Authors:
Marie DufresneUniversité Paris-Sud Institut de Génétique et Microbiologie 91405 Orsay Cedex France

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Theo LeePlant Research International B.V. P.O. Box 16 6500 AA Wageningen The Netherlands

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Sarrah M’Barek

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X. Xu

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X. Zhang

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Taiguo Liu

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Wenwei Zhang

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Gert KemaPlant Research International B.V. P.O. Box 16 6500 AA Wageningen The Netherlands

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Marie-Josée DaboussiUniversité Paris-Sud Institut de Génétique et Microbiologie 91405 Orsay Cedex France

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Cees WaalwijkPlant Research International B.V. P.O. Box 16 6500 AA Wageningen The Netherlands

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Transposon mutagenesis was applied to generate mutants in Fusarium graminearum . The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum , making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome.

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  • Mehrabi, R., van der Lee, T.A.J., Waalwijk, C., Kema, G.H.J. 2006, MgSlt2 , a cellular integrity MAP kinase gene of the fungal wheat pathogen Mycosphaerella graminicola , is dispensable for penetration but essential for invasive growth. Molec. Plant-Microbe Interact. 19 :389–398.

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)