Authors:
Doris LucyshynBOKU - University of Natural Resources and Applied Life Sciences Institute of Applied Genetics and Cell Biology Vienna Austria

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Shamsozoha AbolmaaliBOKU - University of Natural Resources and Applied Life Sciences Institute of Applied Genetics and Cell Biology Vienna Austria

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Hanna WeindorferBOKU - University of Natural Resources and Applied Life Sciences Institute of Applied Genetics and Cell Biology Vienna Austria

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Mehrdad ShamsBOKU - University of Natural Resources and Applied Life Sciences Institute of Applied Genetics and Cell Biology Vienna Austria

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Gerlinde WiesenbergerBOKU - University of Natural Resources and Applied Life Sciences Institute of Applied Genetics and Cell Biology Vienna Austria

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Eva WilhelmARC Seibersdorf Research GmbH Seibersdorf Austria

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Marc LemmensInstitute of Plant Production Biotechnology Department for Agrobiotechnology, IFA-Tulln, BOKU Vienna Vienna Austria

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Gerhard AdamBOKU - University of Natural Resources and Applied Life Sciences Institute of Applied Genetics and Cell Biology Vienna Austria

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The production of the trichothecene mycotoxin deoxynivalenol (DON) is an important virulence factor of the plant pathogenic fungus Fusarium graminearum on wheat. We have engineered a DON sensitive yeast strain and constructed a cDNA library from DON treated wheat suspension culture cells in a yeast expression vector. The library was used to select DON resistance conferring clones. Besides ORFs of unknown function, we found 3 classes of cDNAs that in addition to DON resistance conferred hypersensitivity to hygromycin and canavanine. The predicted functions of several of the wheat cDNAs (putative E3 ligase, ubiquitin specific protease, proteasome subunit) suggested a role for ubiquitin-proteasome mediated protein degradation in DON resistance. Results with a coupled wheat germ in vitro translation system and a GUS-luciferase fusion gene showed that DON is a powerful translation elongation inhibitor. The truncated proteins formed in the presence of DON most likely lead to ubiquitin depletion and consequently growth inhibition in yeast. Ubiquitin is essential for many processes in plants, including plant defense. Our results warrant the re-evaluation of the relevance of proteasome system components found to be differentially regulated during Fusarium infection.

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    Adam G. , 'Engineered bakers yeast as a sensitive bioassay indicator organism for the trichothecene toxin deoxynivalenol ' (2008 ) 72 J. Microbiol. Meth. : 306 -312 .

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    Ruckenbauer P. , 'The ability to detoxify the mycotoxin deoxynivalenol colocalizes with a major quantitative trait locus for Fusarium head blight resistance in wheat ' (2005 ) 18 Mol Plant Microbe Interact. : 1318 -1324 .

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    Adam G. , 'Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum ' (2007 ) 277 Mol. Genet. Genomics : 507 -17 .

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  • Mitterbauer, R., Adam, G. 2002. Saccharomyces cerevisae and Arabidopsis thaliana : useful model systems for the identification of molecular mechanisms involved in resistance of plants to toxins. Eur. J. Plant Pathol. 108 :699–703.

    Adam G. , 'Saccharomyces cerevisae and Arabidopsis thaliana: useful model systems for the identification of molecular mechanisms involved in resistance of plants to toxins ' (2002 ) 108 Eur. J. Plant Pathol. : 699 -703 .

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  • Mitterbauer, R., Poppenberger, B., Raditschnig, A., Lucyshyn, D., Lemmens, M., Glössl, J., Adam, G. 2004. Toxin-dependent utilization of engineered ribosomal protein L3 limits trichothecene resistance in transgenic plants. Plant Biotechnol. J. 2: 329–340.

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  • Poppenberger, B., Berthiller, F., Lucyshyn, D., Sieberer, T., Schuhmacher, R., Krska, R., Kuchler, K., Glössl, J., Luschnig, C., Adam, G. 2003. Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana . J. Biol. Chem. 278: 47905–47914.

    Adam G. , 'Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana ' (2003 ) 278 J. Biol. Chem. : 47905 -47914 .

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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