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  • 1 Institute of Soil Science and Plant Cultivation Department of Biochemistry ul. Czartoryskich 8 24-100 Puławy Poland
  • 2 Institute of Soil Science and Plant Cultivation Department of Systems and Economics of Crop Production ul. Czartoryskich 8 24-100 Puławy Poland
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Epidemiological data suggest that consumption of whole-grain and bran may help to prevent cardiovascular diseases, diabetes and certain forms of cancer. The beneficial health effects are usually attributed to the presence of dietary fibre and bioactive secondary metabolites, including phenolic acids. Wheat is an important component of the human diet and may be a significant source of phenolic antioxidants. To date, few studies have investigated the effect of various agricultural practices on levels of secondary metabolites in crops. The aim of this work was to determine the phenolic acid content in four winter wheat cultivars, grown using conventional and organic agricultural practices. Five phenolic acids were detected by HPLC analyses. Ferulic acid was the predominant phenolic acid in the grain of all tested wheat varieties. The remaining phenolic acids, i.e. sinapic acid, p -coumaric acid, vanillic acid, and p -hydroxybenzoic acid, were present in considerably lower amounts. Significant differences among cultivars in concentration of particular phenolic acids, as well as in the total phenolic acid content were observed. The effect of various agricultural practices on phenolic acid levels in wheat grains was also analysed. Organically grown plants are usually considered to contain more secondary metabolites. In this study, however, organic agriculture did not lead to a significant increase in phenolic acids. Only a small, statistically irrelevant trend towards higher levels of phenolic acids in organic wheat samples was demonstrated.

  • Adom, K.K., Sorrells, M.E., Liu, R.H. 2003. Phytochemical profiles and antioxidant activity of wheat varieties. J. Agric. Food Chem. 51 :7825–7834.

    Liu R.H. , 'Phytochemical profiles and antioxidant activity of wheat varieties ' (2003 ) 51 J. Agric. Food Chem. : 7825 -7834.

    • Search Google Scholar
  • Andreasen., M.F., Kroon, P.A., Williamson, G., Garcia-Conesa, M.-T. 2001. Esterase activity able to hydrolyse dietary antioxidant hydroxycinnamates is distributed along the intestine of mammals. J. Agric. Food Chem. 49 :5679–5684.

    Garcia-Conesa M.-T. , 'Esterase activity able to hydrolyse dietary antioxidant hydroxycinnamates is distributed along the intestine of mammals ' (2001 ) 49 J. Agric. Food Chem. : 5679 -5684.

    • Search Google Scholar
  • Asami, D.K, Hong, Y.-J., Barret, D.M., Mitchell, A.E. 2003. Comparison of the total phenolic and ascorbic acid content of freeze-dried and air dried marionberry, strawberry and corn using conventional, organic and sustainable agricultural practices. J. Agric. Food Chem. 52 :1237–1241.

    Mitchell A.E. , 'Comparison of the total phenolic and ascorbic acid content of freeze-dried and air dried marionberry, strawberry and corn using conventional, organic and sustainable agricultural practices ' (2003 ) 52 J. Agric. Food Chem. : 1237 -1241.

    • Search Google Scholar
  • Baublis, A.J., Lu, C., Clydesdale, F.M., Decker, E.A. 2000. Potential of wheat-based breakfast cereals as a source of dietary antioxidants. J. Am. Coll. Nutr. 19 :308–311.

    Decker E.A. , 'Potential of wheat-based breakfast cereals as a source of dietary antioxidants ' (2000 ) 19 J. Am. Coll. Nutr. : 308 -311.

    • Search Google Scholar
  • Brandt, K., Mølgaard, J.P. 2001. Organic agriculture: Does it enhance or reduce the nutritional value of plant foods? J. Sci. Food Agric. 81 :924–931.

    Mølgaard J.P. , 'Organic agriculture: Does it enhance or reduce the nutritional value of plant foods? ' (2001 ) 81 J. Sci. Food Agric. : 924 -931.

    • Search Google Scholar
  • Dimberg, L.H., Gissén, N., Nilsson, J. 2005. Phenolic compounds in oat grains ( Avena sativa L.) grown in conventional and organic systems. Ambio 34 :331–337.

    Nilsson J. , 'Phenolic compounds in oat grains (Avena sativa L.) grown in conventional and organic systems ' (2005 ) 34 Ambio : 331 -337.

    • Search Google Scholar
  • Dixon, R.A., Paiva, N.L. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7 :1085–1097.

    Paiva N.L. , 'Stress-induced phenylpropanoid metabolism ' (1995 ) 7 Plant Cell : 1085 -1097.

  • Estiarte, M., Filella, I., Serra, J., Peñuelas, J. 1994. Effects of nutrient and water stress on leaf phenolic content of peppers and susceptibility to generalist herbivore Helicoverpa armigera (Hubner). Oecologia 99 :387–391.

