Cereal Research Communications
Volume/Issue:
Volume 37: Issue 3
Analysis of cpSSR in triticale plants obtained by in vitro androgenesis
Authors:
J. González University of Alcalá Department of Cell Biology and Genetics, Campus Universitario 28871 Alcalá de Henares (Madrid) Spain

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P. Rubio University of Alcalá Department of Cell Biology and Genetics, Campus Universitario 28871 Alcalá de Henares (Madrid) Spain

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A. López University of Alcalá Department of Cell Biology and Genetics, Campus Universitario 28871 Alcalá de Henares (Madrid) Spain

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N. Jouve University of Alcalá Department of Cell Biology and Genetics, Campus Universitario 28871 Alcalá de Henares (Madrid) Spain

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Pages:
345–352
Online Publication Date:
08 Sep 2009
Publication Date:
01 Sep 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.37.2009.3.3
Keywords:
albino plants; Triticale; cpSSR; double haploids; microsatellites; androgenesis
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In vitro androgenesis allows haploid and double haploid homozygous plants to be obtained. However, in cereals, non-viable albino plants are often produced. This limits the use of this technique both in basic studies and in plant genetic improvement programs. We have analyzed eight microsatellites of the chloroplast genome (cpSSR) in a collection of 46 double haploid green lines derived from the intervarietal hybrid ‘Torote’ × ‘Presto’, and 49 haploid albino plants all obtained from the double haploid line ‘ATOPE-41’, in order to know the stability of this sequences after the androgenetic process. The green plants showed no variations at the analyzed loci, while two of the albino plants differed at two loci: WCt2 and WCt9. These variations notably affected the microsatellite nucleotide sequences as well as the sequences surrounding them. The changes observed consisted of insertions, deletions and nucleotide substitutions, with transition and transversion types equally common. The latter albino plants showed several alleles at the polymorphic loci, indicating the existence of heteroplasmy.

  • Abe, T., Li, N., Togashi, A., Sasahara, T. 2002. Large deletions in chloroplast DNA of rice calli after long-term culture. J. Plant Physiol. 159 : 917–923.

    Sasahara T. , 'Large deletions in chloroplast DNA of rice calli after long-term culture ' (2002 ) 159 J. Plant Physiol. : 917 -923 .

    • Search Google Scholar

  • Caredda, S., Clément, C. 1999. Androgenesis and albinism in Poaceae: influence of genotype and carbohydrates. In: Clément, C., Pacini, E., Andran, J.C. (eds), Anther and Pollen: From Biology to Biotechnology. Springer Verlag, Berlin, Heidelberg, Tokyo, pp. 211, 228.

    Clément C. , '', in Anther and Pollen: From Biology to Biotechnology , (1999 ) -.

  • Caredda, S., Devaux, P., Sangwan, R.S., Clément, C. 1999. Differential development of plastids during microspore embryogenesis in barley. Protoplasma. 208 :248–256.

    Clément C. , 'Differential development of plastids during microspore embryogenesis in barley ' (1999 ) 208 Protoplasma : 248 -256 .

    • Search Google Scholar

  • Caredda, S., Devaux, P., Sangwan, R.S., Proult, I., Clément, C. 2004. Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley (Hordeum vulgare) cultivars. Plant Cell, Tiss. Organ Cult. 76 :35–43.

    Clément C. , 'Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley (Hordeum vulgare) cultivars ' (2004 ) 76 Plant Cell, Tiss. Organ Cult. : 35 -43 .

    • Search Google Scholar

  • Caredda, S., Doncoeur, C., Devaux, P., Sangwan, R.S., Clément, C. 2000. Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley ( Hordeum vulgare L.). Sex Plant Reprod. 13 :95–104.

    Clément C. , 'Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.) ' (2000 ) 13 Sex Plant Reprod : 95 -104 .

