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  • 1 Université Paris-Sud Institut de Génétique et Microbiologie 91405 Orsay Cedex France
  • 2 Plant Research International B.V. P.O. Box 16 6500 AA Wageningen The Netherlands
  • 3 Liaoning Academy of Agricultural Sciences Plant Protection Institute Shenyang 110161 China
  • 4 Jiangsu Academy of Agricultural Sciences Institute of Genetics Zhongling Street 50 Nanjing Jiangsu 210014 China
  • 5 Chinese Academy of Agriculture Sciences State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Beijing 100094 China
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Transposon mutagenesis was applied to generate mutants in Fusarium graminearum . The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum , making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome.

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