Cereal Research Communications
Volume/Issue:
Volume 37: Issue 1
Transgenic wheat plants derived from Agrobacterium -mediated transformation of mature embryo tissues
Authors:
Y. Wang Chinese Academy of Agricultural Sciences National Key Facility of Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Science Beijing 100081 China

Search for other papers by Y. Wang in
Current site
Google Scholar
PubMed Close
,
M. Xu Chinese Academy of Sciences The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology Beijing 100101 China

Search for other papers by M. Xu in
Current site
Google Scholar
PubMed Close
,
G. Yin Chinese Academy of Agricultural Sciences National Key Facility of Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Science Beijing 100081 China

Search for other papers by G. Yin in
Current site
Google Scholar
PubMed Close
,
L. Tao Chinese Academy of Agricultural Sciences National Key Facility of Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Science Beijing 100081 China

Search for other papers by L. Tao in
Current site
Google Scholar
PubMed Close
,
D. Wang Chinese Academy of Sciences The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology Beijing 100101 China

Search for other papers by D. Wang in
Current site
Google Scholar
PubMed Close
, and
X. Ye Chinese Academy of Agricultural Sciences National Key Facility of Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Science Beijing 100081 China

Search for other papers by X. Ye in
Current site
Google Scholar
PubMed Close
Pages:
1–12
Online Publication Date:
05 Mar 2009
Publication Date:
01 Mar 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.37.2009.1.1
Keywords:
Agrobacterium tumefaciens ; genetic transformation; mature embryo; wheat; transgenic plant
Restricted access

Although significant progress has been made on Agrobacterium -mediated wheat transformation, the current methodologies use immature embryos as recipient tissues, a process which is labor intensive, time consuming and expensive. In this study, we have managed to develop an Agrobacterium -based transformation scheme using explants derived from mature embryos. Based on transient expression of β -glucuronidase (GUS) marker, mature embryo halves prepared from freshly imbibed seeds were generally most susceptible to Agrobacterium -mediated T-DNA transfer. According to the results of callus induction and shoot production, Yumai 66 and Lunxuan 208 showed higher selection and regeneration efficiency than Bobwhite. In line with this finding, fertile T 0 transgenic plants were most readily obtained for both spring and winter wheat when mature embryo halves were used for co-inoculation by Agrobacterium cells. The presence of the antibiotic selection marker ( nptII , encoding neomycin phosphotransferase II) in the T 0 plants was revealed by both genomic PCR amplification and the enzyme-linked immunosorbent assay (ELISA). Additional analysis showed that the transgene was stably inherited from the two different generations and segregated normally among the T 1 progenies. Further development along this line will raise the efficiency of wheat transformation and increase the use of this approach in the molecular breeding of wheat crop.

  • Cheng, M., Fry, J.E., Pang, S., Zhou, H., Hironaka, C.M., Duncan, D.R., Conner, T.W., Wan, Y. 1997. Genetic transformation of wheat mediated by Agrobacterium tumefaciens . Plant Physiol. 115 :971–980.

    Wan Y. , 'Genetic transformation of wheat mediated by Agrobacterium tumefaciens ' (1997 ) 115 Plant Physiol. : 971 -980 .

    • Search Google Scholar

  • Cheng, M., Hu, T., Layton, J., Liu, C.N., Fry, J.E. 2003. Desiccation of plant tissues post- Agrobacterium infection enhances T-DNA delivery and increases stable transformation efficiency in wheat. In Vitro Cell. Dev. Biol. Plant 39 :595–604.

    Fry J.E. , 'Desiccation of plant tissues post-Agrobacterium infection enhances T-DNA delivery and increases stable transformation efficiency in wheat ' (2003 ) 39 Vitro Cell. Dev. Biol. Plant : 595 -604 .

    • Search Google Scholar

  • Cheng, M., Lowe, B.A., Spencer, T.M., Ye, X., Armstrong, C.L. 2004. Factors influencing Agrobacterium -mediated transformation of monocotyledonous species. In Vitro Cell. Dev. Biol. Plant 40 :31–45.

    Armstrong C.L. , 'Factors influencing Agrobacterium-mediated transformation of monocotyledonous species ' (2004 ) 40 Vitro Cell. Dev. Biol. Plant : 31 -45 .

    • Search Google Scholar

  • Deblaere, R., Bytebier, B., de Greve, H., Deboeck, F., Schell, J., van Montagu, M., Leemans, J. 1985. Efficient octopine Ti plasmid derived vectors for Agrobacterium -mediated gene transfer to plants. Nucl. Acids Res. 13 :4777–4788.