    • Search Google Scholar

  • Chen, X., Cho, Y.G., McCouch, S.R. 2002. Sequence divergence of rice microsatellites in Oryza and other plant species. Mol. Genet. Genomics 268 :331–343.

    McCouch S.R. , 'Sequence divergence of rice microsatellites in Oryza and other plant species ' (2002 ) 268 Mol. Genet. Genomics : 331 -343 .

    • Search Google Scholar

  • Day, A., Ellis, T.H.N. 1984. Chloroplast DNA deletions associated with wheat plants regenerated from pollen: Possible basis for maternal inheritance of chloroplasts. Cell 39 :359–368.

    Ellis T.H.N. , 'Chloroplast DNA deletions associated with wheat plants regenerated from pollen: Possible basis for maternal inheritance of chloroplasts ' (1984 ) 39 Cell : 359 -368 .

    • Search Google Scholar

  • Dunford, R., Walden, R.M. 1991. Plastid genome structure and plastid-related transcript levels in albino barley plants derived from anther culture. Curr. Genet. 20 :339–347.

    Walden R.M. , 'Plastid genome structure and plastid-related transcript levels in albino barley plants derived from anther culture ' (1991 ) 20 Curr. Genet. : 339 -347 .

    • Search Google Scholar

  • González, J.M., Hernández, I., Jouve, N. 1997. Analysis of anther culture response in hexaploid triticale. Plant Breeding 116 :302–304.

    Jouve N. , 'Analysis of anther culture response in hexaploid triticale ' (1997 ) 116 Plant Breeding : 302 -304 .

    • Search Google Scholar

  • González, J.M., Jouve, N. 2000. Improvement of anther culture media for haploid production in triticale. Cereal Res. Commun. 28 :65–72.

    Jouve N. , 'Improvement of anther culture media for haploid production in triticale ' (2000 ) 28 Cereal Res. Commun. : 65 -72 .

    • Search Google Scholar

  • González, J.M., Muñiz, L.M., Jouve, N. 2005. Mapping of Qtls for androgenetic response based on a molecular genetic map of × Triticosecale Wittmack. Genome 48 :999–1009.

    Jouve N. , 'Mapping of Qtls for androgenetic response based on a molecular genetic map of × Triticosecale Wittmack ' (2005 ) 48 Genome : 999 -1009 .

    • Search Google Scholar

  • Harada, T., Sato, T., Asaka, D., Matsukawa, I. 1991. Large-scale deletion of rice plastid DNA in anther culture. Theor. Appl. Genet. 81 :157–161.

    Matsukawa I. , 'Large-scale deletion of rice plastid DNA in anther culture ' (1991 ) 81 Theor. Appl. Genet. : 157 -161 .

    • Search Google Scholar

  • Horlow, C., Raquin, C. 1998. A critical analysis of existing haploidization techniques. In: Chupeau, Caboche, Henri (eds), Androgenesis and Haploid Plants. INRA/Springer Verlag, pp. 7–23.

  • Ishii, T., Mori, N., Ogihara, Y. 2001. Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species. Theor. Appl. Genet. 103 :896–904.

    Ogihara Y. , 'Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species ' (2001 ) 103 Theor. Appl. Genet. : 896 -904 .

    • Search Google Scholar

  • Jain, S.M. 2001. Tissue culture-derived variation in crop improvement. Euphytica 118 :153–166.

    Jain S.M. , 'Tissue culture-derived variation in crop improvement ' (2001 ) 118 Euphytica : 153 -166 .

    • Search Google Scholar

  • Jacobsson, M., Säll, T., Lind-Halldén, C., Halldén, C. 2007. Evolution of chloroplast mononucleotide microsatellites in Arabidopsis thaliana . Theor. Appl. Genet. 114 :223–235.

    Halldén C. , 'Evolution of chloroplast mononucleotide microsatellites in Arabidopsis thaliana ' (2007 ) 114 Theor. Appl. Genet. : 223 -235 .

