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  • 1 Nanjing Agricultural University The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre & National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Jiangsu 210095 China
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Wangshuibai is an indigenous scab resistance germplasm originated from Jiangsu, China. To characterize the genetic basis of scab resistance in this germplasm, QTLs for type I and type II resistances were detected using a recombinant inbred line (RIL) population created by single seed descent from Nanda2419 × Wangshuibai and a molecular marker map of more than 4000 cM constructed using RAPD, SSR and STS markers. The major QTLs for type I resistance in Wangshuibai were mapped to chromosomes 4BL and 5AS, and those for type II resistance were mapped to chromosomes 3BS. In addition, a QTL on chromosome 2B showed association with both types of resistance. These QTLs were verified with QTL nearisogenic lines. We found, by mapping QTLs for agronomical traits in the same population, that on chromosomes 4BL and 5AS the scab resistance QTLs co-located with QTLs for plant height, thousand grain weight or flag leaf width. However, these associations could be break down by recombinant selection. We concluded that Wangshuibai is a valuable scab resistance gene resources and marker assisted selection would be of great help for its better utilization.

  • Anderson, J.A., Stack, R.W., Liu, S., Waldron, B.L., Field, A.D., Coyne, C., Moreno-Sevilla, B., Mitchell Fetch, J., Song, Q.J., Cregan, P.B., Frohberg, R.C. 2001. DNA markers for Fusarium head blight resistance QTL in two wheat populations. Theor. Appl. Genet. 102 :1164–1168.

    Frohberg R.C. , 'DNA markers for Fusarium head blight resistance QTL in two wheat populations ' (2001 ) 102 Theor. Appl. Genet. : 1164 -1168.

    • Search Google Scholar
  • Buerstmayr, H., Steiner, B., Lemmens, M., Ruckenbauer, P. 2000. Resistance to Fusarium head blight in winter wheat: heritability and trait associations. Crop Sci. 40 :1012–1018.

    Ruckenbauer P. , 'Resistance to Fusarium head blight in winter wheat: heritability and trait associations ' (2000 ) 40 Crop Sci. : 1012 -1018.

    • Search Google Scholar
  • Buerstmayr, H., Lemmens, M., Hartl, L., Doldi, L., Steiner, B., Stierschneider, M., Ruckenbauer, P. 2002. Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). Theor. Appl. Genet. 104 :84–91.

    Ruckenbauer P. , 'Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance) ' (2002 ) 104 Theor. Appl. Genet. : 84 -91.

    • Search Google Scholar
  • Buerstmayr, H., Steiner, B., Hartl, L., Griesser, M., Angerer, N., Lengauer, D., Miedaner, T., Schneider, B., Lemmens, M. 2003. Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor. Appl. Genet. 107 :503–508.

    Lemmens M. , 'Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread ' (2003 ) 107 Theor. Appl. Genet. : 503 -508.

    • Search Google Scholar
  • Chen, J., Griffey, C.A., Maroof, M.A.S., Stromberg, E.L., Biyashev, R.M., Zhao, W., Chappell, M.R., Pridgen, T.H., Dong, Y., Zeng, Z. 2006. Validation of two major quantitative trait loci for Fusarium head blight resistance in Chinese wheat line W14. Plant Breed. 125: 99–101.

    Zeng Z. , 'Validation of two major quantitative trait loci for Fusarium head blight resistance in Chinese wheat line W14 ' (2006 ) 125 Plant Breed. : 99 -101.

    • Search Google Scholar
  • Gervais, L., Dedryver, F., Morlais, J.Y., Bodusseau, V., Negre, S., Bilous, M., Groos, C., Trottet, M. 2003. Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat. Theor. Appl. Genet. 106 :961–970.

    Trottet M. , 'Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat ' (2003 ) 106 Theor. Appl. Genet. : 961 -970.

    • Search Google Scholar
  • Gilbert, J., Tekauz, A. 2000. Review: recent developments in research on Fusarium head blight of wheat in Canada. Can. J. Plant Pathol. 22: 1–8.

    Tekauz A. , 'Review: recent developments in research on Fusarium head blight of wheat in Canada ' (2000 ) 22 Can. J. Plant Pathol. : 1 -8.

    • Search Google Scholar
  • Jiang, G.L., Shi, J., Ward, R.W. 2007. QTL analysis of resistance to Fusarium head blight in the novel wheat germplasm CJ9306. I. Resistance to fungal spread. Theor. Appl. Genet. 116 :3–13.

    Ward R.W. , 'QTL analysis of resistance to Fusarium head blight in the novel wheat germplasm CJ9306. I. Resistance to fungal spread ' (2007 ) 116 Theor. Appl. Genet. : 3 -13.

    • Search Google Scholar
  • Klahr, A., Zimmermann, G., Wenzel, G., Mohler, V. 2007. Effects of environment, disease progress, plant height and heading date on the detection of QTLs for resistance to Fusarium head blight in an European winter wheat cross. Euphytica 154: 17–28.

    Mohler V. , 'Effects of environment, disease progress, plant height and heading date on the detection of QTLs for resistance to Fusarium head blight in an European winter wheat cross ' (2007 ) 154 Euphytica : 17 -28.

    • Search Google Scholar
  • Lin, F., Kong, Z.X., Zhu, H.L., Xue, S.L., Wu, J.Z., Tian, D.G., Wei, J.B., Zhang, C.Q., Ma, Z.Q. 2004. Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419 × Wangshuibai population. I. Type II resistance. Theor. Appl. Genet. 109: 1504–1511.

