function in plants. The ability of the Barley stripe mosaic virus (BSMV) VIGS system to simultaneously silence two genes was assessed by comparing the extent of down-regulation of the wheat PDS and SGT1 genes after wheat plants were infected with BSMV-VIGS constructs carrying 178bp fragments of the PDS and SGT1 genes linked in cis or after infection with single-gene silencing BSMV constructs carrying PDS or SGT1 alone or with a mixture of the single-gene constructs. Although significant variation occurred in these studies it was clearly demonstrated that two wheat genes can be silenced simultaneously by a single BSMV-VIGS construct.

  • Azevedo, C., Sadanandom, A., Kitagawa, K., Freialdenhoven, A., Shirasu, K., Schulze-Lefert, P. 2002. The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance. Science 295 :2073–2076.

    Schulze-Lefert P. , 'The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance ' (2002 ) 295 Science : 2073 -2076.

    • Search Google Scholar
  • Baulcombe, D.C. 1999. Fast forward genetics based on virus-induced gene silencing. Curr Opin Plant Biol. 2 :109–113.

    Baulcombe D.C. , 'Fast forward genetics based on virus-induced gene silencing ' (1999 ) 2 Curr Opin Plant Biol. : 109 -113.

    • Search Google Scholar
  • Burch-Smith, T.M., Anderson, J.C., Martin, G.B., Dinesh-Kumar, S.P. 2004. Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J. 39 :734–746.

    Dinesh-Kumar S.P. , 'Applications and advantages of virus-induced gene silencing for gene function studies in plants ' (2004 ) 39 Plant J. : 734 -746.

    • Search Google Scholar
  • Petty, I.T., Hunter, B.G., Wei, N., Jackson, A.O. 1989. Infectious barley stripe mosaic virus RNA transcribed in vitro from full-length genomic cDNA clones. Virology 171 :342–349.

    Jackson A.O. , 'Infectious barley stripe mosaic virus RNA transcribed in vitro from full-length genomic cDNA clones ' (1989 ) 171 Virology : 342 -349.

    • Search Google Scholar
  • Pogue, G.P., Lindbo, J.A., Garger, S.J., Fitzmaurice, W.P. 2002. Making an ally from an enemy: plant virology and the new agriculture. Annu. Rev. Phytopathol. 40 :45–74.

    Fitzmaurice W.P. , 'Making an ally from an enemy: plant virology and the new agriculture ' (2002 ) 40 Annu. Rev. Phytopathol. : 45 -74.

    • Search Google Scholar
  • Scofield, S.R., Huang, L., Brandt, A.S., Gill, B.S. 2005. Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway. Plant Physiol. 138 :2165–2173.

    Gill B.S. , 'Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway ' (2005 ) 138 Plant Physiol. : 2165 -2173.

    • Search Google Scholar

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Cereal Research Communications
Language English
Size B5
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
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Springer Nature Switzerland AG
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ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)