    • Search Google Scholar

  • Larkin, P.J., Scowcroft, W.R. 1981. Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60 :197–214.

    Scowcroft W.R. , 'Somaclonal variation — a novel source of variability from cell cultures for plant improvement ' (1981 ) 60 Theor. Appl. Genet. : 197 -214 .

    • Search Google Scholar

  • Larsen, E.T., Tuvesson, I.K.D., Andersen, S.B. 1991. Nuclear genes affecting percentage of green plants in barley ( Hordeum vulgare L.) anther culture. Theor. Appl. Genet. 82 :417–420.

    Andersen S.B. , 'Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.) anther culture ' (1991 ) 82 Theor. Appl. Genet. : 417 -420 .

    • Search Google Scholar

  • Liu, N.T., Jane, W.N., Tsay, H.S., Wu, H., Chang, W.C., Lin, C.S. 2007. Chloroplast genome aberration in micropropagation-derived albino Bambusa edulis mutants, ab1 and ab2. Plant Cell, Tiss. Organ Cult. 88 :147–156.

    Lin C.S. , 'Chloroplast genome aberration in micropropagation-derived albino Bambusa edulis mutants, ab1 and ab2. Plant Cell ' (2007 ) 88 Tiss. Organ Cult. : 147 -156 .

    • Search Google Scholar

  • Matsuoka, Y., Yamazaki, Y., Ogihara, Y., Tsunewaki, K. 2002. Whole chloroplast genome comparison of rice, maize and wheat: implications for chloroplast gene diversification and phylogeny of cereals. Mol. Biol. Evo. 19 :2084–2091.

    Tsunewaki K. , 'Whole chloroplast genome comparison of rice, maize and wheat: implications for chloroplast gene diversification and phylogeny of cereals ' (2002 ) 19 Mol. Biol. Evo. : 2084 -2091 .

    • Search Google Scholar

  • Matsuoka, Y., Mori, N., Kawahara, T. 2005. Genealogical use of chloroplast DNA variation for intraspecific studies of Aegilops tauschii Coss. Theor. Appl. Genet. 111 :265–271.

    Kawahara T. , 'Genealogical use of chloroplast DNA variation for intraspecific studies of Aegilops tauschii Coss ' (2005 ) 111 Theor. Appl. Genet. : 265 -271 .

    • Search Google Scholar

  • Molina-Cano, J.L., Russell, J.R., Moralejo, M.A., Escacena, J.L., Arias, G., Powell, W. 2005. Chloroplast DNA microsatellite analysis supports a polyphyletic origin for barley. Theor. Appl. Genet. 110 :613–619.

    Powell W. , 'Chloroplast DNA microsatellite analysis supports a polyphyletic origin for barley ' (2005 ) 110 Theor. Appl. Genet. : 613 -619 .

    • Search Google Scholar

  • Mogensen, H.L. 1996. The hows and whys of cytoplasmic inheritance in seed plants. Am. J. Bot. 83 :383–404.

    Mogensen H.L. , 'The hows and whys of cytoplasmic inheritance in seed plants ' (1996 ) 83 Am. J. Bot. : 383 -404 .

    • Search Google Scholar

  • Moxon, E.R., Wills, C. 1999. DNA microsatellites: agents of evolution? Sci. Am. 280 :94–99.

    Wills C. , 'DNA microsatellites: agents of evolution? ' (1999 ) 280 Sci. Am. : 94 -99 .

    • Search Google Scholar

  • Nishikawa, T., Vaughan, D.A., Kadowaki, K. 2005. Phylogenetic analysis of Oryza species, based on simple sequence repeats and their flanking nucleotide sequences from the mitochrondrial and chloroplast genomes. Theor. Appl. Genet. 110 :696–705.

    Kadowaki K. , 'Phylogenetic analysis of Oryza species, based on simple sequence repeats and their flanking nucleotide sequences from the mitochrondrial and chloroplast genomes ' (2005 ) 110 Theor. Appl. Genet. : 696 -705 .

