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  • 1 Chinese Academy of Agricultural Sciences (CAAS) Institute of Crop Sciences/National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement 12 Zhongguancun South Street Beijing 100081 China
  • 2 Inner Mongolia Agricultural University College of Biology Engineering 306 Zhaowuda Road Hohhot, Inner Mongolia 010018 China
  • 3 CIMMYT China Office, C/O CAAS Beijing 100081 China
  • 4 Leibiniz Institute of Plant Genetics and Crop Plant Research (IPK) Corrensstrasse 3 Gatersleben D-06466 Germany
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Genotypes with various Vp-1B alleles perform different levels of pre-harvest sprouting (PHS) tolerance. In this study, 217 white-grained wheat cultivars, including 75 landraces, 39 historical cultivars, and 103 modern cultivars from five major regions of China, were examined to characterize the diversity of the Viviparous-1B ( Vp-1B ) locus associated with PHS tolerance. Four Vp-1B alleles were identified, three ( Vp-1Ba , Vp-1Bb and Vp-1Bc ) of which were previously reported in Chinese wheat cultivars. A new allele, Vp-1Be , was identified in the PHS tolerant landrace Hongheshangtou. Sequence analysis showed that Vp-1Be had an insertion of a 4-bp fragment, two SNPs, and a deletion of an 83-bp fragment compared with the nucleotide sequence of Vp-1Ba (AJ400713), all located in the third intron. Vp-1Be shared 97.80% similarity with the nucleotide sequence of AJ400713. The frequencies of Vp-1Ba , Vp-1Bb , and Vp-1Bc were 36.0%, 5.3%, and 57.3% in landraces; 23.1%, 7.7%, and 69.2% in historical cultivars; and 52.4%, 0%, and 47.6% in current cultivars, respectively.

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