Cereal Research Communications
Volume/Issue:
Volume 37: Issue 1
Development and application of EST-STS markers specific to chromosome 1RS of Secale cereale
Authors:
C. Wang Chinese Academy of Sciences Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology Shijiazhuang 050021 China

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L. Li Chinese Academy of Agricultural Sciences Institute of Crop Science Beijing 100081 China

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X. Zhang Chinese Academy of Sciences Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology Shijiazhuang 050021 China

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Q. Gao Chinese Academy of Sciences Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology Shijiazhuang 050021 China

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R. Wang Chinese Academy of Sciences Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology Shijiazhuang 050021 China

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D. An Chinese Academy of Sciences Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology Shijiazhuang 050021 China

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Pages:
13–21
Online Publication Date:
05 Mar 2009
Publication Date:
01 Mar 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.37.2009.1.2
Keywords:
wheat; expressed sequence tag (EST); sequence-tagged site (STS); Secale cereale ; chromosome specific marker; marker-assisted selection (MAS)
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Molecular markers are important tools that have been used to identify the short arm of rye chromosome 1R (1RS) which contains many useful genes introgressed into wheat background. Wheat expressed sequence tag (EST) sequences are valuable for developing molecular markers since ESTs are derived from gene transcripts and more likely to be conserved between wheat and its relative species. In the present study, 35 sequence-tagged site (STS) primers were designed based on EST sequences distributed on homology group 1 chromosomes of Triticum aestivum and used to screen specific markers for chromosome 1RS of Secale cereale . Two primer pairs different from the early studies, STS WE3 , which amplified a 1680-bp and a 1750-bp fragment, and STS WE126 , which produced a 850-bp fragment from rye genome, were proved to be specific to chromosome 1RS since the corresponding fragments were only amplified from 1R chromosome addition line and wheat-rye lines with chromosome 1RS, but not from wheat-rye 2R-7R chromosome addition lines and the other lines lacking chromosome 1RS. Eleven wheat-rye lines derived from ‘Xiaoyan 6’ and ‘German White’ were used to test the presence of specific markers for 1RS. The specific fragments of 1RS were amplified in 4 wheat-rye lines, but not in the other lines. The testing results using EST-STS markers of 1RS were consistent with those obtained from fluorescence in situ hybridization (FISH), suggesting that these markers specific to 1RS could be used in marker-assisted selection (MAS) for incorporating 1RS into wheat cultivars in breeding.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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