    Leemans J. , 'Efficient octopine Ti plasmid derived vectors for Agrobacterium-mediated gene transfer to plants ' (1985 ) 13 Nucl. Acids Res. : 4777 -4788 .

    • Search Google Scholar

  • Dellaporta, S.L., Wood, J., Hicks, J.B. 1983. A plant DNA mini-preparation: Version II. Plant Mol. Biol. Rep. 1 :19–21.

    Hicks J.B. , 'A plant DNA mini-preparation: Version II ' (1983 ) 1 Plant Mol. Biol. Rep. : 19 -21 .

    • Search Google Scholar

  • Ditta, G., Stanfield, S., Corbin, D., Helinsnki, D.R. 1980. Broad host range cloning system for gram negative bacteria: Construction of gene bank of Rhizobium meliloti . Proc. Natl. Acad. Sci. USA 77 :7374–7351.

    Helinsnki D.R. , 'Broad host range cloning system for gram negative bacteria: Construction of gene bank of Rhizobium meliloti ' (1980 ) 77 Proc. Natl. Acad. Sci. USA : 7374 -7351 .

    • Search Google Scholar

  • Hu, T., Metz, S., Chay, C., Zhou, H.P., Biest, N., Chen, G., Cheng, M., Feng, X., Radionenko, M., Lu, F., Fry, J.E. 2003. Agrobacterium -mediated large-scale transformation of wheat ( Triticum aestivum L.) using glyphosate selection. Plant Cell Rep. 21 :1010–1019.

    Fry J.E. , 'Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection ' (2003 ) 21 Plant Cell Rep. : 1010 -1019 .

    • Search Google Scholar

  • Ignacimuthu, S., Arockiasamy, S., Terada, R. 2000. Genetic transformation of rice: current status and future prospects. Curr. Sci. 79 :186–195.

    Terada R. , 'Genetic transformation of rice: current status and future prospects ' (2000 ) 79 Curr. Sci. : 186 -195 .

    • Search Google Scholar

  • Jefferson, R.A., Kavanagh, T.A., Bevan, M.V. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6 :3901–3907.

    Bevan M.V. , 'GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants ' (1987 ) 6 EMBO J. : 3901 -3907 .

    • Search Google Scholar

  • Jiang, J.D., Linscombe, S.D., Wang, J.L., Oard, J.H. 2000. High efficiency transformation of US rice lines from mature seed-derived calli and segregation of glufosinate resistance under field conditions. Crop Sci. 40 :1729–1741.

    Oard J.H. , 'High efficiency transformation of US rice lines from mature seed-derived calli and segregation of glufosinate resistance under field conditions ' (2000 ) 40 Crop Sci. : 1729 -1741 .

    • Search Google Scholar

  • Jones, H.D., Angela, D., Wu, H.X. 2005. Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat. Plant Methods 1 :5.

    Wu H.X. , 'Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat ' (2005 ) 1 Plant Methods : 5 -.

    • Search Google Scholar

  • Liang, H., Zhu, Y.F., Zhu, Z., Sun, D.F., Jia, X. 2004. Obtainment of transgenic wheat with insecticidal lectin from snowdrop (Galanthus nivalis agglutinin, GNA) gene and analysis of resistance to aphids. Acta Genet. Sinica 31 :189–194.

    Jia X. , 'Obtainment of transgenic wheat with insecticidal lectin from snowdrop (Galanthus nivalis agglutinin, GNA) gene and analysis of resistance to aphids ' (2004 ) 31 Acta Genet. Sinica : 189 -194 .

    • Search Google Scholar

  • Khanna, H.K., Daggard, G.E. 2003. Agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium. Plant Cell Rep. 21 :429–436.

    Daggard G.E. , 'Agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium ' (2003 ) 21 Plant Cell Rep. : 429 -436 .

    • Search Google Scholar

  • Patnaik, D., Khurana, P. 2003. Genetic transformation of Indian bread (T. aestivum) and pasta (T. durum) wheat by particle bombardment of mature embryo-derived calli. BMC Plant Biology 3 :5–16.

    Khurana P. , 'Genetic transformation of Indian bread (T. aestivum) and pasta (T. durum) wheat by particle bombardment of mature embryo-derived calli ' (2003 ) 3 BMC Plant Biology : 5 -16 .

    • Search Google Scholar

  • Sambrook, J., Fritsch, E.F., Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press.

    Maniatis T. , '', in Molecular Cloning: A Laboratory Manual , (1989 ) -.

  • Shrawat, A.K, Lorz, H. 2006. Agrobacterium -mediated transformation of cereals: a promising approach crossing barriers. Plant Biotech. J. 4 :575–603.

    Lorz H. , 'Agrobacterium-mediated transformation of cereals: a promising approach crossing barriers ' (2006 ) 4 Plant Biotech. J. : 575 -603 .