    Peñuelas J. , 'Effects of nutrient and water stress on leaf phenolic content of peppers and susceptibility to generalist herbivore Helicoverpa armigera (Hubner) ' (1994 ) 99 Oecologia : 387 -391.

    • Search Google Scholar
  • Häkkinen, S.H., Törrönen A.R. 2000. Content of flavonols and selected phenolic acids in strawberries and Vaccinium species: influence of cultivar, cultivation site and technique. Food Res. Int. 33 :517–524.

    Törrönen A.R. , 'Content of flavonols and selected phenolic acids in strawberries and Vaccinium species: influence of cultivar, cultivation site and technique ' (2000 ) 33 Food Res. Int. : 517 -524.

    • Search Google Scholar
  • Heiniö, R.-L., Liukkonen, K.-H., Myllymäki, O., Pihlava, J.-M., Adlercreutz, H., Heinonen, S.-M., Poutanen, K. 2008. Quantities of phenolic compounds and their impacts on the perceived flavour attributes of rye grain. J. Cereal Sci. 47 :566–575.

    Poutanen K. , 'Quantities of phenolic compounds and their impacts on the perceived flavour attributes of rye grain ' (2008 ) 47 J. Cereal Sci. : 566 -575.

    • Search Google Scholar
  • Jacobs, D.R., Slavin, J., Marquart, L. 1995. Whole grain intake and cancer: a review of the literature. Nutr. Cancer 24 :221–229.

    Marquart L. , 'Whole grain intake and cancer: a review of the literature ' (1995 ) 24 Nutr. Cancer : 221 -229.

    • Search Google Scholar
  • Jacobs, D.R. Jr., Meyer, K.A., Kushi, L.H., Folsom, A.R. 1998. Whole grain intake may reduce risk of coronary heart disease death in postmenopausal women: The Iowa women’s health study. Am. J. Clin. Nutr. 68 : 248–257.

    Folsom A.R. , 'Whole grain intake may reduce risk of coronary heart disease death in postmenopausal women: The Iowa women’s health study ' (1998 ) 68 Am. J. Clin. Nutr. : 248 -257.

    • Search Google Scholar
  • Kovačik, J., Klejdus, B., Bačkor, M., Repčák, M. 2007. Phenylalanine ammonia-lyase activity and phenolic compounds accumulation in nitrogen-deficient Matricaria chamomilla leaf rosettes. Plant Sci. 172 :393–399.

    Repčák M. , 'Phenylalanine ammonia-lyase activity and phenolic compounds accumulation in nitrogen-deficient Matricaria chamomilla leaf rosettes ' (2007 ) 172 Plant Sci. : 393 -399.

    • Search Google Scholar
  • Kroon, P.A., Faulds, C.B., Ryden, P., Robertson, J.A., Williamson, G. 1997. Release of covalently bound ferulic acid from fiber in the human colon. J. Agric. Food Chem. 45 :661–667.

    Williamson G. , 'Release of covalently bound ferulic acid from fiber in the human colon ' (1997 ) 45 J. Agric. Food Chem. : 661 -667.

    • Search Google Scholar
  • Lempereur, I., Rouau, X., Abecassis, J. 1997. Genetic and agronomic variation in arabinoxylan and ferulic acid contents of durum wheat ( Triticum durum L.) grain and its milling fractions. J. Cereal Sci. 25 :103–110.

    Abecassis J. , 'Genetic and agronomic variation in arabinoxylan and ferulic acid contents of durum wheat (Triticum durum L.) grain and its milling fractions ' (1997 ) 25 J. Cereal Sci. : 103 -110.

    • Search Google Scholar
  • Li, L., Shewry, P.R., Ward, J.R. 2008. Phenolic acids in wheat varieties in HEALTHGRAIN diversity screen. J. Agric. Food Chem. 56 :9732–9739.

    Ward J.R. , 'Phenolic acids in wheat varieties in HEALTHGRAIN diversity screen ' (2008 ) 56 J. Agric. Food Chem. : 9732 -9739.

    • Search Google Scholar
  • Liyana-Pathirana, C.M., Shahidi, F. 2006. Importance of insoluble-bound phenolics to antioxidant properties of wheat. J. Agric. Food Chem. 54 :1256–1264.

    Shahidi F. , 'Importance of insoluble-bound phenolics to antioxidant properties of wheat ' (2006 ) 54 J. Agric. Food Chem. : 1256 -1264.

    • Search Google Scholar
  • Manach, C., Scalbert, A., Morand, C., Rémésy, C., Jiménez, L. 2004. Polyphenols: food resources and bioavailability. Am. J. Clin. Nutr. 79 :727–747.

    Jiménez L. , 'Polyphenols: food resources and bioavailability ' (2004 ) 79 Am. J. Clin. Nutr. : 727 -747.

