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  • 1 Szent István University (SZIE) Institute for Genetics and Biotechnology, Section of Plant Breeding Páter K. u. 1 H-2100 Gödöllő Hungary
  • 2 Agricultural Biotechnological Research Centre (MBK) Szentgyörgyi A. u. 4 H-2100 Gödöllő Hungary
  • 3 Eötvös Loránd University (ELTE) Department of Anatomy Pázmány s. 1/C H-1117 Budapest Hungary
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A new unstable organ-specific Adh1 mutant was isolated from autotetraploid S 25 Wf9 maize line after germinating kernels under water for two days. Adh1-Fm4x-1 expresses normal and/or reduced levels of ADH1 in anaerobic scutellum. No activity or approximately 33–75% of wild type level of ADH1 in pollen grains was observed. The organ-specific phenotype of the Adh1-Fm4x-1 could be maintained by selfing for ten subsequent generations. After crossing with the wild-type allele the Adh1 mutation transmitted to the next generation both by female and male gametophytes. The appearance of one- and two-banded patterns in anaerobic scutellum and pollen grains of heterozygous F 1 plants showed the lack of F·F homodimers.DNA sequence analyses of the wild type allele, the Adh1-Fm4x-1 mutant allele and four F 1 revertants revealed that the Adh1-Fm4x-1 mutation contains a Dissociation (Ds1) transposable element in the 5′ untranslated leader of the maize Adh1 gene, which regulates organ-specific expression at a quantitative level.

  • Chen, C.-H., Freeling, M., Mercklebach, A. 1986. Enzymatic and morphological consequences of Ds excisions from maize Adh1 . Maydica (McClintock issue) 31 :93–108.

    Mercklebach A. , 'Enzymatic and morphological consequences of Ds excisions from maize Adh1 ' (1986 ) 31 Maydica (McClintock issue) : 93 -108.

    • Search Google Scholar
  • Chen, C.-H., Olshi, K.K., Kloeckener-Gruissem, B., Freeling, M. 1987. Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics 116 :469–477.

    Freeling M. , 'Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion ' (1987 ) 116 Genetics : 469 -477.

    • Search Google Scholar
  • Davis, R.J. 1964. Disc electrophoresis 2. Method of application to human serum proteins. A.N.Y. Acid. Sci. 121 :404–427.

    Davis R.J. , 'Disc electrophoresis 2. Method of application to human serum proteins ' (1964 ) 121 A.N.Y. Acid. Sci. : 404 -427.

    • Search Google Scholar
  • Dawe, R.K., Lachmansingh, A.R., Freeling, M. 1993. Transposon-mediated mutations in the untranslated leader of maize Adh1 that increase and decrease pollen-specific gene expression. Plant Cell 5 :311–319.

    Freeling M. , 'Transposon-mediated mutations in the untranslated leader of maize Adh1 that increase and decrease pollen-specific gene expression ' (1993 ) 5 Plant Cell : 311 -319.

    • Search Google Scholar
  • Freeling, M. 1976. Intragenic recombination in maize: Pollen analysis methods and the effect of parental Adh1 isoalleles. Genetics 83 :701–717.

    Freeling M. , 'Intragenic recombination in maize: Pollen analysis methods and the effect of parental Adh1 isoalleles ' (1976 ) 83 Genetics : 701 -717.

    • Search Google Scholar
  • Freeling, M., Cheng, S.-K.D., Alleman, M. 1982. Mutant alleles that are altered in quantitative, organ-specific behavior. Dev. Genet. 3 :179–196.

    Alleman M. , 'Mutant alleles that are altered in quantitative, organ-specific behavior ' (1982 ) 3 Dev. Genet. : 179 -196.

    • Search Google Scholar
  • Hajósné, Novák M., H. Nagy, A. 1995. Az alkohol dehidrogenáz-1 (Adh1) gén kifejeződése tetraploid kukorica ( Zea mays L.) pollenjében. [Expression of alcohol-dehydrogenase-1 ( Adh1 ) gene in the pollen grains of autotetraploid maize ( Zea mays L.)]. Növénytermelés 44 :229–235.

    H. Nagy A. , 'Az alkohol dehidrogenáz-1 (Adh1) gén kifejeződése tetraploid kukorica (Zea mays L.) pollenjében. [Expression of alcohol-dehydrogenase-1 (Adh1) gene in the pollen grains of autotetraploid maize (Zea mays L.)] ' (1995 ) 44 Növénytermelés : 229 -235.

    • Search Google Scholar
  • Kloeckner-Guissem, B., Vogel, J.M., Freeling, M. 1992. The TATA box promoter region of maize Adh1 affects its organ-specific expression. The EMBO Journal 11 :157–166.

    Freeling M. , 'The TATA box promoter region of maize Adh1 affects its organ-specific expression ' (1992 ) 11 The EMBO Journal : 157 -166.

    • Search Google Scholar
  • Schwartz, D. 1969. An example of gene fixation resulting from selective advantage in suboptimal conditions. Am. Nat. 103 :479–481.

    Schwartz D. , 'An example of gene fixation resulting from selective advantage in suboptimal conditions ' (1969 ) 103 Am. Nat. : 479 -481.

    • Search Google Scholar
  • Schwartz, D., Osterman, J. 1976. A pollen selection system for alcohol-dehydrogenase negative mutants in plants. Genetics 83 :63–65.

    Osterman J. , 'A pollen selection system for alcohol-dehydrogenase negative mutants in plants ' (1976 ) 83 Genetics : 63 -65.

    • Search Google Scholar
  • Sutton, W.D., Gerlach, W.L., Schwartz, D., Peacock, W.J. 1984. Molecular nature of Ds controlling element mutations at the Adh1 locus of maize. Science 223 :1265–1268.

    Peacock W.J. , 'Molecular nature of Ds controlling element mutations at the Adh1 locus of maize ' (1984 ) 223 Science : 1265 -1268.

    • Search Google Scholar
  • Williams, C.E., Kloeckener-Guissem, B., Freeling, M. 1991. Naturally occurring variants of maize Adh1 differ in organ-specific expression both in quantity and developmental timing. Maydica 36 :115–128.

    Freeling M. , 'Naturally occurring variants of maize Adh1 differ in organ-specific expression both in quantity and developmental timing ' (1991 ) 36 Maydica : 115 -128.

    • Search Google Scholar