    Ma Z.Q. , 'Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419 × Wangshuibai population. I. Type II resistance ' (2004 ) 109 Theor. Appl. Genet. : 1504 -1511.

    • Search Google Scholar
  • Lin, F., Xue, S.L., Tian, D.G., Li, C.J., Cao, Y., Zhang, Z.Z., Zhang, C.Q., Ma, Z.Q. 2008. Mapping chromosomal regions affecting flowering time in a spring wheat RIL population. Euphytica DOI 10.1007/s10681-008-9724-3.

  • Lin, F., Xue, S.L., Zhang, Z.Z., Zhang, C.Q., Kong, Z.X., Yao, G.Q., Tian, D.G., Zhu, H.L., Li, C.J., Cao, Y., Wei, J.B., Luo, Q.Y., Ma, Z.Q. 2006. Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419 × Wangshuibai population. II: Type I resistance. Theor. Appl. Genet. 112: 528–535.

    Ma Z.Q. , 'Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419 × Wangshuibai population. II: Type I resistance ' (2006 ) 112 Theor. Appl. Genet. : 528 -535.

    • Search Google Scholar
  • Liu, S., Abate, Z.A., Lu, H., Musket, T., Davis, G.L., McKendry, A.L. 2007. QTL associated with Fusarium head blight resistance in the soft red winter wheat Ernie. Theor. Appl. Genet. 115 : 417–427.

    McKendry A.L. , 'QTL associated with Fusarium head blight resistance in the soft red winter wheat Ernie ' (2007 ) 115 Theor. Appl. Genet. : 417 -427.

    • Search Google Scholar
  • Ma, H.X., Zhang, K.M., Gao, L., Bai, G.H., Chen, H.G., Cai, Z.X., Lu, W.Z. 2006. Quantitative trait loci for resistance to Fusarium head blight and deoxynivalenol accumulation in Wangshuibai wheat under field conditions. Plant Pathol. 55 :739–745.

    Lu W.Z. , 'Quantitative trait loci for resistance to Fusarium head blight and deoxynivalenol accumulation in Wangshuibai wheat under field conditions ' (2006 ) 55 Plant Pathol. : 739 -745.

    • Search Google Scholar
  • Mesterhazy, A. 1995. Types and components of resistance to Fusarium head blight of wheat. Plant Breed. 114 :377–386.

    Mesterhazy A. , 'Types and components of resistance to Fusarium head blight of wheat ' (1995 ) 114 Plant Breed. : 377 -386.

    • Search Google Scholar
  • Paillard, S., Schnurbusch, T., Tiwari, R., Messmer, M., Winzeler, M., Keller, B., Schachermayr, G. 2004. QTL analysis of resistance to Fusarium head blight in Swiss winter wheat ( Triticum aestivum L.). Theor. Appl. Genet. 109 : 323–332.

    Schachermayr G. , 'QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.) ' (2004 ) 109 Theor. Appl. Genet. : 323 -332.

    • Search Google Scholar
  • Schroeder, H.W., Christensen, J.J. 1963. Factors affecting resistance of wheat to scab caused by Gibberella zeae . Phytopath. 53 :831–838.

    Christensen J.J. , 'Factors affecting resistance of wheat to scab caused by Gibberella zeae ' (1963 ) 53 Phytopath. : 831 -838.

    • Search Google Scholar
  • Shen, X., Ittu, M., Ohm, H. 2003. Quantitative trait loci conditioning resistance to Fusarium head blight in wheat line F201R. Crop Sci. 43 :850–857.

    Ohm H. , 'Quantitative trait loci conditioning resistance to Fusarium head blight in wheat line F201R ' (2003 ) 43 Crop Sci. : 850 -857.

    • Search Google Scholar
  • Somers, D.J., Fedak, G., Savard, M. 2003. Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46 :555–564.

    Savard M. , 'Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat ' (2003 ) 46 Genome : 555 -564.

    • Search Google Scholar
  • Steiner, B., Lemmens, M., Griesser, M., Scholz, U., Schondelmaier, J., Buerstmayr, H. 2004. Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor. Appl. Genet. 109 : 215–224.

    Buerstmayr H. , 'Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana ' (2004 ) 109 Theor. Appl. Genet. : 215 -224.

    • Search Google Scholar
  • Xue, S.L., Zhang, Z.Z., Lin, F., Kong, Z.X., Cao, Y., Li, C.J., Yi, H.Y., Mei, M.F., Zhao, D.M., Zhu, H.L., Xu, H.B., Wu, J.Z., Tian, D.G., Zhang, C.Q., Ma, Z.Q. 2008. A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor. Appl. Genet. 117 :181–189.

    Ma Z.Q. , 'A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags ' (2008 ) 117 Theor. Appl. Genet. : 181 -189.

    • Search Google Scholar
  • Yang, Z., Gilbert, J., Fedak, G., Somers, D.J. 2005. Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled haploid spring wheat population. Genome 48 :187–196.

    Somers D.J. , 'Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled haploid spring wheat population ' (2005 ) 48 Genome : 187 -196.

    • Search Google Scholar
  • Zhou, W.C., Kolb, F.L., Bai, G.H., Shaner, G., Domier, L.L. 2002. Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers. Genome 45:719–727.

    Domier L.L. , 'Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers ' (2002 ) 45 Genome : 719 -727.

    • Search Google Scholar