    • Search Google Scholar

  • Sivamani, E., Shen, P., Opalka, N., Beachy, R.N., Fauquet, C.M. 1996. Selection of large quantities of embryogenic calli form indica rice seeds for production of fertile transgenic plants using the biolistic method. Plant Cell Rep. 15 :322–327.

    Fauquet C.M. , 'Selection of large quantities of embryogenic calli form indica rice seeds for production of fertile transgenic plants using the biolistic method ' (1996 ) 15 Plant Cell Rep. : 322 -327 .

    • Search Google Scholar

  • Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98 :503–517.

    Southern E.M. , 'Detection of specific sequences among DNA fragments separated by gel electrophoresis ' (1975 ) 98 J. Mol. Biol. : 503 -517 .

    • Search Google Scholar

  • Terada, R., Asao, H., Lida, S. 2004. A large-scale Agrobacterium -mediated transformation procedure with a strong positive-negative selection for gene targeting in rice ( Oryza sativa L.). Plant Cell Rep. 22 :653–659.

    Lida S. , 'A large-scale Agrobacterium-mediated transformation procedure with a strong positive-negative selection for gene targeting in rice (Oryza sativa L.) ' (2004 ) 22 Plant Cell Rep. : 653 -659 .

    • Search Google Scholar

  • Vasil, I.K., Vasil, V. 2006. Transformation of wheat via particle bombardment. Methods Mol. Biol. 318 : 273–283.

    Vasil V. , 'Transformation of wheat via particle bombardment ' (2006 ) 318 Methods Mol. Biol. : 273 -283 .

    • Search Google Scholar

  • Vasil, V., Castillo, A.M., Fromm, M.E., Vasil, I.K. 1992. Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10 :667–674.

    Vasil I.K. , 'Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus ' (1992 ) 10 Bio/Technology : 667 -674 .

    • Search Google Scholar

  • Vasil, V., Srivastava, V., Castillo, A.M. 1993. Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11 :1153–1158.

    Castillo A.M. , 'Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos ' (1993 ) 11 Bio/Technology : 1153 -1158 .

    • Search Google Scholar

  • Wu, H., Sparks, C., Amoah, B., Jones, H.D. 2003. Factors influencing successful Agrobacterium -mediated genetic transformation of wheat. Plant Cell Rep. 21 :659–668.

    Jones H.D. , 'Factors influencing successful Agrobacterium-mediated genetic transformation of wheat ' (2003 ) 21 Plant Cell Rep. : 659 -668 .

    • Search Google Scholar

  • Xu, Q.F., Li, L.C., Chen, X., Ma, Y.Z., Ye, X.G., Zhang, Z.Y., Xu, H.J., Xin, Z.Y. 2001. Study on the obtaining of transgenic wheat with GNA alien gene by biolistic particle. Sci. Agricult. Sinica 34 :5–8.

    Xin Z.Y. , 'Study on the obtaining of transgenic wheat with GNA alien gene by biolistic particle ' (2001 ) 34 Sci. Agricult. Sinica : 5 -8 .

    • Search Google Scholar

  • Ye, X.G., Chen, H.M., Xu, H.J., Du, L.P., Lu, W.Z., Huang, Y.H. 2005. Development of transgenic wheat plants with chitinase and β -1,3-glucanase genes and their resistance to Fusarium head blight. Acta Agron. Sinica 31 :583–586.

    Huang Y.H. , 'Development of transgenic wheat plants with chitinase and β-1,3-glucanase genes and their resistance to Fusarium head blight ' (2005 ) 31 Acta Agron. Sinica : 583 -586 .

    • Search Google Scholar

  • Zhang, Z., Xing, A., Staswick, P., Clemente, T. 1999. The use of glufosinate as a selective agent in Agrobacterium -mediated transformation of soybean. Plant Cell Tiss. Org. Cult. 56 :37–46.

    Clemente T. , 'The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean ' (1999 ) 56 Plant Cell Tiss. Org. Cult. : 37 -46 .

    • Search Google Scholar

  • Zhou, H., Berg, J.D., Blank, S.E., Chay, C.A., Chen, G., Eskelsen, S.R. 2003. Field efficacy assessment of transgenic roundup ready wheat. Crop Sci. 43 :1072–1075.

    Eskelsen S.R. , 'Field efficacy assessment of transgenic roundup ready wheat ' (2003 ) 43 Crop Sci. : 1072 -1075 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

 

 

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Mar 2024 13 0 0
Apr 2024 9 0 0
May 2024 14 0 0
Jun 2024 16 0 0
Jul 2024 26 0 0
Aug 2024 33 0 0
Sep 2024 9 0 0