Cereal Research Communications
Volume/Issue:
Volume 37: Issue 2
Transposon-derived organ-specific mutation in the ADH1 RNA 5′ untranslated leader of autotetraploid maize ( Zea mays L.)
Authors:
M. Hajós-Novák Szent István University (SZIE) Institute for Genetics and Biotechnology, Section of Plant Breeding Páter K. u. 1 H-2100 Gödöllő Hungary

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G. Dallmann Agricultural Biotechnological Research Centre (MBK) Szentgyörgyi A. u. 4 H-2100 Gödöllő Hungary

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Z. Kristóf Eötvös Loránd University (ELTE) Department of Anatomy Pázmány s. 1/C H-1117 Budapest Hungary

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Pages:
151–158
Online Publication Date:
28 May 2009
Publication Date:
01 Jun 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.37.2009.2.1
Keywords:
Ds1 element; Adh1 ; autotetraploid maize; unstable organ-specific mutation; 5′ UTR; point mutation
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A new unstable organ-specific Adh1 mutant was isolated from autotetraploid S 25 Wf9 maize line after germinating kernels under water for two days. Adh1-Fm4x-1 expresses normal and/or reduced levels of ADH1 in anaerobic scutellum. No activity or approximately 33–75% of wild type level of ADH1 in pollen grains was observed. The organ-specific phenotype of the Adh1-Fm4x-1 could be maintained by selfing for ten subsequent generations. After crossing with the wild-type allele the Adh1 mutation transmitted to the next generation both by female and male gametophytes. The appearance of one- and two-banded patterns in anaerobic scutellum and pollen grains of heterozygous F 1 plants showed the lack of F·F homodimers.DNA sequence analyses of the wild type allele, the Adh1-Fm4x-1 mutant allele and four F 1 revertants revealed that the Adh1-Fm4x-1 mutation contains a Dissociation (Ds1) transposable element in the 5′ untranslated leader of the maize Adh1 gene, which regulates organ-specific expression at a quantitative level.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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