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  • 1 University of Tehran Department of Horticultural Science, Agricultural College Karaj Iran
  • 2 The University of Sydney Plant Breeding Institute PMB 11 Camden NSW 2570 Australia
  • 3 Value Added Wheat CRC Limited Locked Bag 1345 North Ryde NSW 1670 Australia
  • 4 University of Tehran Agronomy and Plant Breeding Department, Agricultural College Karaj Iran
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A RFLP approach was used to investigate polymorphism of ω -gliadin genes in Ae. tauschii using a F 2 population from the cross of accessions AUS18913 and CPI110856. A set of 150 F 2 progenies was genotyped by acid polyacrylamide gel electrophoresis (A-PAGE) and only one recombinant line of Gli-Dt1/Gli-DtT1 was observed. Twelve restriction enzymes were initially tested on genomic DNA of the two parents of which four restriction enzymes revealed polymorphism. Of these four, only Dra I was associated with the novel ω -gliadin gene (T 1 ) using a 1,200 bp DNA fragment of a ω -gliadin gene as a gene-specific probe. The ω -gliadin gene (T 1 ) may be of interest for further studies relating storage proteins and wheat bread-making quality.

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