    • Search Google Scholar
  • Meyer, K.A., Kushi, L.H., Jacobs, D.R. Jr., Slavin, J., Sellers, T.A., Folsom, A.R. 2000. Carbohydrates, dietary fiber, incident type 2 diabetes in older women. Am. J. Clin. Nutr. 71 :921–930.

    Folsom A.R. , 'Carbohydrates, dietary fiber, incident type 2 diabetes in older women ' (2000 ) 71 Am. J. Clin. Nutr. : 921 -930.

    • Search Google Scholar
  • Moore, J., Hao, Z., Zhou, K., Luther, M., Costa, J., Yu, L. 2005. Carotenoid, tocopherol, phenolic acid and antioxidant properties of Maryland-grown soft wheat. J. Agric. Food Chem. 53 :6649–6657.

    Yu L. , 'Carotenoid, tocopherol, phenolic acid and antioxidant properties of Maryland-grown soft wheat ' (2005 ) 53 J. Agric. Food Chem. : 6649 -6657.

    • Search Google Scholar
  • Mpofu, A., Sapirstein, H.D., Beta, T. 2006. Genotype and environmental variation in phenolic content, phenolic acid composition and antioxidant activity of hard spring wheat. J. Agric. Food Chem. 54 :1265–1270.

    Beta T. , 'Genotype and environmental variation in phenolic content, phenolic acid composition and antioxidant activity of hard spring wheat ' (2006 ) 54 J. Agric. Food Chem. : 1265 -1270.

    • Search Google Scholar
  • Naczk, M., Shahidi, F. 2006. Phenolics in cereals, fruits and vegetables: occurrence, extraction and analysis. J. Pharm. Biomed. Anal. 41 :1523–1542.

    Shahidi F. , 'Phenolics in cereals, fruits and vegetables: occurrence, extraction and analysis ' (2006 ) 41 J. Pharm. Biomed. Anal. : 1523 -1542.

    • Search Google Scholar
  • Nurmi, T., Nyström, L., Edelmann, M., Lampi, A.-M., Pironen, V. 2008. Phytosterols in wheat genotypes in the HEALTHGRAIN diversity screen. J. Agric. Food Chem. 56 :9710–9715.

    Pironen V. , 'Phytosterols in wheat genotypes in the HEALTHGRAIN diversity screen ' (2008 ) 56 J. Agric. Food Chem. : 9710 -9715.

    • Search Google Scholar
  • Ransom, J.K., Berzonsky, W.A., Sorenson, B.K. 2006. Hard white wheat — Producing North Dakota’s next market opportunity. NDSU Extension Service, Fargo, A-1310.

    Sorenson B.K. , '', in Hard white wheat — Producing North Dakota’s next market opportunity , (2006 ) -.

    • Search Google Scholar
  • Siebenhandl, S., Grausgruber, H., Pellegrini, N., del Rio, D., Fogliano, V., Pernice, R., Berghofer, E. 2007. Phytochemical profile of main antioxidants in different fractions of purple and blue wheat, and black barley. J. Agric. Food Chem. 55 :8541–8547.

    Berghofer E. , 'Phytochemical profile of main antioxidants in different fractions of purple and blue wheat, and black barley ' (2007 ) 55 J. Agric. Food Chem. : 8541 -8547.

    • Search Google Scholar
  • Sosulski, F., Krygier, K., Hogge, L. 1982. Free, esterified and insoluble-bound phenolic acids. 3. Composition of phenolic acids in cereal and potato flours. J. Agric. Food Chem. 30 :337–340.

    Hogge L. , 'Free, esterified and insoluble-bound phenolic acids. 3. Composition of phenolic acids in cereal and potato flours ' (1982 ) 30 J. Agric. Food Chem. : 337 -340.

    • Search Google Scholar
  • Winter, C.K., Davis, S.F. 2006. Organic foods. J. Food Sci. 71 :R117–R124.

    Davis S.F. , 'Organic foods ' (2006 ) 71 J. Food Sci. : R117 -R124.

  • Worthington, W. 2001. Nutritional quality of organic versus conventional fruits, vegetables and grains. J. Altern. Complement. Med. 7 :161–173.

    Worthington W. , 'Nutritional quality of organic versus conventional fruits, vegetables and grains ' (2001 ) 7 J. Altern. Complement. Med. : 161 -173.

    • Search Google Scholar
  • Zhou, K., Laux, J.J., Yu, Y. 2004. Comparison of Swiss red wheat grain and fractions for their antioxidant properties. J. Agric. Food Chem. 52 :1118–1123.

    Yu Y. , 'Comparison of Swiss red wheat grain and fractions for their antioxidant properties ' (2004 ) 52 J. Agric. Food Chem. : 1118 -1123.

    • Search Google Scholar