    • Search Google Scholar

  • Ogihara, Y., Isono, K., Kojima, T., Endo, A., Hanaoka, M., Shiina, T., Terachi, T., Utsugi, S., Murata, M., Mori, N., Takumi, S., Ikeo, K., Gojobori, T., Murai, R., Murai, K., Matsuoka, Y., Ohnishi, Y., Tajiri, H., Tsunewaki, K. 2000. Chinese Spring wheat ( Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones. Plant Mol. Biol. Rep. 18 :243–253.

    Tsunewaki K. , 'Chinese Spring wheat (Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones ' (2000 ) 18 Plant Mol. Biol. Rep. : 243 -253 .

    • Search Google Scholar

  • Ogihara, Y., Ohsawa, T. 2002. Molecular analysis of the complete set of length mutation found in the plastomes of Triticum-Aegilops species. Genome 45 :956–962.

    Ohsawa T. , 'Molecular analysis of the complete set of length mutation found in the plastomes of Triticum-Aegilops species ' (2002 ) 45 Genome : 956 -962 .

    • Search Google Scholar

  • Powell, W., Morgante, M. 1995. Hypervariable microsatellites provide a general source of polymorphic DNA markers for the chloroplast genome. Curr. Biol. 5 :1023–1029.

    Morgante M. , 'Hypervariable microsatellites provide a general source of polymorphic DNA markers for the chloroplast genome ' (1995 ) 5 Curr. Biol. : 1023 -1029 .

    • Search Google Scholar

  • Provan, J., Powell, W., Hollingsworth, P.M. 2001. Chloroplast microsatellites: new tools for studies in plant ecology and evolution. Trends in Ecology & Evolution 16 :142–147.

    Hollingsworth P.M. , 'Chloroplast microsatellites: new tools for studies in plant ecology and evolution ' (2001 ) 16 Trends in Ecology & Evolution : 142 -147 .

    • Search Google Scholar

  • Rakoczy-Trojanowska, M., Bolibok, H. 2004. Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants. Cellular & Molecular Biology Letters. 9 :221–238.

    Bolibok H. , 'Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants ' (2004 ) 9 Cellular & Molecular Biology Letters : 221 -238 .

    • Search Google Scholar

  • Takeuchi, R., Kimura, S., Saetome, A., Sakaguchi, K. 2007. Biochemical properties of a plastidial DNA polymerase of rice. Plant Mol. Biol. 64 :601–611.

    Sakaguchi K. , 'Biochemical properties of a plastidial DNA polymerase of rice ' (2007 ) 64 Plant Mol. Biol. : 601 -611 .

    • Search Google Scholar

  • Tuvesson, I.K.D., Pedersen, S., Andersen, S.B. 1989. Nuclear genes affecting albinism in wheat ( Triticum aestivum L.) anther culture. Theor. Appl. Genet. 78 :879–883.

    Andersen S.B. , 'Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture ' (1989 ) 78 Theor. Appl. Genet. : 879 -883 .

    • Search Google Scholar

  • Varshney, R.K., Graner, A., Sorrells, M.E. 2005. Genic microsatellite markers in plants: Features and applications. TRENDS in Biotechnology 23 :48–55.

    Sorrells M.E. , 'Genic microsatellite markers in plants: Features and applications ' (2005 ) 23 TRENDS in Biotechnology : 48 -55 .

    • Search Google Scholar

  • Zhou, H., Konzak, C.F. 1992. Genetic control of green plant regeneration from anther culture of wheat. Genome 35 : 957–960.

    Konzak C.F. , 'Genetic control of green plant regeneration from anther culture of wheat ' (1992 ) 35 Genome : 957 -960 .

    • Search Google Scholar
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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
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1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
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Foundation		 1973
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per Year		 1
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per Year		 4
Founder Akadémiai Kiadó
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
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Springer Nature Switzerland AG
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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