Cereal Research Communications
Volume/Issue:
Volume 36: Issue Supplement-6
Synthesis of an FHB-resistant durum disomic alien addition line with a pair of diploid wheatgrass chromosomes
Author: Prem Jauhar 1
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  • 1 Northern Crop Science Laboratory United States Department of Agriculture-Agricultural Research Service Fargo North Dakota 58105 USA
DOI:
https://doi.org/10.1556/crc.36.2008.suppl.b.13
Page Count:
77–82
Publication Date:
01 Sep 2008
Online Publication Date:
12 Sep 2008
Article Category:
Research Article
Restricted access

Durum wheat ( Triticum turgidum L., 2 n = 4 x = 28; AABB genomes) is an important cereal crop widely used for human consumption. Fusarium head blight (FHB), or scab, caused by the fungus Fusarium graminearum Schwabe, is a serious disease of durum wheat. Current durum cultivars have very little or no FHB resistance. A wild relative, diploid wheatgrass Lophopyrum elongatum (Host) Á. Löve (2 n = 2 x = 14; EE genome) is an excellent source of resistance. By crossing durum wheat with L. elongatum we produced F 1 hybrids that were male sterile. By backcrossing the F1 hybrids to the durum parent, followed by selfing, we obtained several fertile hybrid derivatives with FHB resistance. We isolated a disomic addition line (2 n = 28 + 2) with a pair of L. elongatum chromosomes. This alien addition line is meiotically regular and hence reproductively stable. The addition line is FHB-resistant with a mean infection of 6.5%, while the parental durum has 80% infection. Using various biochemical and molecular techniques — fluorescent genomic in situ hybridization (fl-GISH) and chromosome-specific markers — we have shown that the extra chromosome involved is 1E of L. elongatum . This is the first time that FHB resistance has been discovered on chromosome 1E.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
chromosome pairing; chromosome-specific markers; fluorescent genomic in situ hybridization (fl-GISH); Lophopyrum elongatum

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